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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (09): 1305-1312.doi: 10.3724/SP.J.1006.2015.01305

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

RNA Interference of OsGABA-T1 Gene Expression Induced GABA Accumulation in Rice Grain

ZHOU Lu, SHEN Bei-Bei, BAI Su-Yang, LIU Xi, JIANG Ling*, ZHAI Hu-Qu, WAN Jian-Min   

  1. Nanjing Agricultural University, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, China
  • Received:2015-02-02 Online:2015-09-12 Published:2015-09-12

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Abstract: γ-Aminobutyric acid (GABA) is a four-carbon non-protein amino acid and has been identified to have a function in reducing blood pressure. In order to increase GABA contents in rice grains, we constructed a RNA interference (RNAi) vector suppressing the expression of GABA transaminase 1 gene (OsGABA-T1). GABA transaminase is a key enzyme that converts GABA to succinate semialdehyde (SSA). The RNA interference vector was transformed into the callus of japonica cv. Ningjing 1. qRT-PCR analysis showed that OsGABA-T1 transcript abundance in the RNAi transgenic lines was effectively reduced, so did the homolog gene OsGABA-T2. The GABA contents of brown rice in T3 generation of RNAi lines were 13 times higher than these in Ningjing 1, so did in polished rice as well as brown rice and polished rice stored for four months of post-harvest in transgenic rice, while other main amino acid contents did not change much. In summary, this research provides a basis for breeding a rice variety which could contribute to reduce blood pressure due to the enrichment of GABA contents in rice seeds though effectively suppressing the expression of OsGABA-T1 gene by the RNA interference technique.

Key words: GABA, RNA interference, OsGABA-T1, Transgenic rice

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