Table 1

Primer sequences used for gene mapping"

Fig. 1

Phenotype of dek48 mutant in maize A: mature M4 ear; B: longitudinal section of wild type kernel; C: longitudinal section of dek48 kernel; D: F2 ear with B73 as the maternal parent; E: F2 ear with Mo17 as the maternal parent; F: F2 ear with C7-2 as the maternal parent.、"

Fig. 2

Hundred-kernel weight of wild type and mutant kernels ** Significant difference at P< 0.01."

Fig. 3

Determination and analysis of WT and dek48 kernel components A: starch content of WT and dek48; B: protein and oil content of WT and dek48. *, ** represent significant difference between the dek48 mutant and WT at the 0.05 and 0.01 probability levels, respectively."

Fig. 4

SEM observation of the endosperm of WT and dek48 A: SEM observation of WT, bar = 10 μm; B: SEM observation of dek48, bar = 10 μm; SG: starch granule; MP: matrix protein."

Fig. 5

Observation of embryo and endosperm of WT and dek48 at different development stages A: the observation and analysis of the embryo, endosperm and whole seed of dek48 at 12, 16, 20, 25, and 30 DAP, bar = 2 mm; B: the observation and analysis of the embryo, endosperm and whole seed of WT at 12, 16, 20, 25, and 30 DAP, bar = 2 mm."

Fig. 6

Paraffin section observation of WT and dek48 kernels at 16DAP A: embryos of WT seeds at 16 DAP, bar = 200 μm; B: embryo of dek48 seeds at 16 DAP, bar = 200 μm; C: aleurone layer of WT seeds at 16 DAP, bar = 50 μm; D: aleurone layer of dek48 seeds at 16 DAP, bar = 50 μm; AL: aleurone."

Table 2

Statistics and analysis of the segregation ratios of WT and dek48 mutants"

Fig. 7

Number of SNP markers"

Fig. 8

Distribution of SNP-index on whole genome"

Fig. 9

The fine mapping of dek48 mutant N: number of population; Recombinant: number of recombinants."

Table 3

Candidate genes annotation in the location interval"