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Acta Agronomica Sinica ›› 2019, Vol. 45 ›› Issue (10): 1455-1467.doi: 10.3724/SP.J.1006.2019.91003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Seedling and slow rusting resistance to leaf rust in 70 introduced wheat lines

ZHENG Hui-Min1,WEN Xiao-Lei1,2,HAO Chen-Yang3,ZHANG Pei-Pei1,GEBREWAHID Takele Weldu1,YAN Xiao-Cui1,LIU Da-Qun1,ZHANG Xue-Yong3,*(),LI Zai-Feng1,*()   

  1. 1College of Plant Protection, Hebei Agricultural University / Biological Control Center of Plant Disease and Plant Pests of Hebei Province, Baoding 071000, Hebei, China
    2Hebei Normal University of Science & Technology, Qinhuangdao 066000, Hebei, China
    3Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2019-01-07 Accepted:2019-05-12 Online:2019-10-12 Published:2019-09-10
  • Contact: Xue-Yong ZHANG,Zai-Feng LI E-mail:zhangxueyong@caas.cn;lzf7551@aliyun.com
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31571662);This study was supported by the National Natural Science Foundation of China(31601299);the Program of Introducing International Super Agricultural Science and Technology (948 Program)(2016-X16)

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Abstract:

Leaf rust is one of the most important wheat diseases which has a great influence on yield. Breeding cultivars for durable resistance can effectively and economically control the disease. In this study, seventy introduced wheat varieties, including susceptible control Zhengzhou 5389 and thirty-six donor lines, were inoculated with 19 Chinese pathotypes of Puccinia triticina for leaf rust resistance genes postulation at seedling stage, and to detect APR genes at adult plant stage during the 2016-2017 cropping seasons in Zhoukou of Henan province and Baoding of Hebei province. Zhengzhou 5389 and Saar lines as a susceptible and resistance control, respectively, and thirty-six tester lines with known Lr genes were also used in the present study. Based on the results of gene postulation, marker-assisted detection and pedigree analysis, fifteen Lr genes, including Lr1, Lr2a, Lr26, Lr3ka, Lr11, Lr17, Lr30, Lr10, Lr14a, Lr2b, Lr13, Lr15, Lr21, Lr44, and Lr45, were identified among the tested cultivars. Wheat cultivars with the identified resistance genes in the present study can be used for breeding resistant cultivars of wheat leaf rust in China.

Key words: introduced wheat of abroad, leaf rust, identification of genes, slow-rusting resistance

Fig. 1

Part of PCR amplification of foreign wheat cultivars with the markers Lr1, Lr9, Lr10, Lr19, Lr20, Lr24, Lr26, Lr34, and Lr37 M: DL 2000 marker; Z: Zhengzhou 5389; 1: Geneva; 2: Dodge; 3: Williams; 4: Lancer; 5: Madsen; 6: GR876; 7: Tiber; 8: Laura; 9: Marberg; 10: Karl; 11: Georgia 100; 12: Sharp; 13: Wakefield."

Fig. 2

Molecular marker detection to Lr46 gene of wheat M: PBR 322 DNA/Masp Ι marker; Z: Zhengzhou 5389; 1: Glenman; 2: Siouxland; 3: Adder; 4: Copper; 5: Century; 6: Geneva; 7: Dodge; 8: Williams; 9: Lancer; 10: Madsen; 11: GR876; 12: Tiber; 13: Laura; 14: Marberg; 15: Karl; 16: Georgia 100; 17: Sharp; 18: Wakefield; 19: Hoff; 20: Gene; 21: Vista; 22: AC Taber; 23: Alliance; 24: McNeal."

Table 1

Infection typesa of 36 reference lines inoculated with 19 Puccinia triticina （Pt） races"

Table 2

Seeding infection types of 70 varieties (lines) when tested with 19 P.triticina pathotypes"

Table 3

Analysis of variance for final disease severity of leaf rust in 70 wheat genotypes, including slow rusting cultivar Saar and susceptible check Zhengzhou 5389, tested at Baoding in Hebei province and Zhoukou in Henan province, respectively, in 2016-2017 growing seasons"

