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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (8): 1208-1216.doi: 10.3724/SP.J.1006.2020.94183

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification and selection for anti-browning potato varieties (lines)

CHEN Ming-Jun1,SHU Qi-Qiong3,XU Jian-Fei2,LUO Xiao-Bo1,LEI Zun-Guo1,JIN Li-Ping2,*(),LI Fei1,*()   

  1. 1Institute of Potato, Guizhou Academy of Agricultural Sciences, Guiyang 550006, Guizhou, China
    2Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    3College of Life Sciences, Guizhou Normal University, Guiyang 550000, Guizhou, China
  • Received:2019-11-25 Accepted:2020-03-24 Online:2020-08-12 Published:2020-04-26
  • Contact: Li-Ping JIN,Fei LI E-mail:jinliping@caas.cn;gzlfei@sina.com
  • Supported by:
    Sub-Project of the National Key Research and Development Plan (Cultivation of New Potato Varieties with High Quality, Multi Resistance and Strong Adaptability of China)(2017YFD0101905);Modern Agriculture International Science and Technology Cooperation Base of Guizhou (the Science and Technology Cooperation Talent Platform of Guizhou [2019] 5804)

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Abstract:

Potato tuber is easy to be damaged and caused browning, which will affect its nutrition, sense quality and safety. Different potato varieties have different resistances to browning. In this study, 27 different potato germplasm resources,were used to measure four indexes including polyphenol oxidase (PPO), browning degree (BD), browning index and after cooking darkening (ACD), for indentifying and selecting anti-browning potato varieties (lines). The 27 materials could be divided into four groups. High anti-browning, group including 09306-82 and 13041-52 had high resistance to browning. Anti-browning group, included Yanshu 4, Q 8, 12-1, Chunshu 3, 19-1, 14018-142, Atlantic, Longshu 12, Weiyu 5, Lishu 6, Chunshu 5, and Zaodabai, had some obvious deficiencies in the related anti-browning indicators. Medium anti-browning group, including 09001-136, Zhongshu 5, Longshu 4, Dongnong 310, Qingshu 9, 15-1, Yunshu 505, BF006, Minshu 1, and Qianyu 8, had poor resistance to browning. Easy browning group, containing Chunshu 6 and Favorita, had very weak resistance to browning. The selected varieties (lines) with very high or high resistance to browning can be used in potato breeding for browning resistance , providing variety support for potato processing industry, and the easy browning materials can be used to study the browning mechanism of potato.

Key words: potato, anti-browning, PPO, browning index, browning degree, after cooking darkening

Table 1

Main agronomic traits of potato varieties (lines)"

