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Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (9): 2221-2227.doi: 10.3724/SP.J.1006.2022.11085

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• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Breeding of FHB-resistant wheat line Bainong 4299 by gene pyramiding

ZHANG Yi-Duo1(), LI Guo-Qiang1, KONG Zhong-Xin1, WANG Yu-Quan2, LI Xiao-Li2, RU Zhen-Gang2, JIA Hai-Yan1,*(), MA Zheng-Qiang1   

  1. 1. Crop Genomics and Bioinformatics Center, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
    2. Center of Wheat Research, Henan Institute of Science and Technology, Xinxiang 450003, Henan, China
  • Received:2021-09-29 Accepted:2021-11-29 Online:2022-09-12 Published:2022-12-27
  • Contact: JIA Hai-Yan E-mail:2014201043@njau.edu.cn;hyjia@njau.edu.cn
  • Supported by:
    National Key Research and Development Program of China(2016YFD0101802);National Natural Science Foundation of China(31930081);National Natural Science Foundation of China(30430440);Collaborative Innovation Initiative for Modern Crop Production

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Abstract:

Fusarium head blight (FHB) is a devastating fungal disease in wheat production. Wheat FHB resistance is controlled by multiple genes and has complicated resistance mechanisms. Type I (resistance to invasion) and type II (resistance to expansion) are two main resistance types of wheat against FHB. Combining both types of FHB resistance in breeding is vitally important for the resistance durability and stability of cultivars. In fine mapping and cloning of type I resistance QTL Fhb4 and Fhb5 and type II resistance QTL Fhb1 in wheat landrace Wangshuibai, functional/tightly-linked molecular markers for them had been obtained. In this study, a new wheat line named Bainong 4299 was bred after introduction of these QTL from NMAS022 with the help of these markers and using modern wheat variety Bainong 4199 as the recipient parent. Compared with Bainong 4199, Bainong 4299 increased type I resistance by at least 73% to 74% and type II resistance by 83% to 88% increase (in terms of the number of diseased spikelets per spike) in two field trials. Moreover, its yield potential had moderate elevation. In conclusion, this study provided another successful illustration of marker-assisted selection and pyramiding of FHB QTL in improving wheat FHB resistance. Bainong 4299 had the potential to become a new FHB resistance cultivar.

Key words: wheat, Fusarium head blight, pyramiding of resistance genes, MAS, Bainong 4299

Fig. 1

Detection of Fhb1, Fhb4, and Fhb5 in Bainong 4199, NMAS022, and Bainong 4299 M: DNA marker. Band sizes are on the left of each picture (unit: bp); 1: Bainong 4199; 2: NMAS022; 3: Bainong 4299. The target bands of Wangshuibai type are marked with arrows respectively, and primer names are at the bottom of each picture."

Fig 2

Breeding process of Bainong 4299 (a) and its donor parent NMAS022 (b) n: population size; The picture (b) was taken on May 4, 2020 in Huai’an, Jiangsu province, China."

Table 1

FHB resistance identification of Bainong 4299 and its recurrent parent"

环境
Environment		 材料
Material		 赤霉病严重度
FHB severity		 病穗率
Percentage of infected spikes (%)		 病小穗数
Number of diseased spikelets		 病轴长
Length of diseased rachides (cm)
2019-2020淮安
2019-2020 Huai’an		 百农4199 Bainong 4199 2.23±0.11 38.5±1.8 5.8±0.6 4.1±0.5
百农4299 Bainong 4299 0.10±0.02** 10.2±2.2** 1.0±0.0** 0.8±0.1**
2020-2021南京
2020-2021 Nanjing		 百农4199 Bainong 4199 3.44±0.14 42.3±2.1 8.2±0.1 4.1±0.1
百农4299 Bainong 4299 0.11±0.01** 11.5±1.3** 1.0±0.0** 0.6±0.0**

Fig. 3

Fusarium head blight symptom illustration of Bainong 4299 (left) and its recurrent parent Bainong 4199 (right) in the field under natural condition"

Table 2

Agronomic traits and yield identification of Bainong 4299 and its recurrent parent"

环境
Environment		 材料
Material		 株高
Plant height (cm)		 穗粒数
No. of kernels per spike		 单株穗数
No. of spikes per plant		 千粒重
1000-kernel weight (g)		 1 m2产量
Yield per square meter (g)
2019-2020淮安
2019-2020 Huai’an		 百农4199 Bainong 4199 80.2±1.2 54.4±1.8 12.1±0.6 44.2±0.4 984.5±3.5
百农4299 Bainong 4299 84.2±0.9** 55.0±1.9 12.0±0.9 48.4±0.5** 1002.0±4.2*
2020-2021淮安
2020-2021 Huai’an		 百农4199 Bainong 4199 82.0±1.2 42.3±1.6 4.4±0.8 46.3±0.4 845.9±5.8
百农4299 Bainong 4299 86.9±1.7** 39.0±1.6** 5.3±1.2 44.7±0.2** 874.8±3.4*
2020-2021南京
2020-2021 Nanjing		 百农4199 Bainong 4199 72.6±1.8 42.5±0.8 3.8±0.1 41.5±0.2 490±5.2
百农4299 Bainong 4299 72.0±1.2 37.1±1.1** 4.6±0.3 42.8±0.2** 520±4.7*
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