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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (02): 191-201.doi: 10.3724/SP.J.1006.2012.00191

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Identification, Cloning, and Potential Application of Genes Related to Somatic Embryogenesis in Plant Tissue Culture

YE Xing-Guo1,SHE Mao-Yun1,2, WANG Ke1,DU Li-Pu1,XU Hui-Jun1   

  1. 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility of Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Genetic and Breeding, Ministry of Agriculture, Beijing 100081, China; 2 Department of Agronomy, Purdue University, West Lafayette, IN 47907, USA
  • Received:2011-10-18 Revised:2011-12-13 Online:2012-02-12 Published:2011-12-13

Abstract: Plant embryogenesis or organogenesis in vitro is a complicated asexual reproductive process consisting of many aspects such as phytohormone perception, dedifferentiation of differentiated cells to acquire organogenic competence, re-entry of quiescent cells into cell cycle, and organization of cell division to form specific organ primordia and meristems. In fact, plant regeneration is the result of multigenic interactions and regulatory controls, which are not only affected by plant hormones and other nutrients in the medium, but also showed a close relationship with the physiological state of explants. The effects of exogenous and endogenous hormones on plant regeneration and the regulation of exogenous hormones on endogenous hormones were reviewed in this paper. Research progresses on five classes of genes related to somatic embryogenesis were collectively described. They are somatic embryogenesis receptor-like kinase, arabinogalactan-proteins, nitrite reductase, auxin binding protein, and antioxidant enzyme. Regeneration associated genes are prospected to be potentially used in plant genetic breeding, whose applications will be involved in the improvement of plant regeneration efficiency and transformation efficiency, also in obtaining transgenic plants with bio-safety. However, main candidate genes related to regeneration might vary in different plants or tissues, or function through different pathways. Therefore, cloning and characterization of some important genes related to somatic embryogenesis or organogenesis should be strengthened in future.

Key words: Plants, Tissue culture, Somatic embryogenesis, Organogenesis, Regeneration-associated genes

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