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作物学报 ›› 2020, Vol. 46 ›› Issue (7): 1006-1015.doi: 10.3724/SP.J.1006.2020.93048

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

兼容型maizeSNP384标记筛选与玉米杂交种DNA指纹图谱构建

田红丽**,杨扬**,王璐,王蕊,易红梅,许理文,张云龙,葛建镕,王凤格(),赵久然()   

  1. 北京市农林科学院玉米研究中心/玉米DNA指纹及分子育种北京市重点实验室, 北京100097
  • 收稿日期:2019-08-30 接受日期:2020-03-24 出版日期:2020-07-12 发布日期:2020-04-17
  • 通讯作者: 王凤格,赵久然 E-mail:gege0106@163.com;maizezhao@126.com
  • 作者简介:田红丽, E-mail: tianhongli9963@163.com|杨扬, E-mail: caurwx@163.com
    ** 同等贡献
  • 基金资助:
    “十三五”国家重点研发计划项目(2017YFD0102001-003);北京学者计划项目(BSP041)

Screening of compatible maizeSNP384 markers and the construction of DNA fingerprints of maize varieties

TIAN Hong-Li**,YANG Yang**,WANG Lu,WANG Rui,YI Hong-Mei,XU Li-Wen,ZHANG Yun-Long,GE Jian-Rong,WANG Feng-Ge(),ZHAO Jiu-Ran()   

  1. Maize Research Center, Beijing Academy of Agriculture and Forestry Sciences / Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing 100097, China
  • Received:2019-08-30 Accepted:2020-03-24 Online:2020-07-12 Published:2020-04-17
  • Contact: Feng-Ge WANG,Jiu-Ran ZHAO E-mail:gege0106@163.com;maizezhao@126.com
  • About author:** Contributed equally to this work
  • Supported by:
    13th Five-Year National Key Research and Development Program of China(2017YFD0102001-003);Beijing Scholars Program(BSP041)

摘要:

为加强玉米品种管理和知识产权保护, 评估确定了一套兼容多技术平台, 适于玉米分子鉴定的SNP位点组合, 该组合包含384个位点; 构建了335个玉米杂交种SNP-DNA指纹, 并针对位点和样品从各个角度进行分析。384个位点全部分布在基因内区域, 显示了较好的多态性; MAF、PIC、DP平均值分别为0.39、0.36、0.60, 384个位点中88%的位点MAF值高于0.30, 98%的位点PIC值高于0.30, 98%的位点DP值高于0.50。对335个玉米杂交种进行遗传相似系数两两比较, GD (1 - Nei遗传距离)的变异范围为0.60~0.99, GD≥0.98、0.95、0.90者分别占比0.10%、0.38%、1.40%; GS (相同等位基因比)的变异范围为0.50~0.99, GS≥0.98、0.95、0.90者分别占比0.03%、0.11%、0.35%。从384个位点中抽取最优位点组合, 12个位点组合时品种识别率为0.99, 20个位点组合能够识别335个品种。综上所述, 本研究报道的384个核心SNP位点具有兼容多平台、高稳定性、高重复性、高品种区分能力, 利用核心位点构建了335个玉米杂交种SNP-DNA指纹, 为玉米品种分子鉴定、指纹数据构建以及分子育种提供了关键数据支撑。

关键词: 玉米, SNP, 位点组合, 品种分子鉴定, DNA指纹图谱, 芯片

Abstract:

In order to improve the management of maize varieties and the protection of intellectual property rights, a compatible multiple platform loci combination maizeSNP384 was developed as a means of identifying varieties of maize. SNP-DNA fingerprints of 335 maize hybrids were constructed. All the 384 SNPs were located in the intra-genic region, showing good polymorphism. The average MAF, PIC, and DP of 384 loci were 0.39, 0.36, and 0.60, respectively. MAF in 88% of the 384 loci was most than 0.30, PIC in 98% was most than 0.30, and DP in 98% was most than 0.50. Pairwise comparative analysis of 335 maize hybrids using genetic similarity showed that the range of variation of GD (1-Nei genetic distance) was 0.60-0.99, and the ratios of GD value greater than or equal to 0.98, 0.95, and 0.90 were 0.10%, 0.38%, and 1.40%, respectively. The range of variation of GS (same allele ratio) was 0.50-0.99, and the ratios of GS value greater than or equal to 0.98, 0.95, and 0.90 were 0.03%, 0.11%, and 0.35%, respectively. The optimal locus combination was extracted from 384 SNPs, with the variety discrimination power of 0.99 when 12 SNPs were extracted; the combination of 20 SNPs was able to identify all 335 varieties. In summary, 384 core SNPs reported in this study are compatible with multiple platforms, highly stable, highly repeatable and having high variety discrimination power. SNP-DNA fingerprints of 335 maize hybrids were constructed based on these core SNPs, which provides key data support for molecular identification, fingerprint data construction, and molecular breeding of maize.

