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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (02): 249-258.doi: 10.3724/SP.J.1006.2020.94078

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Increasing phosphate fertilizer application to improve photosynthetic capacity and yield of summer soybean in weak light environment

ZHAO Wei1,ZHEN Tian-Yue1,ZHANG Zi-Shan2,XU Zheng1,GAO Da-Peng1,DING Cong1,LIU Peng1,LI Geng1,*(),NING Tang-Yuan1,*()   

  1. 1 Agronomy College, Shandong Agricultural University / State Key Laboratory of Crop Biology, Tai’an 271018, Shandong, China
    2 Life Science College, Shandong Agricultural University, Tai’an 271018, Shandong, China
  • Received:2019-05-22 Accepted:2019-08-09 Online:2020-02-12 Published:2019-09-11
  • Contact: Geng LI,Tang-Yuan NING E-mail:ligeng213@sina.com;ningty@163.com
  • Supported by:
    This study was supported by the National Key Research and Development Program of China National(2016YFD0300205);the National Natural Science Foundation of China(31401339);the China Special Fund for Agro-scientific Research in the Public Interest(201503130);the China Special Fund for Agro-scientific Research in the Public Interest(201503121)

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Abstract:

In order to study the effect of phosphate fertilizer application on the photosynthetic characteristics of summer soybean in weak light environment, two light treatments [normal light (L1) and weak light (L2)] with three phosphate fertilizer treatments including non-phosphate fertilizer application (P0), conventional phosphate fertilizer application (P1), and excessive-phosphate fertilizer application (P2) in each light treatment were set up to measure the gas exchange, chlorophyll a fluorescence differences of photosynthetic performance as well as the yield and its components in Qihuang 34. The yield reduced significantly in weak light treatment, with an average of 61.4% in two years lower than that under the normal light. The 2-year average yield of P2 was 8.4% and 3.2% higher than that of P0 and P1 respectively under the normal light, but 21.7% and 12.2% higher than P0 and P1 in weak light treatment respectively, indicating the effect of excessive-phosphate fertilizer on yield was more pronounced under weak light. The weak light environment significantly increased the leaf area, specific leaf area, chlorophyll a and chlorophyll b contents, which was enhanced by increasing phosphate fertilizer application. The net photosynthetic rate and stomatal conductance of the leaf decreased significantly in the weak light environment, while the intercellular CO2 concentration increased, indicating the reduction of carbon assimilation in weak light environment was not limited by stomata. Increasing the application of phosphate fertilizer increased photosynthetic rate and stomatal conductance, which was more obvious under weak light. Excessive-phosphate fertilizer application reduced the relative fluorescence at the K and J points of the OJIP curve, and improved the electron transfer performance of photosystem II, was more significant which in weak light than in normal light environment. Therefore, the increase of photosynthetic electron transport activity effectively alleviates the decrease of leaf photosynthetic rate under weak light treatment, which may be the reason for the significant increase of dry matter accumulation and yield by applying more phosphate fertilizer in weak light environment.

Key words: soybean, weak light environment, phosphate fertilizer, photosynthetic capacity, yield

Table 1

Effects of different phosphate fertilizer application and light environment treatments on yield and its components of summer soybean"

年份
Year		 处理Treatment 百粒重
100-seed weight (g)		 单株有效荚数
Effective pods per plant		 单株粒数
Seeds per plant		 产量
Yield (kg hm-2)
光照 Light 磷肥 Phosphorous
2017 L1 P0 28.08 c 50.00 c 116.30 b 3918.28 c
P1 28.26 c 54.70 b 120.73 a 4094.59 b
P2 28.42 bc 59.80 a 124.06 a 4231.07 a
L2 P0 29.41 ab 29.60 f 66.09 e 2332.27 f
P1 29.81 a 35.00 e 71.16 d 2545.21 e
P2 30.03 a 39.10 d 78.12 c 2815.48 d
变异来源 Source of variation 方差分析 Analysis of variance
光照 Light (L) ** ** ** **
磷肥 Phosphorous (P) NS ** ** **
光照×氮肥 (L×P) NS NS * *
2018 L1 P0 28.16 a 51.88 b 118.03 b 3988.97 c
P1 28.48 a 52.25 b 123.45 a 4218.65 b
P2 28.62 a 54.11 a 126.48 a 4344.13 a
L2 P0 29.00 a 31.75 d 66.65 e 2319.62 f
P1 29.07 a 35.64 c 71.70 d 2500.61 e
P2 29.21 a 34.48 c 81.25 c 2847.66 d
变异来源 Source of variation 方差分析 Analysis of variance
光照 Light (L) NS ** ** **
磷肥 Phosphorous (P) NS ** ** **
光照×氮肥 (L×P) NS * * *

Fig. 1

Effects of shade on photosynthetic active radiation of soybean canopy L1: natural light environment; L2: low light environment with light transmittance of (60±5)%."

