利用中国抗源区分强致病力大豆胞囊线虫群体的探讨

doi:10.3724/SP.J.1006.2022.14188

Abstract

Abstract:

The disease caused by soybean cyst nematode (soybean cyst nematode, Heterodera glycines, SCN) is a worldwide soybean disease. The internationally used race identification models including Riggs model and Hg type for differentiate soybean cyst nematode population could not effectively distinguish the highly virulent race such as race 4, X12 population, and synthesized nematode population LY1. This study provides a simple identification method for distinguishing the three highly virulent population of the soybean cyst nematode, which would be greatly contribute to SCN research. This method included the following steps: First, we used the susceptible varieties and raised enough cysts in the soil infected highly virulent population of SCN for inoculation. Then, soybean varieties Xingxianhuipizhi (ZDD2315) and PI567516C were selected as differential hosts, and Lee was used as susceptible control for inoculation. Both ZDD2315 and PI567516C was sensitive in the inoculating reaction, indicating that the soil was infected by X12 population. If ZDD2315 was resistance while PI567516C were susceptible to the disease, indicating the soil was infected by race 4. If PI567516C was resistance to disease, it revealed that the soil was infected by LY1 population. These results indicated that the three highly virulent population including race 4, X12 population, and synthesized LY1 population could be effectively distinguished depending on the reaction of SCN on germplasms of ZDD2315 and PI567516C from China. In conclusion, these results of this study are of great significance for screening resistance germplasms, investigating the race distribution of SCN, and studying the pathogenic genes of SCN.

Key words: soybean cyst nematode, race, highly virulent, germplasms

LIAN Yun, WEI He, WANG Jin-She, ZHANG Hui, LEI Chen-Fang, LI Jin-Ying, LU Wei-Guo. Identification highly virulent population of soybean cyst nematode using China germplasms[J].Acta Agronomica Sinica, 2022, 48(10): 2443-2450.

Figures/Tables 4

Table 1

Table 2

Table 3

Table 4

Verify race 4, race 2, and X12 population using multiple soil samples with ZDD2315 and PI567516C"

生理小种 Race	生理小种鉴定Race testing	抗性反应Reaction		土样编号 Soil code	土样来源 Origin of the soil		土样采集时间 Soil sample collecting time (month/day)	土样采集地点 Soil sample collecting location
生理小种 Race	Lee^*	PI567516C	ZDD2315	土样编号 Soil code	经度 Longitude (°)	纬度 Latitude (°)	土样采集时间 Soil sample collecting time (month/day)	土样采集地点 Soil sample collecting location
X12	185.4±26.9 (144-214)	+	+	SX12	112.166	37.844	2012/7	山西省邢家社乡想儿岭村 Xiangerling village, Xingjiashe township, Shanxi province
X12	118.4±28.7 (62-158)	+	+	GJ30	112.257	38.014	2019/8	山西省河口镇段家岭村 Duanjialing village, Hekou town, Shanxi province
X12	135±38.7 (85-176)	+	+	GJ07	112.166	37.844	2019/8	山西省邢家社乡想儿岭村 Xiangerling village, Xingjiashe township, Shanxi province
X12	222.9±36.2 (159-265)	+	+	SX12单胞囊繁殖 SX12 Raised from single cyst	112.166	37.844	2016/1	河南省农业科学院培育 Cultivated from Henan Academy of Agricultural Sciences
Race 4	122.4±23.2 (97-154)	+	-	GJ05	112.190	37.758	2019/8	山西省邢家社乡郭家社村 Guojiashe village, Xingjiashe township, Shanxi province
Race 4	156.3±33.1 (118-192)	+	-	GJ21	111.967	37.846	2019/8	山西省马兰镇南龙沟村 Nanlonggou village, Malan town, Shanxi province
Race 4	189±42.5 (129-229)	+	-	GJ27	112.218	37.998	2019/8	山西省河口镇大坡村 Dapo village, Hekou town, Shanxi province
Race 4	110.4±21 (78-143)	+	-	GJ14	112.070	37.876	2019/8	山西省马兰镇姬家庄村 Jijiazhuang village, Malan town, Shanxi province
Race 4	134±62.2 (45-260)	+	-	GJ28	112.232	38.017	2019/8	山西省河口镇水头村 Shuitou village, Hekou town, Shanxi province
Race 4	124.6±26.1 (87-165)	+	-	SX37	113.232	38.477	2012/7	山西省忻州市定襄县南庄村 Nanzhuang village, Dingxiang county, Xinzhou city, Shanxi province
Race 4	168±26.6 (135-212)	+	-	SX49	112.802	39.484	2012/7	山西省朔州市山阴县安荣乡 An Rongxiang, Shanyin county, Shuozhou city, Shanxi province
Race 4	220±42.8 (138-270)	+	-	SX51	112.468	39.511	2012/7	山西省朔州市平鲁区李西沟村 Lixigou village, Pinglu district, Shuozhou city, Shanxi province
Race 4	187.1±33.9 (130-222)	+	-	SX59	112.613	36.935	2012/7	山西省长治市沁县西汤村 Xitang village, Qin county, Changzhi city, Shanxi province
Race 4	137.1±36 (90-196)	+	-	SX37	113.232	38.477	2012/7	山西省忻州市定襄县南庄村 Nanzhuang village, Dingxiang county, Xinzhou city, Shanxi province
Race 2	170.4±50.6 (106-252)	+	-	GJ 08	111.960	37.761	2019/8	山西省常安乡小娄峰村 Xiao Loufeng village, Chang'an township, Shanxi province
Race 2	95±32 (43-127)	+	-	GJ 02	112.161	37.860	2019/8	山西省桃园办事处李家社村 Lijiashe village, Taoyuan office, Shanxi province
Race 2	659±283.5 (297-1138)	+	-	GJ 04	112.188	37.785	2019/8	山西省邢家社乡龙子村 Longzi village, Xingjiashe township, Shanxi province
Race 2	286±80.5 (164-397)	+	-	GJ 12	112.05	37.816	2019/8	山西省常安乡麻家口村 Majiakou village, Chang'an township, Shanxi province
Race 2	242.3±113 (74-454)	+	-	GJ 16	111.924	37.861	2019/8	山西省岔口乡板四沟村 Bansigou village, Chakou township, Shanxi province
Race 2	112.3±44.7 (41-166)	+	-	GJ 21	111.967	37.846	2019/8	山西省马兰镇南龙沟村 Nanlonggou village, Malan town, Shanxi province

