作物学报 ›› 2018, Vol. 44 ›› Issue (02): 169-176.doi: 10.3724/SP.J.1006.2018.00169
严贤诚**, 陈立凯**, 罗玉花, 罗文龙, 王慧, 郭涛*(), 陈志强*()
Xian-Cheng YAN**, Li-Kai CHEN**, Yu-Hua LUO, Wen-Long LUO, Hui WANG, Tao GUO*(), Zhi-Qiang CHEN*()
摘要:
水稻的花器官发育影响着水稻的产量与品质。本研究通过12C重离子诱变航恢7号获得一个水稻花器官突变体multi-floret 2 (mf2), 其稃片、浆片、雄蕊、雌蕊增多, 多数小穗内具2~3朵类似小花。mf2内外稃不能很好勾合, 而且形状和维管束的数目都产生了一定程度的变化。电镜扫描幼穗发现花器官的变异在幼穗分化期的各花器官原基分化时就已形成。另外, 该突变体的抽穗期推迟, 株高降低, 穗数增多, 表明其营养生长也受到一定的影响。遗传分析表明mf2突变体表型受单隐性核基因控制。利用SSR、InDel分子标记将MF2定位于第1染色体的标记SSR39108和InD39210之间, 区间大小约为102 kb。
[1] | Guo S, Sun B, Looi L S, Xu Y, Gan E S, Huang J, Ito T.Co-ordination of flower development through epigenetic regulation in two model species: rice andArabidopsis. Plant Cell Physiol, 2015, 56: 830-842 |
[2] | Yanofsky M F.Floral meristems to floral organs: genes controlling early events in Arabidopsis flower development.Annu Rev Plant Physiol Plant Mol Biol, 1995, 46: 167-188 |
[3] | Fornara F, Marziani G, Mizzi L, Kater M, Colombo L.MADS-box genes controlling flower development in rice.Plant Biol, 2003, 5: 16-22 |
[4] | 田大刚, 刘华清, 苏军, 张礼华, 王锋. 水稻与拟南芥中控制花器官发育MADS-box基因的比较研究进展. 福建农业学报, 2011, 26: 309-320 |
Tian D G, Liu H Q, Su J, Zhang L H, Wang F.Flower-Development-Controlling MADS-box genes in rice andArabidopsis thaliana. Fujian J Agric Sci, 2011, 36: 309-320 (in Chinese with English abstract) | |
[5] | Yoshida H, Nagato Y.Flower development in rice.J Exp Bot, 2011, 62: 4719-4730 |
[6] | Takahashi M, Nagasawa N, Kitano H, Nagato Y.Panicle phytomer 1 mutations affect the panicle architecture of rice. Theor Appl Genet, 1998, 96: 1050-1056 |
[7] | Kyozuka J, Konishi S, Nemoto K, Izawa T, Shimamoto K.Down-regulation ofRFL, the FLO/LFY homolog of rice, accompanied with panicle branch initiation. Proc Natl Acad Sci USA, 1998, 95: 1979-1982 |
[8] | Jeon J S, Jang S, Lee S, Nam J, Kim C, Lee S H, Chung Y Y, Kim S R, Lee Y H, Cho Y G.Leafy hull sterile 1 is a homeotic mutation in a rice MADS box gene affecting rice flower development. Plant Cell, 2000, 12: 871-884 |
[9] | Chanhong K, Donghoon J, An G H.Molecular cloning and characterization ofOsLRK1 encoding a putative receptor-like protein kinase from Oryza sativa. Plant Sci, 2000, 152: 17-26 |
[10] | Jang S, Lee B, Kim C, Kim S J, Yim J, Han J J, Lee S, Kim S R, An G.TheOsFOR1 gene encodes a polygalacturonase-inhibiting protein (PGIP) that regulates floral organ number in rice. Plant Mol Biol, 2003, 53: 357-369 |
[11] | Suzaki T, Sato M, Ashikari M, Miyoshi M, Nagato Y, Hirano H Y.The geneFLORAL ORGAN NUMBER1 regulates floral meristern size in rice and encodes a leucine-rich repeat receptor kinase orthologous to Arabidopsis CLAVATA1. Development, 2004, 131: 5649-5657 |
[12] | Chu H, Qian Q, Liang W, Yin C, Tan H, Yao X, Yuan Z, Yang J, Huang H, Luo D.TheFLORAL ORGAN NUMBER4 gene encoding a putative ortholog of Arabidopsis CLAVATA3 regulates apical meristem size in rice. Plant Physiol, 2006, 142: 1039-1052 |
[13] | Suzaki T, Toriba T, Fujimoto M, Tsutsumi N, Kitano H, Hirano H Y.Conservation and diversification of meristem maintenance mechanism inOryza sativa: function of the FLORAL ORGAN NUMBER2 gene. Plant Cell Physiol, 2006, 47: 1591-1602 |
[14] | Zhao L, Xu S, Chai T, Tai W.OsAP2-1, an AP2-like gene from Oryza sativa, is required for flower development and male fertility. Plant Reprod, 2006, 19: 197-206 |
[15] | Lee D Y, Lee J, Moon S, Park S Y, An G.The rice heterochronic geneSUPERNUMERARY BRACT regulates the transition from spikelet meristem to floral meristem. Plant J, 2007, 49: 64-78 |
[16] | Sun Q, Zhou D X.Rice jmjC domain-containing geneJMJ706 encodes H3K9 demethylase required for floral organ development. Proc Natl Acad Sci USA, 2008, 105: 13679-13684 |
[17] | Li H, Xue D, Gao Z, Yan M, Xu W, Xing Z, Huang D, Qian Q, Xue Y.A putative lipase geneEXTRA GLUME1 regulates both empty-glume fate and spikelet development in rice. Plant J Cell Mol Biol, 2009, 57: 593-605 |
