增效缩节安化学封顶对棉花主茎生长的影响及其相关机制

doi:10.3724/SP.J.1006.2018.01837

Abstract

Abstract:

The plant growth regulator mepiquat chloride (1,1-dimethyl piperidinium chloride, DPC) has been successfully and worldwide used in cotton production. Fortified mepiquat chloride is a type of aqueous formulation containing 25% DPC (referred to DPC ⁺ hereafter), which can slightly damage young tissues of epidermis. DPC ⁺ has shown potential in cotton chemical topping in China, and may replace the conventional manual topping in future. In order to investigate the mechanism of cotton chemical topping with DPC ⁺, this field study was conducted in 2015. DPC ⁺ (1125 mL ha ^-1) was applied after peak blooming stage on 24 July, with water as a control (CK). DPC ⁺ application significantly decreased plant height and reduced the nodes above the last white flower (NAWF) as compared with CK. After three days of DPC ⁺ treatment, cotton shoot apical meristem (SAM) became flatter than CK, and the ratio of height/length of SAM was significantly less than that of CK. With respect to redox status at shoot apex, O₂ ^-production rate, H₂O₂ generation and MDA content were significantly increased at six hours after DPC ⁺ application. In addition, the expression of GhSPL3 (a SPL transcription factor, which might play an important role in bud differentiation, the transition of growth phase and flower formation), GhV1 (a B3-domain containing transcription factor, which potentially involved in floral initiation), and GhREV3 (a class III homeodomain-leucine zipper transcription factors, which has key roles in meristem and organ development) were down-regulated by DPC ⁺ also at six hours after application. In conclusion, DPC ⁺ application during later flowering period can implement cotton chemical topping by inducing short-time oxidative stress at cotton apex, down-regulating genes involved in SAM development, flower bud differentiation, and reducing cotton shoot growth.

Key words: cotton, fortified mepiquat chloride, chemical topping, anatomy of shoot apical meristem, redox status, gene expression

Jing AN,Fang LI,Chun-Jiang ZHOU,Xiao-Li TIAN,Zhao-Hu LI. Morpho-physiological Responses of Cotton Shoot Apex to the Chemical Topping with Fortified Mepiquat Chloride[J].Acta Agronomica Sinica, 2018, 44(12): 1837-1843.

Figures/Tables 5

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基因名称 Gene name	引物序列 Primer Sequences (5'-3')	基因序列来源 Gene source
GhSPL3	F: AAGGAGGGTCCAGTACGGTG; R: CAGCCTTGGCATGGAACTCA	GenBank: KJ622311.1
GhV1	F: TGGGTGAGGGAGATGTCTGT; R: GGGCGGCATTTCATTGAGAC	GenBank: GU929695
GhREV3	F: TATTCCGGCGCTGCGCTATG; R: TGTTGCTCAAGCTCTTGCCG	Cottongen: CotAD_04603
GhACT9	F: GCCTTGGACTATGAGCAGGA; R: AAGAGATGGCTGGAAGAGGA	GenBank: AY305737.1

Morpho-physiological Responses of Cotton Shoot Apex to the Chemical Topping with Fortified Mepiquat Chloride

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