作物学报 ›› 2019, Vol. 45 ›› Issue (7): 1050-1058.doi: 10.3724/SP.J.1006.2019.82054
莫祎1,2,孙志忠2,丁佳2,余东2,孙学武2,盛夏冰2,谭炎宁2,袁贵龙2,袁定阳1,2,3,*(),段美娟1,*()
MO Yi1,2,SUN Zhi-Zhong2,DING Jia2,YU Dong2,SUN Xue-Wu2,SHENG Xia-Bing2,TAN Yan-Ning2,YUAN Gui-Long2,YUAN Ding-Yang1,2,3,*(),DUAN Mei-Juan1,*()
摘要:
从粳稻日本晴和籼稻R1128杂交衍生的重组自交系群体中获得一个稳定遗传的白条纹叶突变体wsl1 (white stripe leaf 1), 世代为F10。与亲本R1128相比, 突变体wsl1表现出白条纹叶, 同时叶脉呈现白化, 该性状在苗期就出现并持续整个生育期; 突变体的株高、每穗总粒数、剑叶长、生育期显著增加, 而结实率显著下降, 其他农艺性状没有显著变化。分蘖期突变体wsl1的叶绿素a、叶绿素b和胡萝卜素含量较杂交亲本R1128显著下降; 透射电镜观察表明, 与野生型相比, 突变体的叶绿体形状异常, 不规则。遗传分析表明, 该突变性状由1对隐性核基因控制。精细定位后发现, 目标基因WSL1位于第1染色体短臂上标记M1-54与标记M1-70之间, 两者相距89.7 kb。生物信息学分析表明候选区间内共有8个开放阅读框, 暂未发现已报道的叶色相关基因; 其中LOC_Os01g02080编码肽基脯氨酰顺反异构酶, GO (Gene Ontology)分类显示其可能与类囊体形成有关, 后续将通过比较测序、qRT-PCR等分子实验来确定候选基因。
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