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作物学报 ›› 2010, Vol. 36 ›› Issue (3): 385-390.doi: 10.3724/SP.J.1006.2010.00385

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

黄淮冬麦区小麦冬、春性改良及分子标记辅助选择技术初探

张晶1，吴锁伟1，刘秉华1，宋梅芳2，周朋1，郭春燕3，詹克慧3，王山荭1，杨丽1，董冬1，于立强4，李辉利4，周阳1*，杨建平1*   

  1. 1中国农业科学院作物科学研究所/农作物基因资源与基因改良重大科学工程，北京100081；2山东农业科学院蔬菜研究所，山东济南250100；3河南农业大学农学院，河南郑州450002；4河北省赵县农业科学研究所，河北石家庄051530
  • 收稿日期:2009-10-09 修回日期:2010-01-08 出版日期:2010-03-12 网络出版日期:2010-01-22
  • 通讯作者: 周阳, E-mail: zhouyang@mail.caas.net.cn, Tel: 010-82108628; 杨建平, E-mail: yangjianping@caas.net.cn, Tel: 010-82105859
  • 基金资助:

    本研究由国家高科技研究发展计划（863计划）项目（2006AA10Z1F2）和国家重点基础研究发展计划（973计划）项目（2009CB118300）资助。

Genetic Improvement of Wheat Growth Habit and Its Molecular Marker-Assisted Selection in Yellow and Huai River Reaches

ZHANG Jing1, WU Suo-Wei1, LIU Bing-Hua1, SONG Mei-Fang2, ZHOU Peng1, GUO Chun-Yan3, ZHAN Ke-Hui3, WANG Shan-Hong1, YANG Li1, DONG Dong1, YU Li-Qiang4, LI Hui-Li4, ZHOU Yang1,*, and YANG Jian-Ping1,*   

  1. 1Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2Vegetable Research Institute,Shandong Academy of Agricultural sciences,Jinan 250100,China;3College of Agronomy,Henan Agricultural University,Zhengzhou 450002,China;4Zhaoxian Agricultural Research Institute of Hebei Province,Shijiazhuang 051530.China
  • Received:2009-10-09 Revised:2010-01-08 Published:2010-03-12 Published online:2010-01-22
  • Contact: ZHOU Yang,E-mail: zhouyang@mail.caas.net.cn, Tel: 010-82108628; YANG Jian-ping, E-mail: yangjianping@caas.net.cn, Tel: 010-82105859

PDF

1789

被引次数

7

摘要：

通过对小麦品种石麦12春化特性的遗传和分子标记研究，探索黄淮冬麦区小麦冬、春性改良途径和分子标记辅助选择技术。石麦12与冬性品种石家庄8号杂交后代F2:3株系中的春性株系、冬春性分离株系、冬性株系的分离比例符合1∶2∶1，表明石麦12具有一个显性春化基因，经已知春化基因的基因特异性标记鉴定为Vrn-D1。利用Vrn-D1的基因特异性标记对上述F2:3株系进行冬、春性鉴定的结果与表型鉴定结果一致，说明该分子标记可用于小麦冬、春性改良中对Vrn-D1的辅助选择。在高海拔、长日照地区夏播是小麦冬、春性表型鉴定的一个快速、简便途径。

关键词: 小麦育种, 春化基因, 分子标记

Abstract:

The vernalizatioin gene in wheat cultivar Shimai 12 (Triticum aestivum L.) was investigated to find a more efficient way for the improvement of growth habit of wheat cultivars grown in Yellow and Huai River Reaches. In the F2:3 lines derived from the cross between Shimai 12 and Shijiazhuang 8 (a winter wheat cultivar), the ratio of the spring, segregating, and winter lines was in accordance to 1:2:1 based on chi-square test, showing that the spring growth habit of Shimai 12 was determined by single dominant gene, which was identified as Vrn-D1 by gene-specific markers. The Vrn-D1 gene-specific marker was further used to genotype the F2:3 lines, and the results were consistent with phenotyping, verifying the available utilization of the marker assisted selection for Vrn-D1 in breeding programs. Besides, summer sowing in high altitude and cool area proved to be a cost-saving and efficient method for wheat growth habit identification.