编号
No.		 品种(系)
Variety (line)		 株高
Plant height (cm)		 花色
Flower color		 薯形
Tuber shape		 主茎数
Main stem number		 肉色
Flesh color		 芽眼深度
Eye depth
1 大西洋Atlantic 62 淡紫色Lilac 卵圆形Oval 3 白色White 浅Shallow
2 黔芋8号Qianyu 8 60 白色White 椭圆形Oblong 8 黄色Yellow 浅Shallow
3 中薯5号Zhongshu 5 61 白色White 扁圆形Oblateness 4 淡黄Flaxen 浅Shallow
4 闽薯1号Minshu 1 43 白色White 长圆形Obround 4 淡黄Flaxen 浅Shallow
5 09306-82 62 紫色Purple 长圆形Obround 5 黄色Yellow 浅Shallow
6 09001-136 65 白色White 卵圆形Oval 4 黄色Yellow 浅Shallow
7 费乌瑞它Favorita 48 蓝紫色
Bluish violet		 椭圆形Oblong 4 黄色Yellow 浅Shallow
8 龙薯6号 Longshu 6 49 淡紫色Lilac 椭圆形Oblong 4 黄色Yellow 浅Shallow
9 龙薯12号 Longshu 12 51 淡紫色Lilac 扁圆形Oblateness 3 白色White 浅Shallow
10 19-1 48 白色White 椭圆形Oblong 3 黄色Yellow 中Medium
11 延薯4号Yanshu 4 49 白色White 圆形Roundness 3 黄色Yellow 中Medium
12 春薯6号Chunshu 6 54 白色White 椭圆形Oblong 5 黄色Yellow 浅Shallow
13 12-1 66 白色White 椭圆形Oblong 4 黄色Yellow 浅Shallow
14 14018-142 57 紫色Purple 椭圆形Oblong 6 黄色Yellow 中Medium
15 威芋5号Weiyu 5 61 白色White 椭圆形Oblong 5 黄色Yellow 浅Shallow
16 东农310 Dongnong 310 60 淡紫色Lilac 扁圆形Oblateness 4 白色White 浅Shallow
17 春薯3号Chunshu 3 55 白色White 圆形Roundness 4 白色White 浅Shallow
18 龙薯4号Longshu 4 47 紫色Purple 长圆形Obround 4 黄色Yellow 浅Shallow
19 13041-52 42 白色White 卵圆形Oval 5 淡黄Flaxen 浅Shallow
20 云薯505 Yunshu 505 59 白色White 扁圆形Oblateness 7 白色White 浅Shallow
21 BF006 64 白色White 长圆形Obround 7 黄色Yellow 浅Shallow
22 15-1 50 紫色Purple 椭圆形Oblong 6 黄色Yellow 浅Shallow
23 早大白Zaodabai 64 白色White 长圆形Obround 2 白色White 浅Shallow
24 春薯5号Chunshu 5 60 白色White 扁圆形Oblateness 5 白色White 浅Shallow
25 Q8 51 淡紫色Lilac 椭圆形Oblong 5 黄色Yellow 浅Shallow
26 青薯9号Qingshu 9 65 浅红色Light red 椭圆形Oblong 3 黄色Yellow 浅Shallow
27 丽薯6号Lishu 6 62 白色White 椭圆形Oblong 5 白色White 浅Shallow

Fig. 1

PPO activity of potato varieties (lines) The error line was the standard deviation of three repeated values."

Fig. 2

Browning degree of potato varieties (lines) The error line was the standard deviation of three repeated values."

Table 2

Primary selection of browning degree in anti-browning potato varieties (lines)"

编号
No.		 品种(系)
Variety (line)		 褐变强度 Browning degree
30℃/20 min 4℃/24 h 变化 Difference
5 09306-82 0.106±0.0040 0.150±0.0015 0.044±0.0055
2 黔芋8号 Qianyu 8 0.123±0.0023 0.173±0.0006 0.050±0.0015
6 09001-136 0.099±0.0059 0.158±0.0015 0.059±0.0076
1 大西洋 Atlantic 0.104±0.0078 0.182±0.0012 0.078±0.0087
7 费乌瑞它 Favorita 0.113±0.0057 0.197±0.0006 0.084±0.0090
20 云薯505 Yunshu 505 0.119±0.0032 0.205±0.0025 0.086±0.0021
3 中薯5号 Zhongshu 5 0.127±0.0031 0.217±0.0023 0.090±0.0023
14 14018-142 0.102±0.0123 0.194±0.0123 0.092±0.0050
17 春薯3号 Chunshu 3 0.120±0.0062 0.269±0.0012 0.149±0.0012
19 13041-52 0.110±0.0020 0.261±0.0021 0.151±0.0015
9 龙薯12号 Longshu 12 0.142±0.0038 0.308±0.0058 0.166±0.0021

Fig. 3

Browning phenotype of slices of potato varieties (lines) Numbers of materials and the same as those given in Table 1."

Fig. 4

Browning index of slices of potato varieties (lines)"

Table 3

Browning index in anti-browning potato varieties (lines)"

编号
No.		 品种(系)
Variety (line)		 初始褐变时间
Initial browning time (h)		 褐变指数 Browning index (%)
3 h 6 h 8 h 12 h 24 h 平均
Average
5 09306-82 6 5 15 15 15 15 13
11 延薯4号 Yanshu 4 8 10 10 10 25 40 19
19 13041-52 12 5 5 5 10 75 20
13 12-1 6 15 15 25 25 25 21
10 19-1 4 15 15 15 25 40 22
17 春薯3号 Chunshu 3 8 10 10 20 15 55 22
25 Q8 12 15 15 15 5 70 24
14 14018-142 5 10 15 15 35 55 26
1 大西洋 Atlantic 6 10 40 45 35 20 30
21 BF006 6 10 25 25 25 65 30
27 丽薯6号 Lishu 6 12 15 25 25 20 65 30
24 春薯5号 Chunshu 5 1 15 15 15 45 95 37
9 龙薯12号 Longshu 12 6 10 55 55 55 55 46
15 威芋5号 Weiyu 5 3 35 35 40 40 85 47
23 早大白 Zaodabai 1 35 45 45 40 70 47

Fig. 5

Browning of potato varieties (lines) after cooking Numbers of materials and the same as those given in Table 1."

