In order to determine the suitable nitrogen level and planting density for single seed precision sowing of peanut, field comparison experiments were conducted using Huayu 22 with three nitrogen levels at 0 (N0), 60 (N1), 120 (N2) kg hm-2 and three planting densities at 79,300 (D1), 158,600 (D2), and 237,900 (D3) plants hm-2 in 2018 and 2019. The effects of nitrogen, density and their interaction on root morphology, plant characteristics and yields of single seed precision sowing peanut were studied by the split plot design for two factors. Nitrogen fertilizer had no significant effect on root length, root surface area, root volume and root dry weight, whereas significant on density. Root length, root surface area, root volume and dry weight per plant decreased with the increase of density, D1 was significantly higher than D2 and D3, but there was no significant difference between D2 and D3 treatments. And root length, root surface area, root volume and dry weight of unit area increased with the increase of density, D1 was significantly lower than D2 and D3, and there was no significant difference between D2 and D3 treatments. The interaction of nitrogen and density had a significant effect on the root length and surface area of unit area in the harvest stage in 2019. Compared with D1, the increase range of D3 in N1 treatment was significantly higher than that of N0 and N2. As to plant characteristics, nitrogen fertilizer and the interaction of nitrogen fertilizer and density were different between years and periods, and with the increase of density, the number of leaves of main stem, the number of lateral branches and the first internode thickness of main stem decreased. The effects of nitrogen fertilizer on pod yield was not significant, whereas pod yield increased with the increase of density. Pod yields were positively correlated with root volume, root dry weight, leaves of main stem, height of main stem and length of lateral branches. In conclusion, considering the yield and benefit comprehensively, the suitable nitrogen fertilizer (N) level is 60 kg hm-2 and the planting density is 188,000 plants hm-2.