Key words: maize, SNP, loci combination, variety molecular identification, DNA fingerprint, chip

表1

用于评估SNP位点的30套杂交种及其双亲的样品信息"

编号
No.
品种
Variety
母本
Female parent
父本
Male parent
T01 SC704 B73 Mo17
T02 丹玉39 Danyu 39 C8605-2 丹598 Dan 598
T03 登海11 Denghai 11 DH65232 DH40
T04 登海9号 Denghai 9 DH65232 8723
T05 东单60 Dongdan 60 A801 丹598 Dan 598
T06 会单4号 Huidan 4 SW1611 掖107 Ye 107
T07 吉单180 Jidan 180 吉853 Ji 853 Mo17
T08 吉单209 Jidan 209 8902 吉853 Ji 853
T09 京科968 Jingke 968 京724 Jing 724 京92 Jing 92
T10 京科糯2000 Jingkenuo 2000 京糯6 Jingnuo 6 白糯6 Bainuo 6
T11 浚单20 Xundan 20 浚9058 Xun 9058 浚92-8 Xun 92-8
T12 蠡玉6号 Liyu 6 543 连87 Lian 87
T13 龙单13 Longdan 13 K10 龙抗11 Longkang 11
T14 鲁单981 Ludan 981 齐319 Qi 319 Lx 9801
T15 农大108 Nongda 108 X178 黄C Huang C
T16 农大3138 Nongda 3138 综31 Zong 31 P138
T17 农华101 Nonghua 101 NH60 S121
T18 沈单10 Shendan 10 沈137 Shen 137 Q1261
T19 沈单16 Shendan 16 K12 沈137 Shen 137
T20 四单19 Sidan 19 444 Mo17
T21 苏玉糯1号 Suyunuo 1 通系5号 Tongxi 5 衡白522 Hengbai 522
T22 苏玉糯5号 Suyunuo 5 通系5号 Tongxi 5 通366 Tong 366
T23 先玉335 Xianyu 335 PH6WC PH4CV
T24 掖单13 Yedan 13 掖478 Ye 478 丹340 Dan 340
T25 渝单8号 Yudan 8 掖478 Ye 478 渝561 Yu 561
T26 渝糯7号 Yunuo 7 S147 S181
T27 豫玉22 Yuyu 22 综3 Zong 3 豫自87-1 Yuzi 87-1
T28 正大619 Zhengda 619 F06 F19
T29 郑单958 Zhengdan 958 郑58 Zheng 58 昌7-2 Chang 7-2
T30 中单2号 Zhongdan 2 Mo17 自330 Zi 330

图1

SNP位点(MG004)在3种芯片平台上的分型图 A: illumina GGGT芯片平台; B: illumina infinium芯片平台; C: affymetrix-axiom芯片平台。图中每个点为1个基因型数据, 相同基因型的数据点聚集在一起; 两侧红色和蓝色的2组数据点为纯合基因型, 中间1组数据点为杂合基因型。"

附表1

适于玉米分子鉴定的384个SNP位点信息,物理位置参考B73参考基因组AGP-V3版本"