Fig. 6

Browning grading of potato varieties (lines) after cooking"

[1] 胡军, 段绍光, 徐建飞, 卞春松, 李广存, 庞万福, 金黎平. 马铃薯块茎褐变相关研究进展. 见: 屈冬玉, 陈伊里主编. 马铃薯产业与脱贫攻坚(2018). 哈尔滨: 哈尔滨地图出版社, 2018. pp 119-123.
Hu J, Duan S G, Xu J F, Bian C S, Li G C, Pang W F, Jin L P. Research progress of potato tuber browning. In: Qu D Y, Chen Y L, eds. Potato Industry and Poverty Alleviation (2018). Harbin: Harbin Map Press, 2018. pp 119-123(in Chinese).
[2] Shepherd L V T, Alexander C J, Hackett C A, McRae D, Sungurtas J A, Verrall S R, Morris J A, Hedley P E, Rockhold D, Belknap W, Davies H V. Impacts on the metabolome of down-regulating polyphenol oxidase in potato tubers. Trans Res, 2015,24:447-461.
[3] Chang Y L, Kagan L V, Antoni W J, Susan K B. Enzymatic browning in relation to phenolic compounds and polyphenoloxidase activity among various peach cultivars. J Agric Food Chem, 1990,38:99-101.
[4] Mcevily A J, Iyengar R, Otwell W S. Inhibition of enzymatic browning in foods and beverages. Crit Rev Food Sci, 1992,32:253-273.
doi: 10.1080/10408399209527599
[5] Keilin D, Mann T. Polyphenol oxidase purification, nature and properties. Proc Royal Soc London Series B, Biol Sci, 1938,125:187-204.
[6] Capitani C D, Carvalho A C L, Botelho P B, Carrapeiro M M, Castro I A. Synergism on antioxidant activity between natural compounds optimized by response surface methodology. Eur J Lipid Sci Technol, 2009,111:1100-1110.
[7] Taranto F, Pasqualone A, Mangini G, Tripodi P, Miazzi M M, Pavan S, Montemurro C. Polyphenol oxidases in crops: biochemical, physiological and genetic aspects. Int J Mol Sci, 2017,18:377.
[8] 赵欣, 周婧, 陈湘宁, 许丽, 杨肖飞, 刘建新. OPP/CPP 膜中不同气体比例对鲜切马铃薯片保鲜的影响. 食品工业科技, 2017,38(17):207-211.
Zhao X, Zhou J, Chen X N, Xu L, Yang X F, Liu J X. Effect of different gas compositions in the OPP/CPP film on preservation of fresh-cut potato chips. Sci Technol Food Ind, 2017,38(17):207-211 (in Chinese with English abstract).
[9] Shahidi F, Ambigaipalan P. Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects. J Funct Foods, 2015,18:820-897.
[10] 苏霞, 吴厚玖. 橙汁非酶褐变机制及控制措施. 食品与发酵工业, 2011,37(7):148-151.
Su X, Wu H J. Non-enzymatic Browning of orange juice and its control measures. Food Ferment Ind, 2011,37(7):148-151 (in Chinese).
[11] Mishra B B, Gautam S, Sharma A. Browning of fresh-cut eggplant: impact of cutting and storage. Postharvest Biol Technol, 2012,67:44-51.
[12] 姜莉, 林长彬, 张明兰, 张洪福, 徐怀德. 超高压对马铃薯多酚氧化酶和过氧化物酶的钝化研究. 食品工业, 2013, (1):141-143.
Jiang L, Lin C B, Zhang M L, Zhang H F, Xu H D. Study on deactivation of ultra-high voltage to the potato polyphenoloxidase and the peroxide enzyme. Food Ind, 2013, (1):141-143 (in Chinese with English abstract).
[13] Siddiq M, Sinha N K, Cash J N. Characterization of polyphenol oxidase from Stanley plums. J Food Sci, 1992,57:1177-1179.
