棉籽油分和3种主要脂肪酸含量QTL分析

doi:10.3724/SP.J.1006.2022.04273

Abstract

Abstract:

In this study, in order to uncover more useful gene information for these traits, the analysis of QTLs controlling the contents of oil, palmitic acid, oleic acid, and linoleic acid in cottonseeds was conducted in different genetic systems. Based on a set of 188 recombinant inbred lines (RILs) derived from an intra-specific cross between two upland germplasms HS46 (P₁) and MARCABUCAG8US-1-88 (P₂), double backcross populations BC (P₁) and BC (P₂) were constructed in 2017 and 2018, respectively. BC (P₁) and BC (P₂) populations were obtained through backcrossing each of the two parents (HS46 and MARCABUCAG8US-1-88) with the 188 RILs, respectively. The cottonseeds from these backcross populations were used for QTLs analysis of oil, palmitic acid, oleic acid, and linoleic acid contents. To map QTLs for these traits, the mixed linear genetic model and the QTL Network-CL-2.0-Seed software, which were developed especially for seed traits, were applied. In total, seven QTLs for the oil content, three for the palmitic acid content, two for the oleic acid content, and three for the linoleic acid content were detected. Significant additive effects of QTLs from maternal plants and embryos were detected for all the QTLs. Seven QTLs, each of which explained more than 10% of total phenotypic variation, were found. The detected QTLs in the present study may give us better understanding of the molecular inheritance for these four traits, and provide more reliable information for molecular marker assisted breeding.

Key words: cotton, quantitative trait loci (QTL), oil content, fatty acid, embryo nuclear genome, maternal nuclear genome

ZHANG Yan-Bo, WANG Yuan, FENG Gan-Yu, DUAN Hui-Rong, LIU Hai-Ying. QTLs analysis of oil and three main fatty acid contents in cottonseeds[J].Acta Agronomica Sinica, 2022, 48(2): 380-395.

Figures/Tables 7

Table 1

Fig. 1

Table 2

Table 3

Genetic effects and detection of the QTLs for oil content and three fatty acid contents of cottonseed across environments"

性状 Trait	QTL	连锁群 Chr. / LG	标记区间 Marker interval	位置Position (cM)	范围 Range (cM)	a^e	d^e	a^m	a^eE1	a^eE2	d^eE1	d^eE2	a^mE1	a^mE2
油分含量 Oil content	qOil-3-1	Chr. 3	MUCS127(c3)-NAU808	4.8	2.0-7.3	9.49^**	0.05	-9.32^**	-0.02	-0.05	0.11	-0.10	0.02	0.06
	qOil-18-1	Chr. 18	JESPR204(c18)-CIR099(18)	0.0	0.0-1.0	-21.10^**	-0.10	20.88^**	0.09	0.00	-0.00	0.00	-0.09	-0.00
	qOil-18-2	Chr. 18	TM0409a-NAU1001	14.8	13.9-16.8	-5.02^**	-0.23^**	5.24^**	-0.07	-0.04	-0.07	0.07	0.07	0.03
	qOil-18-3	Chr. 18	NAU5152b-NAU3053a	76.7	72.3-79.7	-0.32^**	0.11^*	0.50^**	-0.12^*	-0.01	0.08	-0.08	0.12^*	0.01
	qOil-LG3-1	lg3	NAU5146c-RAPD-I10-620	51.4	48.6-58.4	13.45^**	-0.01	-13.72^**	0.07	0.08	0.00	-0.00	-0.07	-0.08
	qOil-LG7-1	lg7	NAU983-TMB1791	0.0	0.0-2.0	0.81^**	-0.28^**	-0.90^**	-0.00	0.22^**	-0.22^*	0.21^**	-0.00	-0.22^**
	qOil-LG8-1	lg8	NAU4919b^*-NAU4960d	31.7	30.7-34.5	1.84^**	0.05	-1.48^**	-0.16^*	0.00	0.07	-0.09	0.16^*	-0.01
棕榈酸含量 Palmitic acid content	qC_16-0-15-1	Chr. 15	BNL1579-BNL4082(c15)	25.1	23.1-27.0	5.72^**	-0.06^*	-5.66^**	-0.02	-0.00	0.00	-0.00	0.02	0.00
	qC_16-0-LG3-1	lg3	NAU5146c-RAPD-I10-620	56.4	50.6-62.4	-3.88^**	0.04	3.74^**	0.11^**	-0.13^**	-0.00	0.00	-0.11^**	0.13^**
	qC_16-0-LG6-1	lg6	NAU4852-NAU1247	40.5	39.2-42.5	-2.02^**	-0.04^*	2.10^**	-0.01	-0.00	0.05	-0.05	0.02	0.00
油酸含量 Oleic acid content	qC_18-1-18-1	Chr. 18	NAU5152a-NAU1232	44.6	40.6-47.6	-1.44^**	0.01	1.28^**	0.21^**	-0.20^**	0.00	-0.00	-0.21^**	0.20^**
油酸含量 Oleic acid content	qC_18-1-LG6-1	lg6	NAU456-NAU5328-170	19.9	17.9-21.9	1.74^**	-0.09^**	-1.83^**	0.10^**	-0.02	-0.10^*	0.09^*	-0.10^**	0.02
亚油酸含量 Linoleic acid content	qC_18-2-6-1	Chr. 6	NAU932^*-NAU2566	81.4	75.4-91.0	10.47^**	0.01	-10.60^**	0.04	0.00	0.11^*	-0.11^*	-0.04	-0.00
	qC_18-2-15-1	Chr. 15	BNL1579-BNL4082(c15)	25.1	23.1-27.0	-4.83^**	0.08^*	4.71^**	-0.27^**	0.29^**	-0.00	0.00	0.26^**	-0.28^**
	qC_18-2-LG6-1	lg6	NAU5328-170-RAPD-G17-120	23.0	22.0-24.0	-5.45^**	-0.09^*	5.64^**	-0.00	-0.05	0.15^**	-0.15^**	0.00	0.04

