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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (01): 99-106.doi: 10.3724/SP.J.1006.2012.00099


Response of Some Key Enzyme Activities Involved in Nitrogen Metabolism to High Temperature at Filling Stage and Its Relation to Storage Protein Accumulation in Rice Grain

CAO Zhen-Zhen,ZHANG Qi-Fang,WEI Ke-Su,YANG Wei-Li,LIU Gaung-Kuai,CHENG Fang-Min*   

  1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China
  • Received:2011-05-20 Revised:2011-10-12 Online:2012-01-12 Published:2011-11-07
  • Contact: 程方民, E-mail: chengfm@zju.edu.cn

Abstract: The influence of high temperature after flowering period on the activities of some enzymes, including glutamine synthetase (GS), glutamic-oxaloacetic transaminase (GOT)and glutamic-pyruvic transaminase (GPT) in developing grains, and also its relation to grain storage protein and amino acid accumulations were investigated by using two early-indica rice cultivars grown under two temperature regimes of daily average temperature 32℃ and 22℃, respectively, and expression responses of two GS isoform genes to temperature were detected by real-time fluorescence quantitative PCR(FQ-PCR). The results indicated that the effects of high temperature after flowering period on GOT and GPT activities showed the similar trend, with increasing at early grain filling stage and decreasing at late grain filling stage. However, the increase f total contents of crude protein and various amino acids under high temperature after flowering period were not always due to the enhancement of grain nitrogen transport capacity and protein synthesis ability. GS activity under high temperature was generally higher than that under temperature in the same period. GS2,one of the isoforms of GS gene, was highly expressed in rice endosperm. The expression levels of GS2 was even higher than that of GS1 in late grain filling stage, while high temperature stress regulated GS activity through changing GS1 and GS2 genes transcription level in grain.

Key words: Rice (Oryza sativa L.), High temperature, Nitrogen metabolism, Enzyme activity, Protein accumulation

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