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Acta Agronomica Sinica ›› 2019, Vol. 45 ›› Issue (8): 1137-1145.doi: 10.3724/SP.J.1006.2019.84159

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Flowering genes in oilseed rape: identification, characterization, evolutionary and expression analysis

WANG Yan-Hua1,2,XIE Ling1,2,YANG Bo1,2,CAO Yan-Ru1,2,LI Jia-Na1,2,*()   

  1. 1 College of Agronomy and Biotechnology, Southwest University/Chongqing Engineering Research Center for Rapeseed, Chongqing 400715, China
    2 Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
  • Received:2018-11-16 Accepted:2019-04-15 Online:2019-08-12 Published:2019-07-16
  • Contact: Jia-Na LI E-mail:ljn1950@swu.edu.cn
  • Supported by:
    This study was supported by the Special Project of Chongqing People’s Livelihood Project(cstc2016shms-ztzx80020);the Project of Intellectual Base for Discipline Innovation in Colleges and Universities (“111” Project)(B12006)

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Abstract:

Flowering is a prerequisite for successful sexual reproduction. Controlling of flowering time is important for crop production in different geographical regions. However, few information regarding flowering genes or their evolution at genome-wide level in Brassica napus has been reported. In this study, identification, characterization, evolutionary and expression analysis of flowering genes in oilseed rape were performed. In total, 1173 flowering-related genes classified into nine types and distributed unevenly on the chromosomes were identified at the genome level of Brassica napus. Compared with Brassica rapa (AA, 2n = 20) and Brassica oleraca (CC, 2n = 18), B. napus (AACC, 2n = 38) showed significantly enlarge number of flowering-related genes due to natural hybridization and chromosome doubling. Selective pressure analysis showed that the autonomous pathway genes had less selection pressure than the genes involved in sugar metabolic pathway, suggesting that some key flowering-related genes are relatively conserved between B. napus and Arabidopsis thaliana. The present study provides more information on the B. napus flowering pathways and sheds light on the evolutionary relationship of flowering-related genes between B. napus and A. thaliana.

Key words: Brassica napus, flowering genes, evolution, regulatory pathway, expression analysis

Fig. 1

Identification and classification of flowering-related genes in B. napus Ag: aging pathway; At: ambient temperature; Au: autonomous pathway; Cp: clock and photoperiod, pathway; Fd: flower development and apical meristem response pathway; Ft: flowering time integrator; Ho: hormones pathway; Su: sugar signal; Ve: vernalization."

Fig. 2

Distribution of flowering genes on B. napus chromosomes"

Fig. 3

Diverse flowering genes sets in Arabidopsis thaliana, Brassica napus, Brassica oleracea, and Brassica rapa 缩写同图1。Abbreviation are the same as those given in Fig. 1."

Fig. 4

Direction and magnitude of natural selection acting on different flowering gene sets 缩写同图1。Abbreviations are the same as those given in Fig. 1."

Fig. 5

Heatmap of B. napus flowering gene expression profiles 缩写同图1。Abbreviations are the same as those given in Fig. 1."

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