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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (06): 813-819.doi: 10.3724/SP.J.1006.2016.00813

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Phenotypic Analysis and Gene Mapping of degenerated hull 3 (dh3) Mutant in Rice (Oryza sativa L.)

LONG Jue-Chen,ZHUANG Hui,CHEN Huan,WANG Ling,SHEN Ya-Lin,ZENG Xiao-Qin,CUI Xin-Yun,SANG Xian-Chun,HE Guang-Hua,LI Yun-Feng*   

  1. Rice Research Institute, Southwest University / Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Chongqing 400716, China?
  • Received:2015-12-09 Revised:2016-03-14 Online:2016-06-12 Published:2016-03-21
  • Contact: 李云峰, E-mail: liyf1980@swu.edu.cn E-mail:598161800@qq.com
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (31271304), Natural Science Foundation project of CQ (CSTC2012JJB80005), and the Fundamental Research Funds for the Central Universities (XDJK2012A001).

Abstract:

The floral organ development directly influences the yield and quality of rice. In this study, we reported a mutant degenerated hull 3 (dh3), derived from ethylmethane sulfonate (EMS)-treated Jinhui10 (Oryza sativa L. ssp. indica). The manifestation of dh3 mutant was degradation and narrowing in lemma and palea, losting the ability of close-up. In some seriously mutated florets, the lemma even degenerated in an awn-like shape, the margin region of palea and lodicules became narrow, even fused. Genetic analysis indicated that the dh3 character is controlled by a recessive gene. By using 356 F2 mutants derived from a cross between sterile line Xinong 1A and dh3, DH3 was mapped between SSR markers RM27706 and RM27709 on chromosome 12 in rice, with a physical distance of 44.72 kb. There is no report of known functional gene in this zone. The results will shed light on the future clone and function analysis of DH3 gene.

Key words: Rice (Oryza sativa), degenerated hull, Phenotypic analysis, Gene mapping

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