Fig. 2

Effects of different phosphate fertilizer application and light environment treatments on soybean leaf area after anthesis Different letters indicate significant differences among treatments at the 0.05 probability level. R1: beginning flowering; R3: beginning pod; R5: beginning seed; R7: beginning maturity; LA: leaf area per plant; SLA: specific leaf area. Treatments are the same as those given in Table 1."

Fig. 3

Effects of different phosphate fertilizer application and light environment treatments on chlorophyll content of soybean leaf after anthesis Different letters indicate significant differences among treatments at the 0.05 probability level. R1: beginning of flowering; R3: beginning of podding; R5: beginning of grain filling. Treatments are the same as those given in Table 1."

Fig. 4

Effects of different phosphate fertilizer application and light environment treatments on gas exchange parameters of soybean leaf after anthesis Different letters indicate significant differences among treatments at the 0.05 probability level. R1: beginning of flowering; R3: beginning of podding; R5: beginning of grain filling; R7: beginning of maturity. Treatments are the same as those given in Table 1."

Fig. 5

Effects of different phosphate fertilizer application and light environment treatments on Wk, Vj and their correlation in soybean leaf after anthesis Different letters indicate significant differences among treatments at the 0.05 probability level. R1: beginning of flowering; R3: beginning of podding; R5: beginning of grain filling; R7: beginning of maturity. Treatments are the same as those given in Table 1."

Fig. 6

Effects of different phosphate fertilizer application and light environment treatments on reaction center performance of photosystem II and their correlation in soybean leaf after anthesis Different letters indicate significant differences among treatments at the 0.05 probability level. R1: beginning of flowering; R3: beginning of podding; R5: beginning of grain filling; R7: beginning of maturity. Treatments are the same as those given in Table 1."

