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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (02): 218-225.doi: 10.3724/SP.J.1006.2017.00218

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Screening of AhHDA1 Interacting-Protein AhGLK and Characterization in Peanut (Arachis hypogaea L.)

LI Mei-Juan,SU Liang-Chen,LIU Shuai,LI Xiao-Yun*,LI Ling*   

  1. Guangdong Key Laboratory of Biotechnology for Plant Development, South China Normal University, Guangzhou, 510631, China
  • Received:2016-04-02 Revised:2016-09-18 Online:2017-02-12 Published:2016-09-28
  • Contact: 李晓云, E-mail: xiaoyun5893@qq.com, Tel: 020-85211378; 李玲, E-mail: liling502@126.com, Tel: 020-85211378 E-mail:929722415@qq.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31471422, 31671600).

Abstract:

With AhHDA1 as bait, the peanut (Arachis hypogaea L.) cDNA library was screened and characteristics of the interacting-protein was analysed, getting a protein of Golden 2-like (AhGLK) interacted with AhHDA1 which was proved by yeast two-hybrid experiments, and further confirmed by bimolecular fluorescence complementation (BiFC) assay. Bioinformatics software analysis indicated that AhGLK contained a MYB conserved domain having high homology with GsGLK (Glyine soja) and AtGLK (Arabidopsis thaliana). AhGLK was found to be located in nucleus and testified as a transcriptional activation factor. Tissue specific analysis showed that AhGLK accumulated in leaves mainly, and the expression was down-regulated by 30% PEG treatment. The results lay a foundation for further study on AhGLK, which might be involved in drought stress resistance of peanut.

Key words: Peanut, AhHDA1, Interacting protein, AhGLK, Transcriptional activity

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