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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (02): 170-179.doi: 10.3724/SP.J.1006.2016.00170

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Cloning of ZmPP6C Gene and Its Expression Patterns in Response to Light and Stress Treatments in Maize (Zea mays L.)

YUAN Huan-Huan1,2,**,SUN Guang-Hua1,2,**,YAN Lei 2,GUO Lin2,FAN Xiao-Cong1,2,XIAO Yang3,MENG Fan-Hua2,SONG Mei-Fang2,4,ZHAN Ke-Hui1,YANG Qing-hua1,*, YANG Jian-Ping1,2,*   

  1. 1 College of Agronomy, Henan Agricultural University/Collaborative Innovation Center of Henan Grain Crops, Zhengzhou 450002, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 4Beijing Radiation Center, Beijing 100875, China
  • Received:2015-08-27 Revised:2015-11-20 Online:2016-02-12 Published:2015-12-07
  • Supported by:

    This study was supported by the Natural Science Foundation (6151002), the Major Project of China on New Varieties of GMO Cultivation (2014ZX08010-002), the National Natural Science Foundation of China (31570268, 31170267) and the Agricultural Science and Technology Innovation Program (ASTIP) of CAAS.

Abstract:

PP6C is the catalytic subunits of Ser/Thr protein phosphatase 6 (PP6) gene, which plays important roles in auxin transport polarity, ABA (abscisic acid) signal transduction, flowering time control though light signaling pathway. To clarify structural characteristics of PP6C protein and the evolution relationships among plant PP6Chomologs, we cloned ZmPP6C gene by RT-PCR. The open reading frame (ORF) of ZmPP6C possesses 912 nucleotides and encodes 303 amino acid residues with one PP2Ac domain (the catalytic subunits of Ser/Thr protein phosphatase 2A). Phylogenetic analysis indicated that ZmPP6C belongs to the same branch with the PP6C of Sorghum bicolor, and shows high similarity to all PP6C proteins from other monocotyledons and dicotyledons. Further quantitative RT-PCR (qRT-PCR) assays indicated that ZmPP6C was highly expressed in leaf and lowly in stem, stamen, pulvinus, sheath, and pedical. ZmPP6C transcription abundances could respond to different light and circadian treatments (both long-day and short-day conditions), especially to the light transitions from the dark to far-red or red light condition. In addition, ZmPP6C transcription abundances were up-regulated by high osmosis, high salt and water logging. Our results suggested that ZmPP6C may be involved in light signaling pathway, flowering time control, and abiotic stress response in maize, and its roles in crop improvement are worthy of more exploration in the future.

Key words: Zea mays, ZmPP6C, Expression patterns, Phytochrome, Light signaling pathway, Abiotic stress

[1] Terol J, Bargues M, Carrasco P, Pérez-Alonso M, Paricio N. Molecular characterization and evolution of the protein phosphatase 2A B’ regulatory subunit family in plants. Plant Physiol, 2002, 129: 808–822



[2] Moorhead G B, Trinkle-Mulcahy L, Ulke-Lemée A. Emerging roles of nuclear protein phosphatases. Nat Rev Mol Cell Biol, 2007, 8: 234–244



[3] Cohen P T. Novel protein serine/threonine phosphatases: variety is the spice of life. Trends Biochem Sci, 1997, 22: 245–251



[4] Dai M, Xue Q, Mccray T, Margavage K, Chen F, Lee J H, Nezames C D, Guo L, Terzaghi W, Wan J, Deng X W, Wang H. The PP6 phosphatase regulates ABI5 phosphorylation and abscisic acid signaling in Arabidopsis. Plant Cell, 2013, 25: 517–534



[5] 刘钊, 贾霖, 贾盟, 关明俐, 曹英豪, 刘丽娟, 曹振伟, 李莉云, 刘国振. 水稻PP2Ac类磷酸酶蛋白质在盐胁迫下的表达. 中国农业科学, 2012, 45: 2339–2345