位点
Locus
染色体
Chr.
物理位置
Physical location
等位基因A Allele A 等位基因B Allele B MAF DP PIC AB Rate
MG001 1 2304391 A G 0.49 0.62 0.38 0.51
MG002 1 8974336 A G 0.50 0.62 0.38 0.51
MG003 1 11486897 A G 0.43 0.63 0.37 0.47
MG004 1 17738926 G A 0.41 0.60 0.37 0.53
MG005 1 21466180 C T 0.33 0.60 0.35 0.42
MG006 1 24584752 T G 0.43 0.61 0.37 0.51
MG007 1 27804659 C T 0.39 0.61 0.36 0.47
MG008 1 32232188 G A 0.33 0.60 0.35 0.43
MG009 1 35047741 A G 0.43 0.64 0.37 0.44
MG010 1 39118219 A G 0.32 0.59 0.34 0.42
MG011 1 42927856 T C 0.25 0.54 0.31 0.37
MG012 1 44545185 C A 0.36 0.56 0.36 0.55
MG013 1 51014726 G A 0.47 0.57 0.37 0.58
MG014 1 56566037 C A 0.30 0.58 0.33 0.42
MG015 1 61632511 T C 0.45 0.60 0.37 0.54
MG016 1 67735307 C T 0.50 0.57 0.38 0.59
MG017 1 77806149 C T 0.25 0.54 0.31 0.38
MG018 1 79425465 C T 0.46 0.64 0.37 0.47
MG019 1 92354027 G A 0.35 0.58 0.35 0.49
MG020 1 97688251 C A 0.42 0.62 0.37 0.49
MG021 1 111288396 G A 0.42 0.63 0.37 0.47
MG022 1 121979974 T G 0.38 0.60 0.36 0.49
MG023 1 155443646 G A 0.33 0.58 0.35 0.47
MG024 1 156554418 G T 0.38 0.60 0.36 0.49
MG025 1 161723857 C T 0.32 0.58 0.34 0.47
MG026 1 162755217 A G 0.35 0.59 0.35 0.48
MG027 1 164572994 T C 0.18 0.45 0.25 0.26
MG028 1 171553774 C T 0.34 0.57 0.35 0.51
MG029 1 180966708 T G 0.32 0.57 0.34 0.47
MG030 1 198287528 A G 0.46 0.65 0.37 0.44
MG031 1 200899746 T C 0.45 0.62 0.37 0.51
MG032 1 209627876 G A 0.32 0.59 0.34 0.43
MG033 1 220359715 C T 0.33 0.60 0.34 0.43
MG034 1 243187260 G C 0.30 0.58 0.33 0.43
MG035 1 245753063 A G 0.48 0.59 0.37 0.56
MG036 1 248798195 T C 0.25 0.54 0.31 0.36
MG037 1 253565722 C T 0.30 0.58 0.33 0.43
MG038 1 263219431 G A 0.35 0.60 0.35 0.44
MG039 1 265515707 A C 0.42 0.63 0.37 0.45
MG040 1 269419877 T G 0.26 0.55 0.32 0.41
MG041 1 272938380 A G 0.31 0.56 0.34 0.49
MG042 1 276881002 C T 0.36 0.60 0.35 0.46
MG043 1 277668429 G A 0.41 0.61 0.37 0.49
MG044 1 279336197 T C 0.41 0.62 0.37 0.48
MG045 1 281272643 G A 0.41 0.60 0.37 0.51
MG046 1 282748902 T C 0.45 0.58 0.37 0.56
MG047 1 285846709 C T 0.35 0.61 0.35 0.43
MG048 1 287789543 T C 0.36 0.61 0.35 0.42
MG049 1 289408285 T C 0.46 0.58 0.37 0.57
MG050 1 290379030 T C 0.49 0.64 0.38 0.48
MG051 1 292481023 T C 0.46 0.64 0.37 0.47
MG052 1 296850785 C T 0.33 0.59 0.35 0.46
MG053 1 298863606 C T 0.49 0.62 0.38 0.51
MG054 2 2500805 C T 0.33 0.58 0.35 0.48
MG055 2 3743906 A G 0.49 0.60 0.40 0.55
MG056 2 4697714 C A 0.31 0.56 0.34 0.49
MG057 2 6915357 A G 0.36 0.56 0.36 0.54
MG058 2 8914166 T C 0.46 0.61 0.37 0.53
MG059 2 22258930 G A 0.36 0.57 0.36 0.53