Table 4

References 27

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寄主 Differential host	接种X12线虫群体 Inoculating X12 population			接种4号生理小种 Inoculating race 4			接种LY1线虫群体^* Inoculating LY1 population^*
寄主 Differential host	每株上的平均胞囊数目^ Average cysts number per plant^	胞囊指数 FI	抗性反应 Reaction	每株上的平均胞囊数目^ Average cysts number per plant^	胞囊指数 FI	抗性反应 Reaction	每株上的平均胞囊数目 Average cysts number per plant	胞囊指数 FI	抗性反应 Reaction
Lee	185.4±26.9 (144-214)			330.6±62.8 (237-401)			100
Pickett	181.6±87.7 (52-264)	98	+	310.6±64.0 (251-413)	94	+	/
Peking	95.2±91.5 (25-206)	51.3	+	292.0±21.3 (270-318)	88.3	+	/	83	+
PI88788	177.6±94.1 (103-339)	95.8	+	290.0±93.8 (143-384)	87.7	+	/	45	+
PI90763	93.0±47.9 (26-133)	50.2	+	99.6±21.0 (78-127)	30.1	+	/	92	+

寄主 Differential host	接种X12线虫群体Inoculating X12 population			接种4号生理小种 Inoculating race 4			接种LY1线虫群体^* Inoculating LY1 population^*
寄主 Differential host	每株上的平均胞囊数目^ Average cysts number per plant^	胞囊指数 FI	抗性反应 Reaction	每株上的平均胞囊数目^ Average number of cysts per plant^	胞囊指数 FI	抗性反应 Reaction	每株上的平均胞囊数目 Average cysts number per plant	胞囊指数 FI	抗性反应 Reaction
Lee	185.4±26.9 (144-214)	/		330.6±62.8 (237-401)	/		100
Peking	95.2±91.5 (25-206)	51.3	+	292.0±21.3 (270-318)	88.3	+	/	83	+
PI88788	177.6±94.1 (103-339)	95.8	+	290.0±93.8 (143-384)	87.7	+	/	45	+
PI90763	93.0±47.9 (26-133)	50.2	+	99.6±21.0 (78-127)	30.1	+	/	92	+
PI437654	51.2±23.1 (11-66)	27.6	+	23.6±6.4 (17-34)	7.1	-	/	260	+
PI89772	179.5±51.5 (122-239)	96.8	+	54.0±5.7 (50-58)	16.3	+	/	172	+
PI209332	235.0±46.7 (181-282)	126.8	+	421.2±86.8 (344-532)	127.4	+	/	55	+
PI548316	224.8±49.2 (156-270)	121.2	+	251.8±98.6 (141-394)	76.2	+	/	130	+

寄主 Differential	接种X12线虫群体Inoculating X12 population			接种4号生理小种 Inoculating race 4			接种LY1线虫群体^* Inoculating LY1 population^*
寄主 Differential	每株上的平均胞囊数目^ Average cysts number per plant^	胞囊指数 FI	抗性反应 Reaction	每株上的平均胞囊数目^ Average cysts number per plant^	胞囊指数 FI	抗性反应 Reaction	每株上的平均胞囊数目^ Average cysts number per plant^	胞囊指数 FI	抗性反应 Reaction
Lee	185.4±26.9 (144-214)	/		330.6±62.8 (237-401)	/		100
ZDD2315	145.4±87.7 (52-264)	87.7	+	14.0±2.8 (12-16)	4.2	-		/
PI567516C	225±51.5 (122-239)	121.4	+	80.2±32.8 (53-135)	24.2	+		7	-

Identification highly virulent population of soybean cyst nematode using China germplasms

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