[14] Waltz E. USDA approves next-generation GM potato. Nat Biotechnol, 2015,33:12-13.
pmid: 25574623
[15] Murata M, Nishimura M, Murai N, Haruta M, Homma S, Itoh Y. A transgenic apple callus showing reduced polyphenol oxidase activity and lower browning potential. Biosci Biotechnol Biochem, 2001,65:383-388.
pmid: 11302173
[16] Ali H M, El-Gizawy A M, El-Bassiouny R E, Saleh M A. The role of various amino acids in enzymatic browning process in potato tubers, and identifying the browning products. Food Chem, 2016,192:879-885.
doi: 10.1016/j.foodchem.2015.07.100 pmid: 26304424
[17] Cantos E, Tudela J A, Gil M I, Espinet J C. Phenolic compounds and related enzymes are not rate-limiting in browning development of fresh-cut potatoes. J Agric Food Chem, 2002,50:3015-3023.
doi: 10.1021/jf0116350 pmid: 11982435
[18] Cabezas-Serrano A B, Amodio M L, Cornacchia R, Rinaldi R, Colelli G. Suitability of five different potato cultivars ( Solanum tuberosum L.) to be processed as fresh-cut products. Postharvest Biol Technol, 2009,53:138-144.
doi: 10.1016/j.postharvbio.2009.03.009
[19] 庞学群, 张昭其. 防褐处理对切分马蹄、马铃薯低温贮藏期间褐变的影响. 食品科学, 2002,23(4):126-129.
Pang X Q, Zhang Z Q. Effects of anti-browning treatments on Browning of split-hoof and potato during low temperature storage. Food Sci, 2002,23(4):126-129 (in Chinese with English abstract).
[20] 李山云, 隋启君, 白建明, 杨琼芬, 李文鹏. 抗机械损伤褐变马铃薯品种(系)的筛选. 中国马铃薯, 2010,24(4):193-196.
Li S Y, Sui Q J, Bai J M, Yang Q F, Li W P. Screening of potato ( Solanum tuberosum L.) cultivars (lines) for browning tolerance responding to mechanical stress. Chin Potato J, 2010,24(4):193-196 (in Chinese with English abstract).
[21] 王清, 黄惠英, 马文芳, 王蒂. 反义PPO基因对马铃薯块茎褐化的影响. 作物学报, 2007,33:1822-1827.
Wang Q, Huang H Y, Ma W F, Wang D. Effect of anti-sense PPO gene on the tuber-browning of Solanum tuberosum L. Acta Agron Sin, 2007,33:1822-1827 (in Chinese with English abstract).
[22] 王唯俊. 荷兰马铃薯高世代无性系产量及品质分析. 东北农业大学硕士学位论文, 黑龙江哈尔滨, 2011.
Wang W J. Analysis of Agronomic and Quality Traits in Advanced Dutch Potato Clones. MS Thesis of Northeast Agricultural University, Harbin, Heilongjiang, China, 2011 (in Chinese with English abstract).
[23] 王丽, 王万兴, 索海翠, 胡新喜, 秦玉芝, 曾璐, 李小波, 熊兴耀. 马铃薯块茎酶促褐变及与相关生理指标的关系. 园艺学报, 2019,46:1519-1530.
Wang L, Wang W X, Suo H C, Hu X X, Qin Y Z, Zeng L, Li X B, Xiong X Y. The relationship between enzymatic browning and relevant physiological index of potato tubers. Acta Hortic Sin, 2019,46:1519-1530 (in Chinese with English abstract).
[24] 王海艳, 王立春, 李凤云, 田国奎, 娄树宝, 李成君, 郝智勇. 马铃薯抗褐变种质资源的鉴定与筛选. 植物遗传资源学报, 2018,19:263-270.
Wang H Y, Wang L C, Li F Y, Tian G K, Lou S B, Li C J, Hao Z Y. Identification and selection for potato anti-browning germplasm resources. J Plant Genetic Resour, 2018,19:263-270 (in Chinese with English abstract).
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