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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (10): 1760-1765.doi: 10.3724/SP.J.1006.2012.01760

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Homozygosity Analysis of Soybean Varieties in Regional Test of China by Using SSR Markers

GUAN Rong-Xia1,FANG Hong-Liang2,HE Yan-Qin3,CHANG Ru-Zhen1,QIU Li-Juan1,*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Soybean Biology, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Bengbu Medical College, Bengbu 233030, China; 3 National Agriculture Technology Extension Center, Agriculture Ministry, Beijing 100026, China
  • Received:2012-01-06 Revised:2012-06-10 Online:2012-10-12 Published:2012-07-27
  • Contact: 邱丽娟, E-mail: qiu_lijuan@263.net, Tel: 010-82105843

Abstract:

Genomic homozygosity of seeds is very important for protection of breeder’s right and sustainable production of agriculture. Highly informative microsatellite (SSR) markers, widely used in soybean genetic mapping and genetic diversity research, are useful for soybean genomic analysis. Thirty pairs of SSR primers were used to test the DNA of 1 068 soybean varieties (lines) from regional trials of China. The average SSR locus homozygosity was 94.9–97.6% each year. Among the 1 068 varieties (lines) in China regional test screened in five years, the soybean varieties (lines) with homozygosity higher than 85% and 90% accounted for 95% and 91.4%, respectively. Only 42 (3.93%) of the varieties (lines) had homozygosity less than 85%, which were mostly from North spring and Huanghuai summer soybean planting areas. Among the 42 varieties (lines) with homozygosity less than 85% only 11 had the yield 5% more than the controls, 20 had a yield reduction of 0.04% to 13.08%. Compared with those with 100% homozygosity, varieties with lower SSR homozygosity also had lower yield in regional trials. It is suggested that criterion of soybean homozygosity in the national regional test should not be less than 90% so as to insure the protection and sustainable utilization of new soybean varieties (lines) in production.

Key words: Regional test, Soybean [Glycine max (L.) Merr.], SSR loci, Homozygosity

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