Table 3

Fig. 2

Table 4

Table S1

Information from molecular genetic marker related to QTL for four traits of cottonseeds"

染色体/连锁群 Chr./Linkage group	分子标记 Molecular markers	引物 Primers (5°-3°)	标记类型 Marker type	标记引物来源 Source of marker primer	标记来源 Source of marker	重复基序 Repeat motif
Chr3	MUCS127(c3)	F: TTTGTACTCATGTTCCCGCC; R: GCATGCTTGAAGAAGGTTCC	SSR	Park et al.^[40]	G. arboreum EST	TCTT(2)CTT(4)
Chr3	NAU808	F: GATGGCTACCTCCCTTTGTA; R: CGTAAGGAAGCCTAGCAAAA	SSR	Han et al. ^[41,42]	G. hirsutum EST	none
Chr18	JESPR204(c18)	F: CTCCAGGTTCAATGGTCTG; R: GCCATGTTGGACAAGTAGTC	SSR	Reddy et al.^[43]	G. hirsutum genome	(CTT)20
Chr18	CIR099(18)	F: ATGATTCAAGTCGCGT; R: TTCAAGGCTGAGTCAAA	SSR	Nguyen et al.^[44]	G. hirsutum genome	(GT)8
Chr18	TMO409a	F: CAGAGGACGAAGGTAGCAG; R: TGGTGGGTTTCACTTTCACA	SSR	USDA-ARS	G. hirsutum genome	(CA)10+(GA)5
Chr18	NAU1001	F: TAAAGGAAGAAAACCCACCA; R: TTTCTCTTCAACAGCAGCAG	SSR	Han et al.^[41,42]	G. hirsutum EST	(ACA )7
Chr18	NAU5152b	F: TCCTTTCTACCCATGCCTAC; R: GTCACGAGAAGCAGAGGACT	SSR	Han et al.^[41,42]	G. hirsutum EST	(tct)9
Chr18	NAU3053a	F: TTGGAGGTGTCAGATCCTCT; R: AAGATCCATGACCCAGAAAA	SSR	Han et al.^[41,42]	G. hirsutum EST	(at)13
LG3	NAU5146c	F: ATCTCCATTCCCTCTCCTCT; R: ATAAACGCAACGGATAAAGG	SSR	Han et al.^[41,42]	G. hirsutum EST	(ttta)5
LG3	RAPD-I10-620	GTCCACACGG	RAPD	购于上海赛百盛生物技术公司 (The marker primer purchased from SBS Genetech Co.,Ltd)	G. hirsutum genome	None
LG7	NAU983	F: GAAGGTCATGTTTTGCATTG; R: AGCCATCAAATGACCAGTCT	SSR	Han et al.^[41,42]	G. hirsutum EST	(CAGCAT)3
LG7	TMB1791	F: GAGATCGTTTATCATTCCCACA; R: TCTACCGGCCTCGTAAGCTA	SSR	Yu et al.^[45]	G. hirsutum genome	(GA)15+(CA)8
LG8	NAU4919b*	F: ATCTAATGGGGGAGGAAGAG; R: CTCCTTGAAACCCTTTGAGA	SSR	Han et al.^[41,42]	G. hirsutum EST	(cca)6
LG8	NAU4960d	F: CGGCCTACCTCTTCCTCT; R: TTAACGACAATTCCATCGTG	SSR	Han et al.^[41,42]	G. hirsutum EST	(tcc)6
Chr15	BNL4082(c15)	F:GTAAAATGAAATAAAATAAAAGGAGAGA; R: TTCAACACCGCCAAACATAA	SSR	Brookhaven National Laboratory, NY, USA	G. hirsutum genome	(GA)24
LG3	NAU4852	F: ATCTCAAGGCCCAAATTACA; R: CCTAAATTGTCAAGCGAGGT	SSR	Han et al.^[41,42]	G. hirsutum EST	(cat)6
LG3	NAU1247	F: AATTGGCTTCCAAGAGACTG; R: CACCATCATCATCAAACTCG	SSR	Han et al.^[41,42]	G. hirsutum EST	(AAG)8
Chr18	NAU5152a	F: TCCTTTCTACCCATGCCTAC; R: GTCACGAGAAGCAGAGGACT	SSR	Han et al.^[41,42]	G. hirsutum EST	(tct)9
Chr18	NAU1232	F：GTGGAATGTGGTGATGAGAA; R：CAATCCCACCCCATATCTA	SSR	Han et al.^[41,42]	G. hirsutum EST	(TGTA)5
LG6	NAU456	F: GAAGGAGGGCGAAAAACATA; R: TTGGAGATTACGCGACCTTC	SSR	Han et al.^[41,42]	G. hirsutum EST	(CAC )8
LG6	NAU5328-170	F: AATGAGAGTGGCGGCTATAC; R: CTTATAGCTCTGGGCAGCAT	SSR	Han et al.^[41,42]	G. hirsutum EST	(aatgca)3
Chr6	NAU932*	F: ACAAAGCAAATATGGGTTCC; R: CCCCCTTAAAATGGAAGAAG	SSR	Han et al.^[41,42]	G. hirsutum EST	(GGTGAA)3
Chr6	NAU2566	F: CAAAATCCACCCCCTCCT; R: CCCTTCCCAGGAAAGTTTAT	SSR	Han et al.^[41,42]	G. hirsutum EST	(aata)4
Chr15	BNL1579	F: CATGAGAACTTCATGCATATTCA; R: ATTTAAGTGCCTCCGTCCCT	SSR	Brookhaven National Laboratory, NY, USA	G. hirsutum genome	(AG)17
LG6	RAPD-G17-120	GACGGATCAG	RAPD	购于上海赛百盛生物技术公司 (The marker primer purchased from SBS Genetech Co., Ltd)	G. hirsutum genome	None

