紫甘薯SSR标记遗传图谱构建与重要农艺性状QTL定位

doi:10.3724/SP.J.1006.2021.04271

Abstract

Abstract:

Ideal agronomic traits are the important objectives in sweetpotato breeding, but the breeding methods are still lacking. We constructed linkage maps using a mapping population of 274 individuals derived from a cross between the female parent Xuzishu 8 (a purple-fleshed cultivar with many branches, medium vine, and high yield) and the male parent Meiguohong (a white-fleshed cultivar with few branches, long vine, and medium yield) by simple sequence repeats (SSR) markers in this study. The female parent map contained 24 linkage groups, and covered 1325.8 cM with an average marker interval of 9.2 cM. The male parent map contained 21 linkage groups, and covered 1088.6 cM with an average marker interval of 8.2 cM. The maps could increase the density of existing genetic maps. Using the composite interval mapping, we analyzed five important agronomic traits, including branch number, vine diameter, longest vine length, petiole length, and internode length in sweetpotato, thus identified one QTL related to branch number explaining the phenotypic variance of 53.2%, one QTL related to internode diameter explaining the phenotypic variance of 16.7%, two QTLs related to longest vine length explaining the phenotypic variance of 9.5% and 13.7%, two QTLs related to petiole length explaining the phenotypic variance of 8.8% and 11.3%, and five QTLs related to internode length explaining the phenotypic variance of 9.6%-28.1%. The QTLs can be used to develop molecular markers and assist the screening of plants with ideal agronomic traits at early seedling stage, thus improved the efficiency of field selection.

Key words: sweetpotato, the number of branches, vine diameter, the longest vine length, petiole length, internode length, QTLs

MA Meng, YAN Hui, GAO Run-Fei, KOU Meng, TANG Wei, WANG Xin, ZHANG Yun-Gang, LI Qiang. Construction linkage maps and identification of quantitative trait loci associated with important agronomic traits in purple-fleshed sweetpotato[J].Acta Agronomica Sinica, 2021, 47(11): 2147-2162.

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年份 Year	母本(个) Maternal	父本(个) Paternal	最大值(个) Max.	最小值(个) Min.	平均值(个) Mean	标准差 SD	偏度 Skewness	峰度 Kurtosis
2019	14.83	6.67	75.00	7.00	23.00	10.47	1.37	3.29
2020	14.20	8.00	25.80	2.33	11.95	3.70	0.64	1.03
平均Average	14.52	7.33	66.00	6.20	18.14	8.56	2.04	6.41

年份 Year	母本 Maternal (mm)	父本 Paternal (mm)	最大值 Max. (mm)	最小值 Min. (mm)	平均值 Mean (mm)	标准差 SD	偏度 Skewness	峰度 Kurtosis
2019	3.93	6.88	7.04	2.52	5.17	0.80	-0.21	0.39
2020	4.19	6.90	7.26	2.80	5.23	0.72	0.06	0.75
平均Average	4.06	6.89	7.19	2.96	5.19	0.72	-0.13	0.54

年份 Year	母本 Maternal (cm)	父本 Paternal (cm)	最大值 Max. (cm)	最小值 Min. (cm)	平均值 Mean (cm)	标准差 SD	偏度 Skewness	峰度 Kurtosis
2019	178.33	185.00	425.00	75.00	178.09	52.82	0.93	2.41
2020	182.00	195.00	361.00	77.00	172.74	47.83	0.50	0.60
平均Average	180.17	190.00	393.00	75.00	174.68	43.86	0.63	2.20

QTL名称 QTL name	连锁图谱 Linkage group	QTL位置 QTL location (cM)	年份 Year	遗传效应 Genetic effect	贡献率 R² (%)
qVLm-1p	Meiguohong (2)	5.006	2020	正向Positive	18.0
qVLm-1p	Meiguohong (2)	5.006	平均Average	正向Positive	13.7
qVLf-1p	Xuzishu 8 (18)	15.775	2019	正向Positive	12.4
qVLf-1p	Xuzishu 8 (18)	15.775	平均Average	正向Positive	9.5

年份 Year	母本 Maternal (cm)	父本 Paternal (cm)	最大值 Max. (cm)	最小值 Min. (cm)	平均值 Mean (cm)	标准差 SD	偏度 Skewness	峰度 Kurtosis
2019	14.17	15.48	27.98	5.80	16.74	4.18	0.47	-0.24
2020	17.94	10.92	26.64	8.80	18.60	3.16	-0.05	0.10
平均Average	16.05	13.20	25.24	5.80	17.54	3.38	0.02	-0.14

Construction linkage maps and identification of quantitative trait loci associated with important agronomic traits in purple-fleshed sweetpotato

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