低温胁迫下聚糖萘合剂对玉米幼苗光合作用和抗氧化酶活性的影响

doi:10.3724/SP.J.1006.2012.00352

摘要/Abstract

摘要： 采用盆栽试验, 以郑单958和丰单3号为材料, 研究了低温胁迫对玉米幼苗光合作用、叶绿素、叶绿素荧光参数和抗氧化酶活性的影响, 以及聚糠萘合剂(PKN)的调控效果。结果表明, 低温胁迫下, 玉米幼苗的光合作用和光系统II光化学最大效率受到抑制; 超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性降低; 过氧化氢、超氧阴离子的产生速率及丙二醛(MDA)含量显著升高。PKN处理提高了低温胁迫下玉米幼苗净光合速率(P_n)、气孔导度(G_s)、光系统II光化学的最大效率(F_v/F_m)、叶绿素含量(Chl a+Chl b)。低温处理7 d, ZDTR和FDTR的P_n、G_s、F_v/F_m、Chl a+Chl b分别比各自的对照提高了88.95%和61.11%、593.33%和1 741.67%、111.50%和145.16%、36.61%和54.03%; PKN处理延缓了SOD、POD、CAT活性的降低, 低温胁迫7 d, PKN处理使郑单958和丰单3的SOD、POD、CAT活性分别比对照高了292.59%和632.98%、295.07%和360.54%、254.55%和265.45%; 同时降低了过氧化氢、超氧阴离子的产生速率及MDA的含量。表明PKN处理有利于提高玉米幼苗的抗冷性。

关键词: 冷害胁迫, 抗氧化酶, 光合作用, 玉米幼苗, 化学调控

Abstract: A pet experiment of maize seedlings was conducted to investigate the effects of low temperature stress and the chemical regulator of PASP-KT-NAA (PKN) on photosynthetic, physiological parameters, chlorophyll contents, and various antioxidant enzymes activities. The results showed that, in maize seedlings under low temperature stress the photosynthesis ability and SOD, POD, CAT activities reduced; the formation speed of hydrogen peroxide and superoxide anion, as well as MDA contents increased. PKN pretreatment increased P_n, G_s, F_v/F_m, and chlorophyll contents. Under low temperature stress for seven days, P_n, G_s, F_v/F_m, chlorophyll contents of ZDTR and FDTR were higher than those of CK, which was increased by 88.95% and 61.11%, 593.33% and 1 741.67%, 111.50% and 145.16%, 36.61% and 54.03%. While the formation speed of hydrogen peroxide and superoxide anion reduced, MDA contents decreased too. Simultaneously, SOD, POD, CAT activities were increased by 292.59% and 632.98%, 295.07% and 360.54%, 254.55% and 265.45%. In conclusion, PKN pretreatment can improve the cold resistance of maize seedlings

Key words: Low temperature stress, Antioxidant enzyme, Photosynthesis, Maize seedlings, PKN regulation

徐田军, 董志强, 兰宏亮, 裴志超, 高娇, 解振兴. 低温胁迫下聚糖萘合剂对玉米幼苗光合作用和抗氧化酶活性的影响[J]. 作物学报, 2012, 38(02): 352-359.

XU Tian-Jun, DONG Zhi-Jiang, LAN Hong-Liang, FEI Zhi-Chao, GAO Jiao, JIE Zhen-Xin. Effects of PASP-KT-NAA on Photosynthesis and Antioxidant Enzyme Activities of Maize Seedlings under Low Temperature Stress[J]. Acta Agron Sin, 2012, 38(02): 352-359.

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链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2012.00352

https://zwxb.chinacrops.org/CN/Y2012/V38/I02/352

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