Key words: Wheat breeding, Vernalization gene, Molecular marker

[1] Zhai P M, Chao Q C, Zou X K. Progress in China’s climate change study in the 20th century. J Geogr Sci, 2004, 14(suppl): 3-11



[2] Shi P-J(史培军), Wang J-A(王静爱), Xie Y(谢云), Wang P(王平), Zhou W-G(周武光). A preliminary study on the climatic change, natural disasters of agriculture and grain yield in China in the past 15 years. J Nat Resour (自然资源学报), 1997, 12(3): 197-203 (in Chinese with English abstract)



[3] Yang Z-Q(杨宗渠), Yin J(尹钧), Zhou R(周冉), Li J-C(李金才). Study on vernalization character of different genotypes of wheat from Huanghuai wheat production area. J Triticeae Crops (麦类作物学报), 2006, 26(2): 82-85 (in Chinese with English abstract)



[4] Li M-S(李茂松), Wang D-L(王道龙), Zhang Q(张强), Chi Y-G(迟永刚), Wang C-Y(王春艳), Kiribuchi C O, Yoshida H. Cold damage of autumn sown wheat in Huang-Huai-Hai River reaches in 2004-2005 growing season. J Nat Disasters (自然灾害学报), 2005, 14(4): 51-55 (in Chinese)



[5] Distelfeld A, Li C, Dubcovsky J. Regulation of flowering in temperate cereals. Curr Opin Plant Biol, 2009, 12: 1-7



[6] Zhang X K, Xiao Y G, Zhang Y, Xia X C, Dubcovsky J, He Z H. Allelic variation at the vernalization genes Vrn-A1, Vrn-B1, Vrn-D1 and Vrn-B3 in Chinese wheat cultivars and their association with growth habit. Crop Sci, 2008, 48: 458-470



[7] Trevaskis B, Hemming M N, Dennis E S, Peacock J. The molecular basis of vernalization-induced flowering in cereals. Trends Plant Sci, 2007, 12: 352-357



[8] Greenup A, Peacock W J, Dennis E S, Trevaskis B. The molecular biology of seasonal flowering-responses in Arabidopsis and the cereals. Ann Bot (Lond), 2009, 103: 1165-1172



[9] Dubcovsky J, Loukoianov A, Fu D L, Valarik M, Sanchez A, Yan L L. Effect of photoperiod on the regulation of wheat vernalization genes VRN1 and VRN2. Plant Mol Biol, 2006, 60: 469-480



[10] Yan L L, Loukoianov A, Tranquilli G, Helguera M, Fahima T, Dubcovsky J. Positional cloning of the wheat vernalization gene VRN1. Proc Natl Acad Sci USA, 2003, 100: 6263-6368



[11] Dubcovsky J, Lijavetzky D, Appendino L, Tranquilli G. Comparative RFLP mapping of Triticum monococcum genes controlling vernalization requirement. Theor Appl Genet, 1998, 97: 968-975



[12] Law C N, Worland A J, Giorgi B. The genetic control of ear-emergence time by chromosomes 5A and 5D of wheat. Heredity, 1975, 36: 49-58



[13] Galiba G, Quarrie S A, Sutka J, Morgounov A, Snape J W. RFLP mapping of the vernalization (Vrnl) and frost resistance (Frl) genes on chromosome 5A of wheat. Theor Appl Genet, 1995, 90: 1174-1179



[14] Iwaki K, Nishida J, Yanagisawa T, Yoshida H, Kato K. Genetic analysis of Vrn-B1 for vernalization requirement by using linked dCAPS markers in bread wheat (Triticum aestivum L.). Theor Appl Genet, 2002, 104: 571-576



[15] Barrett B, Bayram M, Kidwell K. Identifying AFLP and microsatellite markers for vernalization response gene Vrn-B1 in hexaploid wheat using reciprocal mapping populations. Plant Breed, 2002,121:400-406



[16] Yan L L, Fu D L, Li C, Blechl A, Tranquilli G, Bonafede M, Sanchez A, Valarik M, Yasuda S, Dubcovsky J. The wheat and barley vernalization gene VRN3 is an orthologue of FT. Proc Natl Acad Sci USA, 2006, 103: 19581-19586



[17] Fu D L, Szucs P, Yan L L, Helguera M, Skinner J S, Zitzewitz J V, Hayes P M, Dubcovsky J. Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat. Mol Genet Genomics, 2005, 273: 54-65



[18] Yan L L, Helguera M, Kato K, Fukuyama S, Sherman J, Dubcovsky J. Allelic variation at the VRN-1 promoter region in polyploidy wheat. Theor Appl Genet, 2004, 109: 1677-1686



[19] Chen D H, Ronald P C. A rapid DNA minipreparation method suitable for AFLP and other PCR applications. Plant Mol Biol Rep, 1999, 17: 53-57



[20] Pugsley A T. The impact of plant physiology on Australian wheat breeding. Euphytica, 1983, 32: 743-748



[21] Sun Q M, Zhou R H, Gao L F, Zhao G Y, Jia J Z. The characterization and geographical distribution of the genes responsible for vernalization requirement in Chinese bread wheat. J Integr Plant Biol, 2009, 51: 423-432
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