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Acta Agronomica Sinica ›› 2018, Vol. 44 ›› Issue (9): 1393-1399.doi: 10.3724/SP.J.1006.2018.01393

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Effect of Exogenous Nitric Oxide Donor on Carbon Assimilation and Antioxidant System in Leaves of Maize Seedlings under PEG-induced Water Deficit Stress

Qing-Hua YANG(),Bo-Yuan ZHENG,Lei-Lei LI,Shuang-Jie JIA,Xin-Pei HAN,Jia-Meng GUO,Yong-Chao WANG,Rui-Xin SHAO()   

  1. College of Agronomy, Henan Agricultural University, Zhengzhou 450046, Henan, China
  • Received:2017-12-22 Accepted:2018-06-12 Online:2018-09-10 Published:2018-07-02
  • Contact: Rui-Xin SHAO E-mail:yangqh2010@163.com;shao_rui_xin@126.com
  • Supported by:
    This study was supported by the National Natural Science Foundation of China (31401304)

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Abstract:

The objective of this study was to explore the effect of exogenous nitric oxide (NO) donor (sodium nitroprusside, SNP) on key enzymes of carbon assimilation and antioxidant system of maize leaves under water deficit and its regulation mechanism. In this experiment, 20% PEG-6000 was used to stimulate water deficit stress, exogenous SNP was added into root rhizosphere of seedlings in maize variety Zhuyu 309. After three days of stresses, the changes of Rubisco and RCA activities and their gene level, antioxidase activity and their isoenzyme spectrum level were investigated. The expression levels of rbc L, rbc S, rca β were increased significantly, especially for rbc S that was increased the most by 1.86 fold, which resulted in up-regulation of Rubisco and RCA activities by 32.7% and 14.67% under exogenous SNP plus PEG stress. In addition, SNP enhanced the activity of SOD, POD, CAT, and the width in their isoenzyme spectrum, resulting in significant reduction of ROS accumulation. These results suggested that NO could increase photosynthetic carbon assimilation capacity and antioxidase activity, alleviate the damage of ROS burst on the cell membrane, which enhances PEG-simulated water deficit resistance of maize seedlings.

Key words: exogenous nitric oxide, water deficit, maize seedlings, carbon assimilation, antioxidant system

Table 1

Primers used in this study"

引物名称
Primer name		 序列
Sequence (5'-3')		 Tm
(°C)
actin-F CTGAACCCCAAGGCAAACA 59.0
actin-R ACTGGCGTACAGGGAAAGAA 57.3
rca β-F TCCTTGAGACCTTCTTGACGG 59.8
rca β-R ATCGCCTTGAACCTGCTGT 57.8
rbc L-F CCGTTTCGTCTTTTGTGCC 58.9
rbc L-R TGCGGTGAATCCTCCTGTT 58.3
rbc S-F CGCTACTGGACCATGTGGAA 59.1
rbc S-R ACTGCGTCTGCTTGATGTTGT 58.1

Fig. 1

Effects of exogenous NO on the Rubisco activity (A) and RCA activity (B) in leaves of maize seedlings under drought stressTreatments including CK, SNP, PEG, and SNP + PEG indicate 0% PEG, 100 μmol L-1 SNP, 20% PEG, and 100 μmol L-1 SNP + 20% PEG, respectively."

Fig. 2

Effects of exogenous NO on the expression of rbc S (A), rbc L (B), and rca β (C) in leaves of maize seedlings under drought stressTreatments including CK, SNP, PEG, and SNP + PEG indicate 0% PEG, 100 μmol L-1 SNP, 20% PEG, and 100 μmol L-1 SNP + 20% PEG, respectively."

Fig. 3

Effects of exogenous NO on O2-(A) and H2O2(B) contents in leaves of maize seedlings under drought stressTreatments including CK, SNP, PEG, and SNP + PEG indicate 0% PEG, 100 μmol L-1 SNP, 20% PEG, and 100 μmol L-1 SNP + 20% PEG, respectively."

Fig. 4

Effects of exogenous NO on SOD activity (A) and SOD isoenzyme (B) in leaves of maize seedlings under drought stressTreatments including CK, SNP, PEG, and SNP + PEG indicate 0% PEG, 100 μmol L-1 SNP, 20% PEG, and 100 μmol L-1 SNP + 20% PEG, respectively."

Fig. 5

Effects of exogenous NO on POD activity (A) and POD isoenzyme (B) in leaves of maize seedlings under drought stressTreatments including CK, SNP, PEG, and SNP + PEG indicate 0% PEG, 100 μmol L-1 SNP, 20% PEG, and 100 μmol L-1 SNP + 20% PEG, respectively."

Fig. 6

Effects of exogenous NO on CAT activity (A) and CAT isoenzyme (B) in leaves of maize seedlings under drought stressTreatments including CK, SNP, PEG, and SNP + PEG indicate 0% PEG, 100 μmol L-1 SNP, 20% PEG, and 100 μmol L-1 SNP + 20% PEG, respectively."

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