Liu Z, Jia L, Jia M, Guang L M, Cao Y H, Liu L J, Cao Z W, Li L Y, Liu G Z. Expression on profiling of rice PP2Ac type phosphatase proteins in seedlings under salt stressed conditions. Sci Agric Sin, 2012, 45: 2339–2345 (in Chinese with English abstract)



[6] Kim D H, Kang J G, Yang S S, Chung K S, Song P S, Park C M. A phytochrome-associated protein phosphatase 2A modulates light signals in flowering time control in Arabidopsis. Plant Cell, 2002, 14: 3043–3056



[7] Farkas I, Dombrádi V, Miskei M, Szabados L, Koncz C. Arabidopsis PPP family of serine /threonine phosphatases. Trends Plant Sci, 2007, 12: 169–176



[8] Gälweiler L, Guan C, Müller A, Wisman E, Mendgen K, Yephremov A, Palme K. Regulation of polar auxin transport by AtPIN1 in Arabidopsis vascular tissue. Science, 1998, 282: 2226–2230



[9] Chen R, Hilson P, Sedbrook J, Rosen E, Caspar T, Masson P H. The Arabidopsis thaliana AGRAVITROPIC 1 gene encodes a component of the polar-auxin-transport efflux carrier. Proc Natl Acad Sci USA, 1998, 95: 15112–15117



[10] Müller A, Guan C, Gälweiler L, Tänzler P, Huijser P, Marchant A, Parry G, Bennett M, Wisman E, Palme K. AtPIN2 defines a locus of Arabidopsis for root gravitropism control. EMBO J, 1998, 17: 6903–6911



[11] Friml J, Benková E, Blilou I, Wisniewska J, Hamann T, Ljung K, Woody S, Sandberg G, Scheres B, Jürgens G, Palme K. AtPIN4 mediates sink-driven auxin gradients and root patterning in Arabidopsis. Cell, 2002, 108: 661–673



[12] Petrásek J, Mravec J, Bouchard R, Blakeslee J J, Abas M, Seifertová D, Wisniewska J, Tadele Z, Kubes M, Covanová M, Dhonukshe P, Skupa P, Benková E, Perry L, Krecek P, Lee OR, Fink G R, Geisler M, Murphy A S, Luschnig C, Zazímalová E, Friml J. PIN proteins perform a rate-limiting function in cellular auxin efflux. Science, 2006, 312: 914–918



[13] Dai M, Zhang C, Kania U, Chen F, Xue Q, McCray T, Li G, Qin G, Wakeley M, Terzaghi W, Wan J, Zhao Y, Xu J, Friml J, Deng X W, Wang H. A PP6-type phosphatase holoenzyme directly regulates PIN phosphorylation and auxin efflux in Arabidopsis. Plant Cell, 2012, 24: 2497–2514



[14] Mauch-Mani B, Mauch F. The role of abscisic acid in plant-pathogen interactions. Curr Opin Plant Biol, 2005, 8: 409–414



[15] Fujita Y, Fujita M, Satoh R, Maruyama K, Parvez M M, Seki M, Hiratsu K, Ohme-Takagi M, Shinozaki K, Yamaguchi-Shinozaki K. AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis. Plant Cell, 2005, 17: 3470–3488



[16] Fujita Y, Fujita M, Shinozaki K, Yamaguchi-Shinozaki K. ABA-mediated transcriptional regulation in response to osmotic stress in plants. J Plant Res, 2011, 124: 509–525



[17] Hauser F, Waadt R, Schroeder J I. Evolution of abscisic acid synthesis and signaling mechanisms. Curr Biol, 2011, 21: R346–355



[18] Hattori T, Totsuka M, Hobo T, Kagaya Y, Yamamoto-Toyoda A. Experimentally determined sequence requirement of ACGT-containing abscisic acid response element. Plant Cell Physiol, 2002, 43: 136–140



[19] Busk P K, Pagès M. Regulation of abscisic acid induced transcription. Plant Mol Biol, 1998, 37: 425–435