变异来源
Source of variation		 均方
MS		 F值F-value P值
P-value
品种Variety (V) 853.62 14.43** <0.0001
环境Environment (E) 9660.50 163.35** <0.0001
重复Replicate (R) 5724.50 66.80 0.0734
品种×环境V × E 218.71 3.7** <0.0001
品种×重复V × R 53.85 0.91 0.6532
误差Error 59.14

Table 4

Seedling Infection types (ITs) with mixed P. triticina (Pt) pathotype in the seedling test and final disease severity (FDS, %) in field experiments in Baoding and Zhoukou in 2016-2017 growing seasons for wheat cultivars with slow rusting resistance to leaf rust"

编号
No.		 品种
Variety		 苗期对混合
小种抗性
Seedling IT to
mix species		 2017保定
严重度1
FDS of Baoding
(%)		 2017保定
严重度2
FDS of Baoding
(%)		 2017周口
严重度1
FDS of Zhoukou
(%)		 2017周口
严重度2
FDS of Zhoukou
(%)		 均值
Average FDS
(%)
SAAR 3 5.00 10.00 1.00 15.00 7.75
5389 4 90.00 85.00 85.00 90.00 87.50
2 Satanta 4 5.00 5.00 15.00 30.00 13.75
5 TAM 105 4 5.00 1.00 35.00 20.00 15.25
6 Early Red Fife 4 5.00 25.00 15.00 25.00 17.50
10 Redman 4 1.00 30.00 1.00 1.00 8.25
12 Neepawa 4 5.00 20.00 15.00 25.00 17.50
16 Dirkwin 4 5.00 1.00 15.00 25.00 8.25
19 Stacy 4 5.00 3.00 25.00 25.00 14.50
21 Mit 4 25.00 25.00 5.00 5.00 15.00
22 Wheeler 4 35.00 15.00 10.00 10.00 17.50
编号
No.		 品种
Variety		 苗期对混合
小种抗性
Seedling IT to
mix species		 2017保定
严重度1
FDS of Baoding
(%)		 2017保定
严重度2
FDS of Baoding
(%)		 2017周口
严重度1
FDS of Zhoukou
(%)		 2017周口
严重度2
FDS of Zhoukou
(%)		 均值
Average FDS
(%)
26 Saluda 4 5.00 20.00 15.00 15.00 13.75
27 Glenman 4 5.00 15.00 20.00 20.00 15.00
30 Copper 4 5.00 5.00 15.00 15.00 10.00
31 Century 4 5.00 20.00 20.00 20.00 16.25
33 Dodge 4 5.00 5.00 25.00 30.00 16.25
34 Williams 4 1.00 15.00 20.00 20.00 14.00
35 Lancer 4 5.00 5.00 15.00 25.00 12.50
36 Madsen 4 5.00 15.00 20.00 20.00 15.00
37 GR876 4 5.00 15.00 15.00 35.00 17.50
38 Tiber 4 1.00 15.00 10.00 30.00 14.00
39 Laura 4 1.00 1.00 30.00 30.00 15.50
41 Karl 4 5.00 5.00 15.00 35.00 15.00
43 Sharp 4 5.00 5.00 30.00 30.00 17.50
44 Wakefield 4 5.00 5.00 15.00 35.00 15.00
45 Hoff 4 5.00 20.00 20.00 20.00 16.25
46 Gene 4 1.00 1.00 1.00 20.00 5.75
47 Vista 4 1.00 20.00 5.00 30.00 14.00
48 AC Taber 4 1.00 20.00 10.00 25.00 14.00
50 McNeal 4 5.00 25.00 10.00 30.00 17.50
52 Lambert 4 10.00 10.00 20.00 20.00 15.00
53 Pomerelle 4 5.00 5.00 15.00 45.00 17.50
54 Cayuga 4 5.00 25.00 20.00 20.00 17.50
55 Boundary 4 10.00 10.00 15.00 25.00 15.00
56 Caledonia 4 5.00 30.00 15.00 15.00 16.25
57 Millennium 4 5.00 5.00 1.00 35.00 11.50
58 Jubilee 4 5.00 30.00 20.00 20.00 18.75
60 Avalanche 4 5.00 25.00 10.00 5.00 11.25
65 Alson 4 1.00 20.00 15.00 15.00 12.75
66 Steel-ND 4 15.00 15.00 20.00 10.00 15.00
68 Grandin 4 10.00 5.00 20.00 35.00 17.50
LSD 10.84
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