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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (11): 1609-1619.doi: 10.3724/SP.J.1006.2016.01609

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Construction and Genetic Evaluation of Chromosome Segment Substitution Linesin Tobacco(NicotianatabacumL.)

TONG Zhi-Jun, JIAO Fang-Chan, FANG Dun-Huang, CHEN Xue-Jun, WU Xing-Fu, ZENG Jian-Min, XIE He, ZHANG Yi-Han, and XIAO Bing-Guang*   

  1. Yunnan Academy of Tobacco Agricultural Sciences / Key Laboratory of Tobacco Biotechnological Breeding / National Tobacco Genetic Engineering Research Center, Kunming 650021, China
  • Received:2016-03-09 Revised:2016-07-11 Online:2016-11-12 Published:2016-07-28
  • Contact: 肖炳光,E-mail:xiaobg@263.net E-mail:tzj861@163.com
  • Supported by:

    ThisstudywassupportedbyChinaNationalTobaccoCompany(2014TBB01and110201301006(JY-06))andYunnanTobaccoCompany(2014YN18and2013YN01).

Abstract:

A set of chromosome segment substitution lines (CSSLs) of tobacco (Nicotiana tabacum L.) were first developed by molecular marker assisted selection (MAS) and successive backcrossing with Y3, the flue-cured tobacco germplasm with comprehensive traits as the recipient parent, and two common tobacco cultivars Beinhart1000-1 and K326 as the donor parents. The cigar tobacco cultivar Beinhart1000-1 carries a variety of resistance traits including black shank (race 0 and race 1) and brown spot resistance, while the flue-cured tobacco K326 is a commercial cultivar with high quality. In 256 CSSLs, a total of 377 substituted segments derived from donor Beinhart1000-1 and K326 with genetic background of Y3 distributed on 24 linkage groups. Each CSSL contained only 1–5 substituted segments and the length of substituted segments ranged from 0.05 to 36.88 cM with an average of 7.75 cM. The total length of the overlapped substituted segments was 2922.57 cM, which was 2.61 times of the whole tobacco genome length. And the CSSLs covered length was 1114.32 cM, with a covered ratio of 99.45% in the recurrent tobacco genome. The CSSLs constructed in this study are excellent genetic materials for gene mapping, QTL analysis of complicated traits, and developing varieties by marker assisted selection in Nicotiana tabacum L.

Key words: Tobacco(Nicotianatabacum L.), Chromosomesegmentsubstitutionlines(CSSLs), SSR, Molecularmarkerassistedselection(MAS)

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