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作物学报 ›› 2018, Vol. 44 ›› Issue (9): 1393-1399.doi: 10.3724/SP.J.1006.2018.01393

• 研究论文 • 上一篇    下一篇

外源NO供体对水分亏缺下玉米叶片碳同化关键酶及抗氧化系统的影响

杨青华(),郑博元,李蕾蕾,贾双杰,韩心培,郭家萌,王泳超,邵瑞鑫()   

  1. 河南农业大学农学院, 河南郑州450046
  • 收稿日期:2017-12-22 接受日期:2018-06-12 出版日期:2018-09-10 网络出版日期:2018-07-02
  • 通讯作者: 邵瑞鑫
  • 基金资助:
    本研究由国家自然科学基金项目(31401304)资助

Effect of Exogenous Nitric Oxide Donor on Carbon Assimilation and Antioxidant System in Leaves of Maize Seedlings under PEG-induced Water Deficit Stress

Qing-Hua YANG(),Bo-Yuan ZHENG,Lei-Lei LI,Shuang-Jie JIA,Xin-Pei HAN,Jia-Meng GUO,Yong-Chao WANG,Rui-Xin SHAO()   

  1. College of Agronomy, Henan Agricultural University, Zhengzhou 450046, Henan, China
  • Received:2017-12-22 Accepted:2018-06-12 Published:2018-09-10 Published online:2018-07-02
  • Contact: Rui-Xin SHAO
  • Supported by:
    This study was supported by the National Natural Science Foundation of China (31401304)

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摘要：

为了探讨外源NO供体(硝普钠, SNP)对水分亏缺下玉米叶片碳同化关键酶及抗氧化系统的影响及其调控机制, 在20% PEG-6000模拟水分亏缺胁迫下, 研究了SNP对玉米品种驻玉309幼苗叶片光合碳同化核酮糖-1,5-二磷酸羧化/加氧酶(Rubisco)和Rubisco活化酶(RCA)活性及其基因表达、抗氧化酶活性及其同工酶谱变化的影响。结果表明, 在水分亏缺胁迫下, SNP显著上调玉米叶片rbc L、rbc S、rca β基因的相对表达量, 尤其是叶片rbc S基因的相对表达量增加1.86倍, 叶片Rubisco、RCA活性分别提高32.7%和14.67%; 叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性及其同工酶谱带的宽度和亮度显著增强, 而ROS积累量明显降低。说明在PEG水分亏缺胁迫下, SNP能显著提升玉米幼苗叶片光合碳同化能力及抗氧化酶活性, 降低ROS积累及其对细胞膜造成的损伤, 提高玉米的抗干旱性。

关键词: 外源一氧化氮, 水分亏缺, 玉米幼苗, 光合碳同化, 抗氧化系统同工酶

Abstract:

The objective of this study was to explore the effect of exogenous nitric oxide (NO) donor (sodium nitroprusside, SNP) on key enzymes of carbon assimilation and antioxidant system of maize leaves under water deficit and its regulation mechanism. In this experiment, 20% PEG-6000 was used to stimulate water deficit stress, exogenous SNP was added into root rhizosphere of seedlings in maize variety Zhuyu 309. After three days of stresses, the changes of Rubisco and RCA activities and their gene level, antioxidase activity and their isoenzyme spectrum level were investigated. The expression levels of rbc L, rbc S, rca β were increased significantly, especially for rbc S that was increased the most by 1.86 fold, which resulted in up-regulation of Rubisco and RCA activities by 32.7% and 14.67% under exogenous SNP plus PEG stress. In addition, SNP enhanced the activity of SOD, POD, CAT, and the width in their isoenzyme spectrum, resulting in significant reduction of ROS accumulation. These results suggested that NO could increase photosynthetic carbon assimilation capacity and antioxidase activity, alleviate the damage of ROS burst on the cell membrane, which enhances PEG-simulated water deficit resistance of maize seedlings.

Key words: exogenous nitric oxide, water deficit, maize seedlings, carbon assimilation, antioxidant system

表1

本实验引物序列"

引物名称
Primer name		 序列
Sequence (5'-3')		 Tm
(°C)
actin-F CTGAACCCCAAGGCAAACA 59.0
actin-R ACTGGCGTACAGGGAAAGAA 57.3
rca β-F TCCTTGAGACCTTCTTGACGG 59.8
rca β-R ATCGCCTTGAACCTGCTGT 57.8
rbc L-F CCGTTTCGTCTTTTGTGCC 58.9
rbc L-R TGCGGTGAATCCTCCTGTT 58.3
rbc S-F CGCTACTGGACCATGTGGAA 59.1
rbc S-R ACTGCGTCTGCTTGATGTTGT 58.1

图1

外源NO对干旱胁迫下玉米幼苗Rubisco活性(A)及活化酶RCA活性(B)的影响处理CK、SNP、PEG、SNP + PEG分别为0% PEG、100 μmol L-1 SNP、20% PEG和100 μmol L-1 SNP + 20% PEG。"

图2

外源NO对干旱胁迫下玉米rbc S (A)、rbc L (B)和rca β (C)的影响处理CK、SNP、PEG、SNP + PEG分别为0% PEG、100 μmol L-1 SNP、20% PEG和100 μmol L-1 SNP + 20% PEG。"

图3

外源NO处理对干旱胁迫下玉米幼苗O2-(A)和H2O2(B)含量的影响处理CK、SNP、PEG、SNP + PEG分别为0% PEG、100 μmol L-1 SNP、20% PEG和100 μmol L-1 SNP + 20% PEG。"

图4

外源NO处理对干旱胁迫下玉米幼苗SOD活性(A)及SOD同工酶(B)的影响处理CK、SNP、PEG、SNP + PEG分别为0% PEG、100 μmol L-1 SNP、20% PEG和100 μmol L-1 SNP + 20% PEG。"

图5

外源NO处理对干旱胁迫下玉米幼苗POD活性(A)及POD同工酶(B)的影响处理CK、SNP、PEG、SNP + PEG分别为0% PEG、100 μmol L-1 SNP、20% PEG和100 μmol L-1 SNP + 20% PEG。"

图6

外源NO处理对干旱胁迫下玉米幼苗CAT活性(A)及CAT同工酶(B)的影响处理CK、SNP、PEG、SNP + PEG分别为0% PEG、100 μmol L-1 SNP、20% PEG和100 μmol L-1 SNP + 20% PEG。"

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