MG060 2 34162472 G A 0.36 0.58 0.35 0.51
MG061 2 47154261 A G 0.40 0.61 0.37 0.50
MG062 2 47789173 T C 0.35 0.60 0.35 0.47
MG063 2 57693847 G A 0.28 0.55 0.32 0.46
MG064 2 109563976 G T 0.34 0.54 0.35 0.56
MG065 2 111835040 C A 0.45 0.65 0.37 0.43
MG066 2 113226924 A C 0.45 0.55 0.37 0.61
MG067 2 145265362 T C 0.47 0.57 0.37 0.59
MG068 2 152240139 G T 0.27 0.56 0.32 0.35
MG069 2 156515220 A C 0.45 0.55 0.37 0.60
MG070 2 160621095 C T 0.47 0.56 0.37 0.60
MG071 2 182623845 G A 0.49 0.57 0.38 0.59
MG072 2 186339948 T G 0.47 0.60 0.37 0.54
MG073 2 191081573 C T 0.48 0.57 0.38 0.59
MG074 2 195937060 C A 0.45 0.62 0.37 0.50
MG075 2 196568843 T G 0.34 0.59 0.35 0.45
MG076 2 199837334 C T 0.27 0.56 0.32 0.40
MG077 2 201108308 C A 0.42 0.61 0.37 0.50
MG078 2 204601534 C T 0.37 0.60 0.36 0.49
MG079 2 207814715 A G 0.37 0.63 0.36 0.37
MG080 2 213018299 G A 0.39 0.61 0.36 0.48
MG081 2 216183437 T C 0.25 0.53 0.30 0.41
MG082 2 219129445 T C 0.27 0.54 0.32 0.44
MG083 2 221291383 C A 0.31 0.59 0.34 0.39
MG084 2 223977328 T G 0.42 0.62 0.37 0.48
MG085 2 225264554 T G 0.25 0.54 0.30 0.38
MG086 2 226199968 A G 0.40 0.61 0.37 0.49
MG087 2 235077065 C T 0.45 0.59 0.37 0.55
MG088 3 8979286 C T 0.37 0.58 0.36 0.51
MG089 3 15222625 G T 0.37 0.62 0.36 0.41
MG090 3 24225424 A G 0.34 0.57 0.35 0.50
MG091 3 25866687 G A 0.30 0.58 0.33 0.39
MG092 3 27775731 T C 0.47 0.54 0.37 0.63
MG093 3 29213820 T G 0.46 0.62 0.37 0.51
MG094 3 54969279 G T 0.49 0.59 0.38 0.56
MG095 3 57091132 C A 0.31 0.58 0.34 0.44
MG096 3 58082419 A G 0.30 0.56 0.33 0.47
MG097 3 67702516 G A 0.43 0.63 0.37 0.47
MG098 3 92555979 T C 0.36 0.59 0.35 0.50
MG099 3 88400489 C T 0.46 0.63 0.37 0.48
MG100 3 117106253 C A 0.37 0.61 0.36 0.46
MG101 3 118083714 C A 0.49 0.51 0.38 0.66
MG102 3 122901197 C T 0.49 0.61 0.38 0.52
MG103 3 126128781 C T 0.44 0.57 0.37 0.57
MG104 3 132489439 C T 0.46 0.62 0.37 0.51
MG105 3 134796979 A C 0.45 0.60 0.37 0.54
MG106 3 141646415 C T 0.50 0.63 0.38 0.50
MG107 3 142724956 T C 0.30 0.55 0.33 0.48
MG108 3 151086172 T C 0.40 0.61 0.36 0.50
MG109 3 152533264 A G 0.36 0.59 0.35 0.48
MG110 3 155358367 G A 0.34 0.61 0.35 0.42
MG111 3 156325759 A G 0.41 0.59 0.37 0.53
MG112 3 161301281 A G 0.48 0.63 0.38 0.49
MG113 3 162340416 G A 0.39 0.62 0.36 0.46
MG114 3 169261670 A G 0.48 0.65 0.38 0.45
MG115 3 173048348 T C 0.40 0.63 0.37 0.45
MG116 3 174581037 T C 0.33 0.58 0.35 0.48
MG117 3 179842209 A G 0.49 0.61 0.38 0.53
MG118 3 181032542 T C 0.34 0.60 0.35 0.43
MG119 3 184176645 T G 0.36 0.59 0.36 0.50
MG120 3 196000794 C T 0.44 0.62 0.37 0.49
MG121 3 200262266 T C 0.38 0.62 0.36 0.45
