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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (06): 820-831.doi: 10.3724/SP.J.1006.2016.00820

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping QTLs For Wheat Panicle Traits with High Density SNP Genetic Map

LIU Kai,DENG Zhi-Ying,LI Qing-Fang,ZHANG Ying,SUN Cai-Ling,TIAN Ji-Chun*,CHEN Jian-Sheng*   

  1. Group of Wheat Quality Breeding, College of Agronomy, Shandong Agricultural University / State Key Laboratory of Crop Biology, Tai’an 271018,China
  • Received:2015-11-09 Revised:2016-03-14 Online:2016-06-12 Published:2016-03-21
  • Contact: 陈建省, E-mail: jshch@sdau.edu.cn, Tel: 0538-8249236; 田纪春, E-mail: jctian@sdau.edu.cn E-mail:liukaiyouxiang@163.com
  • Supported by:

    This study was supported by the Natural Science Foundation of Shandong Province, China (2015ZRB01179 and ZR2013CM004) and the Project of Germplasm Resource Enhancement in Shandong Province.

Abstract:

Panicle traits of wheat are closely correlated between each other,of them grain number per spike and 1000-grain weight are important components of grain yield. In this study,we mapped quantitative trait loci (QTLs) associated with wheatspike traits using a recombinant inbred line (RIL) population (173 lines of F8:9) derived from a cross of Shannong 01-35×Gaocheng 9411.The phenotypic data were collected in five environments and the high density genetic map was constructedusing90k SNP array,DArT technology and traditional molecular markers. In a combination analysis of five environments, many additive QTLs were detected includingseven for 1000-grain weight,eight for spike length,threefor grain number per spike, five for fertile spikelet number per spike, three for sterile spikelet number per spike,four for spikelet number per spike, and six for spike density.Some QTLs showed high rates of phenotypic variation explained (PVE). For example, the PVE of QTLs for 1000-grain weight on 1B, 4B, 5B and 6A ranged from 6.00% to 36.30%,with the favorable alleles from the large-grain parent Shannong 01-35; the PVE of QTLs for spike length ranged from 14.34% to 25.44%, and thatfor sterile spikelet number per spike from 8.70% to 37.70%. In addition to additive loci,32 pairs of epistatic QTLs were detected, which explained 0.05–1.05% of the phenotypic variations. The marker interval between EX_C101685 and RAC875_C27536 on chromosome 4B showed pleiotropic effectsin 1000-grain weight, spike length, grain number per spike, fertile spike number, sterile spikelet number, and spikelet number per spike, with the PVE ranging from 5.40% to 37.70%. There stable main QTLs were detected in multiple environments. Besides, markerinterval between wPt-0959 and TaGw2-CAPS on 6Ahad a locus controllingboth 1000-grain weight and spikelet number per spike. These results are valuable in developing molecular markers, fine mapping and cloning genes for spike traits in wheat.

Key words: Common wheat, 90k array, QTL mapping, Panicle, SNP

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