[1] Li Y, Xin G F, Wei M, Shi Q H, Yang F J, Wang X F . Carbohydrate accumulation and sucrose metabolism responses in tomato seedling leaves when subjected to different light qualities. Sci Hortic, 2017,225:490-497.
doi: 10.1016/j.scienta.2017.07.053
[2] Kaiser E, Weerheim K, Schipper R, Dieleman J A . Partial replacement of red and blue by green light increases biomass and yield in tomato. Sci Hortic, 2019,249:271-279.
doi: 10.1016/j.scienta.2019.02.005
[3] 任国玉, 郭军, 徐铭志, 初子莹, 张莉, 邹旭恺, 李庆祥, 刘小宁 . 近50年中国地面气候变化基本特征. 气象学报, 2005,63:942-956.
doi: 10.11676/qxxb2005.090
Ren G Y, Guo J, Xu M Z, Chu Z Y, Zhang L, Zou X K, Li Q X, Liu X N . Climate changes of China’s mainland over the past half century. Acta Meteor Sin, 2005,63:942-956 (in Chinese with English abstract).
doi: 10.11676/qxxb2005.090
[4] 崔海岩, 靳立斌, 李波, 赵斌, 董树亭, 刘鹏, 张吉旺 . 大田遮阴对夏玉米光合特性和叶黄素循环的影响. 作物学报, 2013,39:478-485.
doi: 10.3724/SP.J.1006.2013.00478
Cui H Y, Jin L B, Li B, Zhao B, Dong S T, Liu P, Zhang J W . Effects of shading on photosynthetic characteristics and xanthophyll cycle of summer maize in the field. Acta Agron Sin, 2013,39:478-485 (in Chinese with English abstract).
doi: 10.3724/SP.J.1006.2013.00478
[5] Valladares F, Niinemets U . Shade tolerance, a key plant feature of complex nature and consequences. Annu Rev Ecol Evol Syst, 2008,39:237-257.
doi: 10.1146/annurev.ecolsys.39.110707.173506
[6] Wu Y S, Yang F, Gong W Z, Shoaib A, Fan Y F, Wu X L, Yong T W, Liu W G, Shu K, Liu J, Du J B, Yang W Y . Shade adaptive response and yield analysis of different soybean genotypes in relay intercropping systems. J Integr Agric, 2017,16:1331-1340.
doi: 10.1016/S2095-3119(16)61525-3
[7] Yang X Q, Zhang Q S, Zhang D, Sheng Z T . Light intensity dependent photosynthetic electron transport in eelgrass (Zostera marina L.). Plant Physiol Biochem, 2017,113:168-176.
doi: 10.1016/j.plaphy.2017.02.011 pmid: 28236752
[8] Huang W, Zhang S B, Liu T . Moderate photoinhibition of photosystem II significantly affects linear electron flow in the shade-demanding plant Panax notoginseng. Front Plant Sci, 2018,9:250-256.
doi: 10.3389/fpls.2018.00250 pmid: 29599786
[9] Sun J L, Sui X L, Huang H Y, Wang S H, Wei Y X, Zhang Z X . Low light Stress down-regulated Rubisco gene expression and photosynthetic capacity during cucumber (Cucumis sativus L.) leaf development. J Integr Agric, 2014,13:997-1007.
doi: 10.1016/S2095-3119(13)60670-X
[10] Hussain S, Iqbal N, Brestic M, Raza M A, Pang T, Langham D R, Safdar M E, Ahmed S, Wen B X, Gao Y, Liu W G, Yang W Y . Changes in morphology, chlorophyll fluorescence performance and Rubisco activity of soybean in response to foliar application of ionic titanium under normal light and shade environment. Sci Total Environ, 2019,658:626-637.
doi: 10.1016/j.scitotenv.2018.12.182 pmid: 30580217
[11] 任永福, 陈国鹏, 蒲甜, 陈诚, 曾瑾汐, 彭霄, 马艳玮, 杨文钰, 王小春 . 玉米-大豆带状种植中套作高光效玉米窄行穂位叶光合特性对弱光胁迫的响应. 作物学报, 2019,45:728-739.
doi: 10.3724/SP.J.1006.2019.83040
Ren Y F, Chen G P, Pu T, Chen C, Zeng J X, Peng X, Ma Y W, Yang W Y, Wang X C . Responses of photosynthetic characteristics to low light stress in ear leaves of high photosynthetic efficiency maize at narrow row of maize-soybean strip intercropping system. Acta Agron Sin, 2019,45:728-739 (in Chinese with English abstract).
doi: 10.3724/SP.J.1006.2019.83040
[12] 孙映波, 于波, 黄丽丽, 周彤彤, 赵超艺, 张佩霞 . 不同栽培环境对耐冬山茶生长及荧光参数的影响. 热带作物学报, 2018,39:1553-1560.
Sun Y B, Yu B, Huang L L, Zhou T T, Zhao C Y, Zhang P X . Effects of different cultivation environment on the growth and fluorescence parameters of Camellia japonica L. Chin J Trop Crops, 2018,39:1553-1560 (in Chinese with English abstract).
[13] 朱文美, 费立伟, 代兴龙, 张秀, 董述鑫, 初金鹏, 钤太峰, 贺明荣 . 雨养和灌水条件下种植密度对冬小麦产量、氮素利用率和水分利用效率的影响. 山东农业科学, 2018,50(8):35-41.
Zhu W M, Fei L W, Dai X L, Zhang X, Dong S X, Chu J P, Qian T F, He M R . Effects of planting density on grain yield, nitrogen and water use efficiency of winter wheat in rainfed and irrigation regimes. Shandong Agric Sci, 2018,50(8):35-41 (in Chinese with English abstract).
[14] 张明聪, 何松榆, 金喜军, 王孟雪, 任春元, 战英策, 胡国华, 张玉先 . 氮磷调控对大豆-玉米轮作下植株光合生产能力和产量的影响. 大豆科学, 2018,37:883-890.