MG122 3 201757644 G A 0.49 0.60 0.38 0.54
MG123 3 203824251 A C 0.42 0.61 0.37 0.51
MG124 3 206611097 G A 0.30 0.58 0.33 0.37
MG125 3 208142658 T C 0.35 0.61 0.35 0.41
MG126 3 209109183 T G 0.30 0.59 0.33 0.38
MG127 3 212050055 A C 0.46 0.57 0.37 0.58
MG128 3 218112568 T C 0.44 0.62 0.37 0.50
MG129 3 221653490 A C 0.26 0.55 0.31 0.34
MG130 3 226343075 A G 0.46 0.62 0.37 0.50
MG131 3 229397149 T G 0.38 0.63 0.36 0.41
MG132 4 1487851 G A 0.26 0.55 0.31 0.37
MG133 4 14131426 A G 0.42 0.58 0.37 0.56
MG134 4 18832691 A C 0.41 0.60 0.37 0.51
MG135 4 20077010 G T 0.39 0.59 0.36 0.51
MG136 4 20761638 A C 0.21 0.49 0.27 0.31
MG137 4 22576276 T C 0.28 0.56 0.32 0.41
MG138 4 31743151 T G 0.30 0.58 0.33 0.40
MG139 4 33150343 T C 0.45 0.65 0.37 0.40
MG140 4 37381509 A T 0.33 0.61 0.34 0.37
MG141 4 39828005 T C 0.39 0.62 0.36 0.46
MG142 4 45151079 C T 0.47 0.60 0.37 0.55
MG143 4 50184084 T G 0.35 0.57 0.35 0.52
MG144 4 53473032 A G 0.41 0.60 0.37 0.51
MG145 4 55889650 C T 0.49 0.64 0.38 0.47
MG146 4 67519590 T C 0.48 0.63 0.37 0.49
MG147 4 77234543 A G 0.49 0.62 0.38 0.52
MG148 4 128874466 G T 0.33 0.58 0.35 0.47
MG149 4 145650338 A G 0.35 0.58 0.35 0.51
MG150 4 149311096 G A 0.39 0.64 0.36 0.35
MG151 4 151253200 A G 0.37 0.62 0.36 0.44
MG152 4 153661937 G A 0.44 0.64 0.37 0.45
MG153 4 154880065 C T 0.41 0.61 0.37 0.51
MG154 4 164294138 C T 0.30 0.56 0.33 0.47
MG155 4 169994302 G A 0.49 0.63 0.38 0.49
MG156 4 173238441 A G 0.31 0.59 0.34 0.39
MG157 4 177180130 A G 0.46 0.58 0.37 0.57
MG158 4 179824742 C T 0.48 0.61 0.38 0.53
MG159 4 184328972 G A 0.26 0.55 0.31 0.38
MG160 4 193937034 T C 0.39 0.59 0.36 0.52
MG161 4 201638107 A G 0.41 0.62 0.37 0.47
MG162 4 208661194 T C 0.32 0.58 0.34 0.46
MG163 4 215441456 A G 0.32 0.58 0.34 0.45
MG164 4 221839256 G T 0.34 0.61 0.35 0.40
MG165 4 223648151 G A 0.45 0.61 0.37 0.51
MG166 4 230935341 T G 0.40 0.58 0.37 0.55
MG167 4 232019010 A C 0.40 0.59 0.36 0.52
MG168 4 234081042 G T 0.31 0.56 0.34 0.50
MG169 4 236715495 C T 0.42 0.62 0.37 0.49
MG170 4 238918388 G A 0.35 0.61 0.35 0.43
MG171 4 240891845 T G 0.39 0.58 0.36 0.53
MG172 5 6881507 A G 0.40 0.63 0.37 0.45
MG173 5 8507286 C T 0.33 0.60 0.34 0.40
MG174 5 11356449 T C 0.32 0.56 0.34 0.51
MG175 5 13402375 T C 0.45 0.60 0.37 0.54
MG176 5 15452020 C T 0.27 0.55 0.32 0.43
MG177 5 17881372 G A 0.49 0.64 0.38 0.47
MG178 5 21008784 T C 0.39 0.61 0.36 0.47
MG179 5 29937148 C T 0.42 0.63 0.37 0.44
MG180 5 31668063 C T 0.48 0.65 0.37 0.44
MG181 5 33072014 G A 0.43 0.58 0.37 0.56
MG182 5 35669253 G A 0.29 0.57 0.33 0.40