[18] | Xiao H, Tang J, Li Y, Wang W, Li X, Jin L, Xie R, Luo H, Zhao X, Meng Z.STAMENLESS 1, encoding a single C2H2 zinc finger protein, regulates floral organ identity in rice. Plant J, 2009, 59: 789-801 |
[19] | Ren D Y, Li Y F, Wang Z, Xu F F, Guo S, Zhao F M, Sang X C, Ling Y H, He G H.Identification and gene mapping of amulti-floret spikelet 1 (mfsl) mutant associated with spikelet development in rice. J Integr Agric, 2012, 11: 1574-1579 |
[20] | Wang N, Li Y F, Sang X C, Ling Y H, Zhao F M, Yang Z L, He G H.Nonstop glumes (nsg), a novel mutant affects spikelet development in rice. Genes & Genomics, 2013, 35: 149-157 |
[21] | Cai Q, Yuan Z, Chen M, Yin C, Luo Z, Zhao X, Liang W, Hu J, Zhang D.Jasmonic acid regulates spikelet development in rice.Nat Commun, 2014, 5: 3476 |
[22] | Zhang J, Tang W, Huang Y, Niu X, Zhao Y, Han Y, Liu Y.Down-regulation of a LBD-like gene,OsIG1, leads to occurrence of unusual double ovules and developmental abnormalities of various floral organs and megagametophyte in rice. J Exp Bot, 2015, 66: 99-112 |
[23] | Wang H H, Zhang L, Cai Q, Jin Z M, Zhao X X, Huang Q M, Luo Z J, Chen M J, Zhang D B, Yuan Z.OsMADS32 interacts with PI-like proteins and regulates rice flower development. J Integr Plant Biol, 2015, 57: 504-513 |
[24] | Yang C, Ma Y, Li J.The riceYABBY4 gene regulates plant growth and development through modulating the gibberellin pathway. J Exp Bot, 2016, 67: 5545-5556 |
[25] | 李云峰, 杨正林, 凌英华, 王楠, 任德勇, 王增, 何光华. 水稻多小花小穗突变体mf1的鉴定与基因定位. 作物学报, 2011, 37: 280-285 |
Li Y F, Yang Z L, Ling Y H, Wang N, Ren D Y, Wang Z, He G H.Characterization and gene mapping of a spikelet mutantmf1 in rice. Acta Agron Sin, 2011, 37: 280-285 (in Chinese with English abstract) | |
[26] | Murray M G, Thompson W F.Rapid isolation of high molecular weight plant DNA.Nucl Acids Res, 1980, 8: 4321-4326 |
[27] | Michelmore R W, Paran I, Kesseli R V.Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations.Proc Natl Acad Sci USA, 1991, 88: 9828-9832 |
[28] | 赵利峰, 柴团耀. AP2/EREBP转录因子在植物发育和胁迫应答中的作用. 植物学通报, 2008, 25: 89-101 |
Zhao L F, Chai T Y.Roles of AP2/EREBP family of transcription factors in development and stress response of plants,Chin Bull Bot, 2008, 25: 89-101 (in Chinese with English abstract) | |
[29] | Poethig R S.Phase change and the regulation of developmental timing in plants.Science, 2003, 301: 334-336 |
[30] | Feng L, Gao Z, Xiao G, Huang R, Zhang H.Leucine-rich repeat receptor-like kinaseFON1 regulates drought stress and seed germination by activating the expression of ABA-responsive fenes in rice. Plant Mol Biol Rep, 2014, 32: 1158-1168 |
[31] | Jiang L, Qian Q, Mao L, Zhou Q Y, Zhai W X.Characterization of the rice floral organ number mutantfon3. J Integr Plant Biol, 2005, 47: 100-106 |
[32] | Li Y, Xu P, Zhang H, Peng H, Zhang Q, Wang X, Wu X.Characterization and identification of a novel mutantfon(t) on floral organ number and floral organ identity in rice. J Genet Genomics, 2007, 34: 730-737 |
[33] | Kaplinsky N J, Freeling M.Combinatorial control of meristem identity in maize inflorescences.Development, 2003, 130: 1149-1158 |
[34] | Lee D Y, An G.Two AP2 family genes,SUPERNUMERARY BRACT (SNB) and OsINDETERMINATE SPIKELET 1 (OsIDS1), synergistically control inflorescence architecture and floral meristem establishment in rice. Plant J, 2012, 69: 445-461 |
[35] | Padham A K, Hopkins M T, Wang T W, Mcnamara L M, Lo M, Richardson L G, Smith M D, Taylor C A, Thompson J E.Characterization of a plastid triacylglycerol lipase fromArabidopsis. Plant Physiol, 2007, 143: 1372-1384 |
[36] | Zhang B, Wu S, Zhang Y, Xu T, Guo F, Tang H, Li X, Wang P, Qian W, Xue Y.A high temperature-dependent mitochondrial lipaseEXTRA GLUME1 promotes floral phenotypic robustness against temperature fluctuation in rice(Oryza sativa L.). PLoS Genet, 2016, 12: e1006152 |
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