Table 1

Ten candidate target genes predicted from the sequencing of novel-miR311 degradation group"

Fig. 1

Validation of target genes using 5°-RACE “:” represents the miRNA and its targets are labeled on the right, “.” represents mismatch, the arrow indicates the cleavage site, and the numbers above sequences represent the detected cleavage site of independent clones."

Fig. 2

Comparison of target gene sites between Brassica napus and Arabidopsis a: transduction protein gene; b: heat shock protein HSP70-2 gene; c: heat shock cognate protein HSC70-1 gene. The red part is the binding site of novel-miR311 and target gene."

Fig. 3

Expression levels of novel-miR311 and HSC70-1 in root, stem, leaf, flower, and pod of wild type and positive Arabidopsis thaliana Data are shown as mean ± SD of three biological replicates. * and ** represent P < 0.05 and P < 0.01, respectively."

Fig. 4

Response to high temperature stress on 1/2 MS in wild type and positive Arabidopsis thaliana a: phenotypic analysis; b: statistical analysis."

Fig. 5

Germination experiment under high temperature stress in Brassica napus positive seedlings and J572 seeds a: comparison chart before and after treatment at different temperatures; b: germination rate chart for two days."

Fig. 6

Phenotype and expression analysis under high temperature stress treatment of Brassica napus positive seedlings and J572 in soil a: comparison of positive seedlings and J572 before and after heat stress, the red arrow in figures refers to the surviving rapeseed. b: expression of novel-miR311 and HSC70-1 before and after heat stress. Data are shown as mean ± SD of three biological replicates. ** P < 0.01."

Fig. 7

HSP70 action pathway According to Jacob et al.[33] with slight modification, the results in the dashed box are for the introduction of this article."

Supplementary Fig. 1

novel-miR311 stem-loop structure"

Supplementary table 1

novel-miR311 precursor sequence and mature sequence"