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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (06): 813-819.doi: 10.3724/SP.J.1006.2016.00813

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Phenotypic Analysis and Gene Mapping of degenerated hull 3 (dh3) Mutant in Rice (Oryza sativa L.)

LONG Jue-Chen,ZHUANG Hui,CHEN Huan,WANG Ling,SHEN Ya-Lin,ZENG Xiao-Qin,CUI Xin-Yun,SANG Xian-Chun,HE Guang-Hua,LI Yun-Feng*   

  1. Rice Research Institute, Southwest University / Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Chongqing 400716, China?
  • Received:2015-12-09 Revised:2016-03-14 Online:2016-06-12 Published:2016-03-21
  • Contact: 李云峰, E-mail: liyf1980@swu.edu.cn E-mail:598161800@qq.com
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (31271304), Natural Science Foundation project of CQ (CSTC2012JJB80005), and the Fundamental Research Funds for the Central Universities (XDJK2012A001).

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Abstract:

The floral organ development directly influences the yield and quality of rice. In this study, we reported a mutant degenerated hull 3 (dh3), derived from ethylmethane sulfonate (EMS)-treated Jinhui10 (Oryza sativa L. ssp. indica). The manifestation of dh3 mutant was degradation and narrowing in lemma and palea, losting the ability of close-up. In some seriously mutated florets, the lemma even degenerated in an awn-like shape, the margin region of palea and lodicules became narrow, even fused. Genetic analysis indicated that the dh3 character is controlled by a recessive gene. By using 356 F2 mutants derived from a cross between sterile line Xinong 1A and dh3, DH3 was mapped between SSR markers RM27706 and RM27709 on chromosome 12 in rice, with a physical distance of 44.72 kb. There is no report of known functional gene in this zone. The results will shed light on the future clone and function analysis of DH3 gene.

Key words: Rice (Oryza sativa), degenerated hull, Phenotypic analysis, Gene mapping