Zhang M C, He S Y, Jin X J, Wang M X, Ren C Y, Zhan Y C, Hu G H, Zhang Y X . Effects of nitrogen and phosphorus regulation on photosynthetic capacity and yield under soybean and maize rotation. Soybean Sci, 2018,37:883-890 (in Chinese with English abstract).
[15] Yao H S, Zhang Y L, Yi X P, Zhang X J, Zhang W F . Cotton responds to different plant population densities by adjusting specific leaf area to optimize canopy photosynthetic use efficiency of light and nitrogen. Field Crops Res, 2016,188:10-16.
doi: 10.1016/j.fcr.2016.01.012
[16] Kutamal A S, Aliyul B S, Saratu A . Influence of phosphorus fertilizer on the development of root nodules in cowpea (Vigna unguiculata L. Walp) and soybean(Glycine max L. Merrill). Int J Pure Appl Sci, 2008,2:27-31.
[17] 王菲, 曹翠玲 . 磷水平对不同磷效率小麦叶绿素荧光参数的影响. 植物营养与肥料学报, 2010,16:758-762.
doi: 10.11674/zwyf.2010.0335
Wang F, Cao C L . Effects of phosphorus levels on chlorophyll fluorescence parameters of wheat (Triticum aestivum L.) with different phosphorus efficiencies. Plant Nutr Fert Sci, 2010,16:758-762 (in Chinese with English abstract).
doi: 10.11674/zwyf.2010.0335
[18] 焦念元, 杨萌珂, 宁堂原, 尹飞, 徐国伟, 付占国, 李友军 . 玉米花生间作和磷肥对间作花生光合特性及产量的影响. 植物生态学报, 2013,37:1010-1017.
doi: 10.3724/SP.J.1258.2013.00104
Jiao N Y, Yang M K, Ning T Y, Yin F, Xu G W, Fu Z G, Li Y J . Effects of maize-peanut intercropping and phosphate fertilizer on photosynthetic characteristics and yield of intercropped peanut plants. Chin J Plant Ecol, 2013,37:1010-1017 (in Chinese with English abstract).
doi: 10.3724/SP.J.1258.2013.00104
[19] Janik E, Bednarska J, Zubik M, Luchowski R, Mazur R, Sowinski K, Grudzinski W, Garstka M, Gruszecki W I . A chloroplast “wake up” mechanism: Illumination with weak light activates the photosynthetic antenna function in dark-adapted plants. J Plant Physiol, 2017,210:1-8.
doi: 10.1016/j.jplph.2016.12.006 pmid: 28040624
[20] Jiao Y, Ouyang H L, Jiang Y J, Kong X Z, He W, Liu W X, Yang B, Xu F L . Effects of phosphorus stress on the photosynthetic and physiological characteristics ofChlorella vulgaris based on chlorophyll fluorescence and flow cytometric analysis. Ecol Indic, 2017,78:131-141.
doi: 10.1016/j.ecolind.2017.03.010
[21] Zhang W, Chen X X, Liu Y M, Liu D Y, Du Y F, Chen X P, Zou C Q . The role of phosphorus supply in maximizing the leaf area, photosynthetic rate, coordinated to grain yield of summer maize. Field Crops Res, 2018,219:113-119.
doi: 10.1016/j.fcr.2018.01.031
[22] Lichtenthaler H K, Wellburn A R . Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Anal Peach, 1983,11:591-592.
doi: 10.1161/01.hyp.11.6.591 pmid: 2839415
[23] Yu J, Wang M J, Dong C, Xie B Z, Liu G H, Fu Y M, Liu H . Analysis and evaluation of strawberry growth, photosynthetic characteristics, biomass yield and quality in an artificial closed ecosystem. Sci Hortic, 2015,195:188-194.
doi: 10.1016/j.scienta.2015.09.009
[24] Alaka Srivastava G, Strasser R J . Characterization of the 820-nm transmission signal paralleling the chlorophyll a fluorescence rise (OJIP) in pea leaves. Funct Plant Biol, 2003,30:785-796.
doi: 10.1071/FP03032
[25] Strasser R J, Tsimill-Michael M, Srivastava A . Analysis of the chlorophyll a fluorescence transient. Photosynthesis, 2004,12:1-47.
doi: 10.1016/j.saa.2018.09.036 pmid: 30282060
[26] 韩霜, 陈发棣 . 植物对弱光的响应研究进展. 植物生理学报, 2013,49:309-316.
Han S, Chen F D . Research progress in plant response to weak light. Plant Physiol J, 2013,49:309-316 (in Chinese with English abstract).
[27] Plénet D, Etchebest S, Mollier A, Pellerin S . Growth analysis of maize field crops under phosphorus deficiency. Plant Soil, 2000,223:119-132.
doi: 10.1023/A:1004877111238
[28] Usuda H, Shimogawara K . Phosphate deficiency in maize: I. Leaf phosphate status, growth, photosynthesis and carbon partitioning. Plant Cell Physiol, 1991,32:497-504.
[29] Kirschbaum M U F, Tompkins D . Photosynthetic responses to phosphorus nutrition inEucalyptus grandis seedlings. Aust J Plant Physiol, 1990,17:527-535.
doi: 10.1155/2019/9058715 pmid: 31534966
[30] Sejima T, Takagi D, Fukayama H, Makino A, Miyake C . Repetitive short-pulse light mainly inactivates photosystem I in sunflower leaves. Plant Cell Physiol, 2014,55:1184-1193.
doi: 10.1093/pcp/pcu061
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