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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (11): 1658-1666.

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of Waterlogging on Bt Protein Content and Nitrogen Metabolism in Square of Bt Cotton

LI Yuan1, LI Ya-Bing2, HU Da-Peng1, WANG Jun1, HENG Li1, ZHANG Xiang1, CHEN Yuan1, CHEN De-Hua1,*   

  1. 1 Jiangsu Provincial Key Laboratory of Crops Genetics and Physiology / Yangzhou University, Yangzhou 225009, China; 2 State Key Laboratory of Cotton Biology / Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China
  • Received:2017-02-22 Revised:2017-07-23 Online:2017-11-12 Published:2017-08-09
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31471435, 31301263, 31671613), Jiangsu “Threeinnovation”Agricultural Project [SXGC(2014)317, (2016)320], the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and the Project of Planting Cotton Technology Standardization Research and Demonstration.

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Abstract:

Using Bt cotton conventional cultivar Sikang 1 and hybrid Sikang 3 as test materials, the experiments of water stress at different levels (relative soil water capacity at 90%, 100%, and 2 cm waterlogging above the soil surface respectively) and 90% soil water capacity remained 24–120 hours were conducted to investigate the square Bt insecticidal protein expression and nitrogen metabolism in bud developmental stage. The soil waterlogging treatments with three levels reduced the square Bt insecticidal protein content by 36.5%, 42.1%, and 51.4% for conventional cultivar Sikang 1 and by 19.2%, 57.2%, and 64.5% for hybrid Sikang 3, respectively. The continuous stress of 96 h in the 90% soil water capacity treatment decreased the square Bt insecticidal protein content significantly, which was 20.8% for Sikang 1 and 17.6% for Sikang 3, the longer the stress sustained, the more the Bt toxin content declined. The soluble protein content, NR, GOT, GPT, GS, and GOGAT activities decreased, and the free amino acids content, peptidase and protease activities increased under different levels of soil waterlogging and continuous stress of 90% soil water capacity stress. Therefore, the reduced protein synthesis ability, and enhanced protein degradation ability may decrease the square soluble protein content including Bt insecticidal protein content, resulting in reduction of the square insect resistance.

Key words: Bt cotton, Water logging, Insecticidal protein, Nitrogen metabolism

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