[1] Bowman J L, Smyth D R, Meyerowitz E M. Genetic interactions among floral homeotic genes of Arabidopsis. Development, 1991, 112: 1–20
[2] Coen E S, Meyerowitz E M. The war of the whorls: genetic interactions controlling flower development. Nature, 1991, 353: 31–37
[3] Angenent G C, Franken J, Busscher M, Dijken A, Went J L, Dons H J, Tunen A J. A novel class of MADS box genes is involved in ovule development in Petunia. Plant Cell, 1995, 7: 1569–1582
[4] Pelaz S, Ditta G S, Baumann E, Wisman E, Yanofsky M F. B and C floral organ identity functions require SEPALLATA MADS-box genes. Nature, 2000, 405: 200–203
[5] Wang K J, Tang D, Hong L, Xu W Y, Huang J, Li M, Gu M H, Xue Y B, Cheng Z K. DEP and AFO regulate reproductive habit in rice. PloS Genet, 2010, 6: e1000818
[6] Jeon J S, Jang S, Lee S, Nam J, Kim C, Lee S H, Chung Y Y, Kim S R, Lee Y H, Cho Y G, An G. leafy hull sterile 1 is a homeotic mutation in a rice mads box gene affecting rice flower development. Plant Cell, 2000, 12: 871–884
[7] Gao X C, Liang W Q, Yin C S, Ji S M, Wang H M, Su X, Guo C C, Kong H Z, Xue H W, Zhang D B. The SEPALLATA-like gene OsMADS34 is required for rice inflorescence and spikelet development. Plant Physiol, 2010, 153: 728–740
[8] Duan Y L, Diao Z J, Liu H Q, Cai M S, Wang F, Lan T, Wu W R. Molecular cloning and functional characterization of OsJAG gene based on a complete-deletion mutant in rice (Oryza sativa L.). Plant Mol Biol, 2010, 74: 605–615
 [9] Li A, Zhang Y, Wu X, Tang W, Wu R, Dai Z, Liu G, Zhang H, Wu C. DH1, a LOB domain-like protein required for glume formation in rice. Plant Mol Biol, 2008, 66: 491–502
[10] Li X J, Sun L J, Tan L B, Liu F X, Zhu Z F, Fu Y C, Sun X Y, Sun X W, Xie D X. TH1, a DUF640 domain-like gene controls lemma and palea development in rice. Plant Mol Biol, 2012, 78: 351–359
[11] Shinnosuk O, Mayumi K, Maiko S, Akio M, Hirohiko H, Uchida E, Yasuo N, Hitoshi Y. MOSAIC FLORAL ORGANS1, an AGL6-Like MADS box gene, regulates floral organ identity and meristem fate in rice. Plant Cell, 2009, 21: 3008–3025
[12]Sang X C, Li Y F, Luo Z K, Ren D Y, Fang L K, Wang N, Zhao F M, Ling Y H, Yang Z L, Liu Y S, He G H. CHIMERIC FLORAL ORGANS1, encoding a monocot-specific mads box protein, regulates floral organ identity in rice. Plant Physiol, 2012, 160: 788–807
[13] Jin Y, Luo Q, Tong H N, Wang A J, Cheng Z J, Tang J F, Li D Y, Zhao X F, Li X B, Wan J M, Jiao Y L, Chu C C, Zhu L H. An AT-hook gene is required for palea formation and floral organ number control in rice. Dev Biol, 2011, 359: 277–288
[14] Zheng M, Wang Y, Wang Y, Wang C, Ren Y, Lü J, Peng C, Wu T, Liu K, Zhao S, Liu X, Guo X, Jiang L, Terzaghi W, Wan J. DEFORMED FLORAL ORGAN1 (DFO1) regulates floral organ identity by epigenetically repressing the expression of OsMADS58 in rice (Oryza sativa). New Phytol, 2015, 206: 1476–1490
[15] Yan D, Zhang X, Zhang L, Ye S, Zeng L, Liu J, Li Q, He Z. CURVED CHIMERIC PALEA 1 encoding an EMF1-like protein maintains epigenetic repression of OsMADS58 in rice palea development. Plant J, 2015, 82: 12–24
[16] Toriba T, Takuya S, Takahiro Y, Yoshihiro O, Hirokazu T, Hirano H. Distinct regulation of adaxial-abaxial polarity in anther patterning in rice. Plant Cell, 2010, 22: 1452–1462
[17] Song X W, Wang D K, Ma L J, Chen Z Y, Li P C, Cui X, Liu C Y, Cao S Y. Rice RNA-dependent RNA polymerase 6 acts in small RNA biogenesis and spikelet development. Plant J, 2012, 71: 378–389
[18] Liu B, Chen Z, Song X, Liu C, Cui X, Zhao X F, Fang J, Xu W, Zhang H, Wang X, Chu C, Deng X, Xue Y B, Cao X F. Oryza sativa Dicer-like 4 reveals a key role for small interfering rna silencing in plant development. Plant Cell, 2007, 19: 2705–2718
[19] Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregation analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832
[20] Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321–4325
[21] Luo Z K, Yang Z L, Zhong B Q, Li Y F, Xie R, Zhao F M, Ling Y H, He G H. Genetic analysis and fine mapping of a dynamic rolled leaf gene RL10(t) in rice (Oryza sativa L.). Genome, 2007, 50: 811–817
[22] Itoh J, Nonomura K, Ikeda K, Yamaki S, Inukai Y, Yamagishi H, Kitano H, Nagato Y. Rice plant development: from zygote to spikelet. Plant Cell Physiol, 2005, 46: 23–47
[23] Timmermans M C, Schultes N P, Jankovsky J P, Nelson T. LEAFBLADELESS1 is required for dorsoventrality of lateral organs in maize. Development, 1998, 125: 2813–2823
[24] Nogueira F T, Madi S, Chitwood D H, Juarez M T, Timmermans M C. Two small regulatory RNAs establish opposing fates of a developmental axis. Genes & Development, 2007, 21: 750–755
[25] Chitwood D H, Guo M, Nogueira F T, Timmermans M C. Establishing leaf polarity: the role of small RNAs and positional signals in the shoot apex. Development, 2007, 134: 813–823
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