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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (05): 714-724.doi: 10.3724/SP.J.1006.2016.00714

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Soluble Oligosaccharide and Small Heat Shock Protein Correlated with Seed Germination and Vigor during Hybrid Rice Seed Maturation

ZHU Li-Wei1,2,**,CAO Dong-Dong3,**,FU Yu-Ying1,HU Qi-Juan1,LI Zhan1,GUAN Ya-Jing1,HU Wei-Min1,HU Jin1,*   

  1. 1Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; 2Research Center of Buckwheat Industry Technology, Guizhou Normal University, Guiyang 550001, China; 3Zhejiang Agriculture Science Limited Company, Hangzhou310058, China
  • Received:2015-08-14 Revised:2016-03-02 Online:2016-05-12 Published:2016-03-11
  • Contact: Hu Jin, E-mail: jhu@zju.edu.cn**同等贡献(Contributed equally to this work) E-mail:liweib0401001@163.com
  • Supported by:

    This study was supported by the China Special Fund for Agro-Scientific Research in the Public Interest (201203052), the Subject Fund of ZhejiangProvince Science and Technology Hall (2013C02005, 2013C32023), the Natural Sciences Fund of ZhejiangProvince (LZ14C130002, LY15C130002), and JiangsuCollaborative Innovation Center for Modern Crop Production.

Abstract:

To study the relationship between seed soluble oligosaccharide, small heat shock protein (sHSPs) and seed vigor, we systematically studied their changes during hybrid rice seed maturity.The mean fuzzy subordinate function values of germination percentage, germination energy, germination index, vigor index and mean germination time, root number, root length, shoot length and seedling weight were analyzed. The resultsindicated thatseeds harvesting at 19 to 34 days after pollination (DAP) had a higher viability and vigor. The seed moisture content had a decline trend, with a decrease from 48.2% at 10 DAP to 23.9% at 22 DAP. The raffinose content increased significantly, the fructose content kept decreasing, the contents of stachyose and glucose significantly increased firstly and then decreased during seed development. The relative expression levels of 64SHsp18.0 and Os03g0267200 genes increased significantly from 10 to 19 DAP, and then decreased. The moisture content,the contents of raffinose,stachyose andsucrose, thousand seed weight of fresh seeds and dry seeds,relative transcriptions of 64SHsp18.0 and Os03g0267200 genes were all significantly correlated with seed germination during seed maturity.

Key words: Hybrid rice, Seed, Maturity, sHSPs, Oligosaccharide, Vigor

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