MG183 5 38831562 T C 0.17 0.44 0.25 0.24
MG184 5 48413218 G T 0.47 0.63 0.37 0.50
MG185 5 59068812 T C 0.48 0.63 0.38 0.48
MG186 5 60955626 C T 0.37 0.59 0.36 0.51
MG187 5 62137456 A G 0.26 0.54 0.31 0.40
MG188 5 64134193 G A 0.33 0.57 0.34 0.49
MG189 5 72721863 C T 0.31 0.58 0.33 0.41
MG190 5 74941891 A G 0.27 0.56 0.32 0.39
MG191 5 80839340 G A 0.45 0.60 0.37 0.54
MG192 5 86070543 C T 0.33 0.57 0.34 0.49
MG193 5 88226330 G A 0.40 0.61 0.37 0.48
MG194 5 93618116 A G 0.30 0.57 0.33 0.43
MG195 5 130860915 T C 0.48 0.62 0.37 0.51
MG196 5 141206885 C T 0.44 0.65 0.37 0.42
MG197 5 161040798 G T 0.30 0.58 0.33 0.39
MG198 5 163451299 C T 0.37 0.63 0.36 0.38
MG199 5 166437548 T C 0.38 0.63 0.36 0.40
MG200 5 173209693 G A 0.38 0.62 0.36 0.44
MG201 5 174352063 A G 0.46 0.63 0.37 0.49
MG202 5 176085344 G A 0.43 0.59 0.37 0.55
MG203 5 178878084 T C 0.42 0.64 0.37 0.42
MG204 5 181267545 A G 0.36 0.60 0.36 0.48
MG205 5 190533458 G A 0.48 0.64 0.38 0.47
MG206 5 199812574 T C 0.49 0.61 0.38 0.53
MG207 5 204879476 G A 0.39 0.58 0.36 0.54
MG208 5 215521126 T C 0.35 0.62 0.35 0.39
MG209 5 217664096 C T 0.49 0.62 0.38 0.51
MG210 6 3592433 A G 0.45 0.61 0.37 0.52
MG211 6 4843861 T C 0.40 0.63 0.37 0.43
MG212 6 6530602 G A 0.38 0.61 0.36 0.47
MG213 6 8111698 A C 0.35 0.59 0.35 0.49
MG214 6 33247659 G A 0.48 0.64 0.37 0.48
MG215 6 39822979 C T 0.50 0.55 0.38 0.62
MG216 6 42757005 T G 0.48 0.60 0.38 0.54
MG217 6 45872006 G T 0.37 0.60 0.36 0.49
MG218 6 53883513 T G 0.27 0.56 0.32 0.36
MG219 6 56274915 G A 0.25 0.53 0.30 0.42
MG220 6 56968490 C G 0.36 0.60 0.36 0.47
MG221 6 61133030 G T 0.49 0.62 0.40 0.53
MG222 6 80804393 C T 0.37 0.58 0.36 0.52
MG223 6 83130638 G A 0.50 0.64 0.38 0.47
MG224 6 86421059 T A 0.32 0.57 0.34 0.49
MG225 6 93160349 A G 0.40 0.59 0.37 0.53
MG226 6 110465918 C T 0.50 0.66 0.38 0.41
MG227 6 111230603 C T 0.40 0.61 0.37 0.49
MG228 6 108233804 A T 0.37 0.62 0.36 0.43
MG229 6 110053930 G A 0.49 0.63 0.38 0.49
MG230 6 114361855 C T 0.47 0.59 0.37 0.55
MG231 6 118325927 C A 0.44 0.61 0.37 0.51
MG232 6 120823677 A G 0.33 0.60 0.34 0.39
MG233 6 123789176 C T 0.36 0.59 0.35 0.48
MG234 6 125370735 G A 0.42 0.58 0.37 0.56
MG235 6 132222944 T C 0.31 0.57 0.34 0.45
MG236 6 133537579 C T 0.48 0.62 0.37 0.51
MG237 6 134282494 C T 0.38 0.61 0.36 0.48
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MG279 7 155821714 A G 0.42 0.61 0.37 0.50
MG280 7 157609773 A C 0.35 0.62 0.35 0.34
MG281 7 158991658 C T 0.45 0.65 0.37 0.42
MG282 7 162196275 A G 0.37 0.57 0.36 0.54
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MG284 7 169245453 C T 0.48 0.64 0.37 0.47