[20] Finkelstein R R, Lynch T J. The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor. Plant Cell, 2000, 12: 599–609



[21] Lopez-Molina L, Chua N H. A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana. Plant Cell Physiol, 2000, 41: 541–547



[22] Liu X B, Zhang X Y, Wang Y X, Sui Y Y, Zhang S L, Herbert S J, Ding G. Soil degradation: a problem threatening the sustainable development of agriculture in Northeast China. Plant Soil Environ, 2010, 56: 87–97



[23] Gao Y, Jiang W, Dai Y, Xiao N, Zhang C, Li H, Lu Y, Wu M, Tao X, Deng D, Chen J. A maize phytochrome interacting factor 3 improves drought and salt stress tolerance in rice. Plant Mol Biol, 2015, 87: 413–428



[24] Xu Z S, Ni Z Y, Li Z Y, Li L C, Chen M, Gao D Y, Yu X D, Liu P, Ma Y Z. Isolation and functional characterization of HvDREB1—a gene encoding a dehydration-responsive element binding protein in Hordeum vulgare. J Plant Res, 2009, 122: 121–130



[25] Tian S J, Mao X G, Zhang H Y, Chen S S, Zhai C C, Yang S M, Jing R L. Cloning and characterization of TaSnRK2.3, a novel SnRK2 gene in common wheat. J Exp Bot, 2013, 64: 2063–2080



[26] Liu Z J, Yang X G, Hubbard K G, Lin X M. Maize potential yields and yield gaps in the changing climate of northeast China. Global Change Biol, 2012, 18: 3441–3454



[27] Yang X, Lin E D, Ma S M, Ju H, Guo L P, Xiong W, Li Y, Xu Y L. Adaptation of agriculture to warming in Northeast China. Clim Change, 2007, 84: 45–58



[28] Sun H, Tonks N K. The coordinated action of protein tyrosine phosphatases and kinases in cell signaling. Trends Biochem Sci, 1994, 19: 480–485



[29] Hanada M, Ninomiya-Tsuji J, Komaki K, Ohnishi M, Katsura K, Kanamaru R, Matsumoto K, Tamura S. Regulation of the TAK1 signaling pathway by protein phosphatase 2C. J Biol Chem, 2001, 276: 5753–5759



[30] 翁华, 冉亮, 魏群. 植物蛋白磷酸酶及其在植物抗逆中的作用. 植物学通报, 2003, 20: 609–615



Weng H, Ran L, Wei Q. Protein phosphatases and their functions in plant response to environmental stress. Chin Bull Bot, 2003, 20: 609–615 (in Chinese with English abstract)



[31] Rajeevan M S, Ranamukhaarachi D G, Vernon S D, Unger E R. Use of real-time quantitative PCR to validate the results of cDNA array and differential display PCR technologies. Methods, 2001, 25: 443–451



[32] Shi Y. Serine/Threonine phosphatases: mechanism through structure. Cell, 2009, 139: 468–484



[33] Fankhauser C, Chory J. Light control of plant development. Annu Rev Cell Dev Biol, 1997, 13: 203–229



[34] Neff M M, Fankhauser C, Chory J. Light: An indicator of time and place. Genes Dev, 2000, 14: 257–271



[35] Ma L, Li J, Qu L, Hager J, Chen Z, Zhao H, Deng X W. Light control of Arabidopsis development entails coordinated regulation of genome expression and cellular pathways. Plant Cell, 2001, 13, 2589–2607



[36] 李潮海, 刘奎. 不同产量水平玉米杂交种生育后期光合效率比较分析. 作物学报, 2002, 28: 379–383



Li C H, Liu K. Analysis of photosynthesis efficiency of maize hybrids with different yield in the later growth stage. Acta Agron Sin, 2002, 28: 379–383 (in Chinese with English abstract)



[37] Smith H. Phytochrome transgenics: functional, ecological and biotechnological applications. Semin Cell Biol. 1994, 5: 315–325



