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作物学报 ›› 2009, Vol. 35 ›› Issue (1): 11-17.doi: 10.3724/SP.J.1006.2009.00011

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

条锈菌诱导的小麦MBF1转录辅激活因子基因的克隆及其特征分析

张毅1;张岗1;董艳玲1;郭军1;黄丽丽1;康振生12*   

  1. 1西北农林科技大学植物保护学院,陕西杨凌 712100;2 西北农林科技大学陕西省农业分子生物学重点实验室,陕西杨凌 712100
  • 收稿日期:2008-06-23 修回日期:2008-07-09 出版日期:2009-01-12 网络出版日期:2008-11-17
  • 通讯作者: 康振生
  • 基金资助:

    本研究由国家重点基础研究发展计划(973计划)项目(2006CB708208),国家高新技术研究发展计划(863计划)重大研究项目(2006AA10A104),教育部科学技术研究重点项目(107104),教育部长江学者和创新团队发展计划项目(IRT0558),国家自然科学基金项目(30671350),高等学校学科创新引智计划项目(B07049)资助。

Cloning and Characterization of a MBF1 Transcriptional Coactivator Factor in Wheat Induced by Stripe Rust Pathogen

  • Received:2008-06-23 Revised:2008-07-09 Published:2009-01-12 Published online:2008-11-17
  • Contact: KANG Zhen-Sheng

摘要:

应用电子克隆和RT-PCR方法,从小麦叶片中分离出一个条锈菌诱导的编码MBF1基因的cDNA序列,暂被命名为TaMBF1aTaMBF1a包含一个完整的429 bp的开放阅读框,编码142个氨基酸,具有MBF1保守结构域;小麦TaMBF1a氨基酸序列与水稻OsMBF1相似性达92%,与拟南芥AtMBF1a相似性达80%TaMBF1a编码的蛋白可能是核蛋白,且该基因在小麦根、茎、叶组织中表达量基本一致。在小麦与条锈菌的亲和、非亲和互作中,TaMBF1a基因均受条锈菌诱导高水平表达,且非亲和组合表达量高于亲和组合。外源植物激素水杨酸、乙烯、脱落酸也可诱导该基因快速上调表达,表明TaMBF1a可能通过水杨酸、乙烯等信号途径参与小麦对条锈菌的防御反应。

关键词: 条锈菌, 小麦, MBF1转录辅激活因子, 电子克隆, 基因表达

Abstract:

To better understand wheat (Triticum aestivum L.) defense responses to Puccinia striiformis f. sp. tritici, the compatible interaction cDNA library of wheat leaves infected by Puccinia striiformis f. sp. tritici is constructed in our laboratory. A total of 594 genes have been identified and 399 genes have been annotated. On the basis of previous study, a new MBF1 gene was isolated from this cDNA library through in silico cloning and RT-PCR approaches. The gene was tentatively designated as TaMBF1a, whose open reading frame was 429 bp in length and encoded 142 amino acids containing a conserved MBF1 transcription activation domain. The amino acid sequence of TaMBF1a shares 92% identify with OsMBF1 in rice and 80% identify with AtMBF1a in Arabidopsis thaliana. The expression of TaMBF1a gene was at a similar level in leaves, stems, and roots. TaMBF1a protein is possibly a nuclear protein in wheat. The expression patterns results revealed that TaMBF1a was up-regulated in both compatible and incompatible interactions. However, the expression in incompatible interaction was higher than that in compatible interaction. The expression of TaMBF1a was also induced by salicylic acid (SA), ethylene, and abscisic acid (ABA), suggesting that the SA and ethylene pathways might be involved in regulating the host defence responses.

Key words: Stripe rust fungus, Wheat, Multiprotein bridging factor 1(MBF1), In silico cloning, Gene expression

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