Table S1

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年份Year	性状 Trait	回交群体BC(P₁)						回交群体BC(P₂)						亲本Parents
年份Year	性状 Trait	平均值 Mean	标准差 SD	最小值 Min.	最大值 Max.	偏度 Skew	峰度 Kurt	平均值 Mean	标准差 SD	最小值 Min.	最大值 Max.	偏度 Skew	峰度 Kurt	HS46	MAR
2017	油分含量 Oil content	32.28	1.85	25.26	38.06	-0.23	0.12	33.05	1.98	27.59	37.30	-0.18	-0.17	31.01 a	33.49 b
	棕榈酸含量 Palmitic acid content	25.66	0.63	24.19	27.40	0.13	-0.07	25.73	0.67	23.82	27.75	-0.38	0.56	25.49 a	25.94 b
	棕榈酸含量 Palmitic acid content	25.66	0.63	24.19	27.40	油酸含量 Oleic acid content	17.86	25.73	0.67	23.82	27.75	0.80	15.84	25.49 a	25.94 b	20.53	0.34	0.34	17.51	1.01	14.88	20.34	-0.11	0.54	17.31	17.01
	亚油酸含量 Linoleic acid content	56.90	1.16	53.81	59.79	油酸含量 Oleic acid content	17.86	-0.13	-0.31	56.85	1.29	0.80	15.84	52.80	60.85	20.53	-0.14	0.88	17.51	1.01	14.88	20.34	57.55 a	56.81 b	17.31	17.01
	亚油酸含量 Linoleic acid content	56.90	1.16	53.81	59.79	2018	油分含量 Oil content	32.86	0.92	56.85	1.29	29.58	35.08	52.80	60.85	-0.80	0.75	33.58	0.97	30.94	36.82	-0.22	57.55 a	56.81 b	0.29	29.70 A	32.69 B
	棕榈酸含量 Palmitic acid content	24.72	0.57	23.13	26.45		0.17	0.36	25.16	0.51	23.26	27.17	-0.37	0.09	24.63 A	25.22 B
	棕榈酸含量 Palmitic acid content	24.72	0.57	23.13	26.45		油酸含量 Oleic acid content	17.38	25.16	0.51	23.26	27.17	0.68	15.63	24.63 A	25.22 B	19.15	0.10	-0.07	17.02	0.59	15.24	18.85	-0.01	0.33	16.60 a	16.21 b
亚油酸含量 Linoleic acid content	58.47	0.97	55.76	61.14	-0.05		油酸含量 Oleic acid content	17.38	0.21	58.06	0.70	56.16	0.68	15.63	59.80	-0.03	19.15	0.12	59.06	17.02	0.59	15.24	18.85	58.45		16.60 a	16.21 b

性状 Trait	油分 Oil content	棕榈酸 Palmitic acid content	油酸 Oleic acid content	亚油酸 Linoleic acid content
油分Oil content	1.00000
棕榈酸Palmitic acid content	0.45145^**	1.00000
油酸Oleic acid content	-0.14629^**	0.15922^**	1.00000
亚油酸Linoleic acid content	-0.13874^**	-0.64037^**	-0.73753^**	1.00000

QTLs analysis of oil and three main fatty acid contents in cottonseeds

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