[38] Bae G, Choi G. Decoding of light signals by plant phytochromes and their interacting proteins. Annu Rev Plant Biol, 2008, 59: 281–311



[39] Li J, Li G, Wang H, Deng X W. Phytochrome signaling mechanisms. America: American Society of Plant Biologists,2011. pp1-26



[40] Quail P H. Phytochrome photosensory signalling networks. Nat Rev Mol Cell Biol, 2002, 3: 85–93



[41] 詹克慧, 李志勇, 侯佩, 习雨琳, 肖阳, 孟凡华, 杨建平. 利用修饰光敏色素信号途径进行品种改良的可行性. 中国农业科学, 2012, 45: 3249−3255



Zhan K H, Li Z Y, Hou P, Xi Y L, Xiao Y, Meng F H, Yang J P. A new strategy for crop improvement through modification of phytochrome signaling pathways. Sci Agric Sin, 2012, 45: 3249−3255



[42] Boylan M T, Quil P H. Oat phytochrome is biologically active in transgenic tomatoes. Plant Cell, 1989, 1: 765−773



[43] Nagatani A, Kay S A, Deak M, Chua N H, Furuya M. Rice type I phytochrome regulates hypocotyl elongation in transgenic tobacco seedlings. Proc Natl Acad Sci USA, 1991, 88: 5207−5211



[44] Garg A K, Sawers R J, Wang H, Kim J K, Walker J M, Brutnell T P, Parthasarathy M V, Vierstra R D, Wu R J. Light-regulated overexpression of an Arabidopsis phytochrome A gene in rice alters plant architecture and increases grain yield. Planta, 2006, 223: 627−636



[45] Thiele A, Herold M, Lenk I, Quail P H, Gatz C. Heterologous expression of Arabidopsis phytochrome B in transgenic potato influences photosynthetic performance and tuber development. Plant Physiology, 1999, 120: 73−815



[46] Putterill J, Robson F, Lee K, Simon R, Coupland G. The CONSTANS gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors. Cell, 1995, 80: 847–857



[47] Suárez-López P, Wheatley K, Robson F, Onouchi H, Valverde F, Coupland G. CONSTANS mediates between the circadian clock and the control of flowering in Arabidopsis. Nature, 2001, 410: 1116–1120



[48] Guo H, Yang H, Mockder T C, Lin C. Regulation of flowering time by Arabidopsis photoreceptors. Science, 1998, 279: 1360–1363



[49] Pineiro R P, Coupland G. The control of flowering time and floral identity in Arabidopsis. Plant Physiol, 1998, 17: 1–8



[50] Samach A, Onouchi H, Gold S E, Ditta G S, Schwarz-Sommer Z, Yanofsky M F, Coupland G. Distinct roles of CONSTANS target genes in reproductive development in Arabidopsis. Science, 2000, 288: 1613–1616



[51] 王翠玲, 程芳芳, 孙朝晖, 库丽霞, 陈晓, 陈彦惠. 玉米光周期敏感性的遗转特性及相关基因的研究进展. 玉米科学, 2008, 16: 11–14



Wang C L, Cheng F F, Sun Z H, Ku L X, Chen X, Chen Y H. Advances in genetic research and related genes of photoperiod sensitivity in maize. J Maize Sci, 2008, 16: 11–14 (in Chinese with English abstract)



[52] 李思远, 陈晓, 王新涛, 陈彦惠. 玉米光周期敏感类Hd6基因的克隆和实时定量表达分析. 作物学报, 2008, 34: 713−717



Li S Y, Chen X, Wang X T, Chen Y H. Clone and quantitative analysis by real-time RT-PCR of photoperiod sensitive gene Hd6-like in maize. Acta Agron Sin, 2008, 34: 713−717 (in Chinese with English abstract)



[53] Yu R M, Zhou Y, Xu Z F, Chye M L, Kong R Y. Two genes encoding protein phosphatase 2A catalytic subunits are differentially expressed in rice. Plant Mol Biol, 2003, 51: 295–311

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