MG285 7 172200651 T C 0.36 0.57 0.36 0.53
MG286 7 175252105 G A 0.43 0.65 0.37 0.38
MG287 8 2761824 G A 0.43 0.62 0.37 0.48
MG288 8 4177172 G T 0.48 0.59 0.38 0.56
MG289 8 7624059 T G 0.28 0.56 0.32 0.43
MG290 8 10124026 T C 0.48 0.63 0.38 0.49
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MG297 8 65658110 A C 0.29 0.55 0.33 0.47
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MG301 8 77266692 C T 0.37 0.62 0.36 0.42
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MG304 8 85385460 T C 0.44 0.60 0.37 0.54
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MG351 9 148627045 C T 0.49 0.56 0.38 0.60
MG352 9 149308774 A C 0.49 0.65 0.38 0.42
MG353 9 150911119 G A 0.45 0.58 0.37 0.56
MG354 9 151542506 A G 0.49 0.61 0.38 0.53
MG355 9 155054804 G A 0.32 0.57 0.34 0.48
MG356 10 2802838 T G 0.43 0.62 0.37 0.48
MG357 10 10674490 C T 0.21 0.49 0.27 0.35
MG358 10 22133988 C T 0.30 0.57 0.33 0.43
MG359 10 32194186 T C 0.30 0.56 0.33 0.47
MG360 10 58207909 T C 0.45 0.64 0.37 0.45
MG361 10 39960289 C T 0.48 0.59 0.40 0.57
MG362 10 61011906 G A 0.49 0.65 0.38 0.45
MG363 10 76655433 T G 0.50 0.66 0.38 0.40
MG364 10 82039366 G A 0.48 0.61 0.38 0.53
MG365 10 84110544 T G 0.33 0.59 0.35 0.44
MG366 10 89433313 G A 0.36 0.60 0.36 0.46
MG367 10 95929973 A G 0.46 0.59 0.37 0.55
MG368 10 103380111 A G 0.47 0.62 0.37 0.50
MG369 10 109396730 G A 0.49 0.61 0.38 0.53
MG370 10 114217715 G A 0.49 0.64 0.38 0.46
MG371 10 117773469 T G 0.38 0.60 0.36 0.50
MG372 10 121559630 G A 0.50 0.63 0.38 0.50
MG373 10 129486606 A G 0.30 0.58 0.33 0.39
MG374 10 130399835 A G 0.32 0.58 0.34 0.45
MG375 10 131065538 T C 0.43 0.65 0.37 0.42
MG376 10 131912401 T C 0.49 0.65 0.38 0.44
MG377 10 132535283 G A 0.28 0.55 0.32 0.45
MG378 10 133905339 G A 0.28 0.57 0.32 0.36
MG379 10 135351702 C T 0.35 0.61 0.35 0.41
MG380 10 135937058 T C 0.32 0.59 0.34 0.41
MG381 10 137192651 A C 0.42 0.62 0.37 0.48
MG382 10 139565935 A G 0.41 0.59 0.37 0.54
MG383 10 144384643 A C 0.49 0.64 0.38 0.47
MG384 10 147014927 G A 0.29 0.57 0.33 0.41

图2

maizeSNP384位点组合的分布 A: 384个SNP位点在玉米10对染色体上分布图; B: 384个SNP位点在基因上的分布情况。"

图3

384个SNP位点的MAF和PIC多态性指数变化分布图 横坐标为MAF、PIC的区间值, 纵坐标为SNP位点数目。"

图4

335个玉米杂交种SNP-DNA指纹总体展示图 每行代表1个品种, 按照国家审定编号排序; 每列代表1个SNP位点, 按照在基因组上物理位置排序; DNA指纹分别用不同的颜色表示。"

图5

335个玉米品种的杂合基因型频率、遗传差异分析 A: 335个玉米品种的杂合基因型频率分布(杂合基因型的标记数目/384); B: 335个玉米品种两两成对比较差异位点数分布图, 横坐标为成对比较差异位点数目, 纵坐标为成对比较的数目; C: 335个玉米品种两两成对比较遗传相似系数分布图, 横坐标为遗传相似系数百分比, 纵坐标为成对比较的数目。"

图6

基于最优位点组合的品种识别率变化曲线图"

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