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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (10): 1752-1759.doi: 10.3724/SP.J.1006.2012.01752

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Allelic Expression Variation of ahFAD2A and its Relationship with Oleic Acid Accumulation in Peanut

HUANG Bing-Yan1,2,ZHANG Xin-You2,*,MIAO Li-Juan2,GAO Wei2,HAN Suo-Yi2,DONG Wen-Zhao2,TANG Feng-Shou2,LIU Zhi-Yong1,*   

  1. 1 College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China; 2 Industrial Research Institute, Henan Academy of Agricultural Sciences / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry of Agriculture / Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou 450002, China
  • Received:2012-02-20 Revised:2012-06-10 Online:2012-10-12 Published:2012-07-27
  • Contact: 刘志勇, E-mail: zhiyongliu@cau.edu.cn, Tel: 010-62731211; 张新友, E-mail: haasz@sohu.com, Tel: 0371-65729560

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Abstract:

ahFAD2A is a key regulator controlling oleic acid content in peanut. Using ahFAD2A allelic specific primers, the allelic variations of ahFAD2A were tested in 52 peanut varieties and the DNA sequences of 13 typical varieties, including Yuanza 9102, Yuhua 9416, wt08-0932 and wt08-0934, were compared. PCR results revealed that the presence of two alleles of ahFAD2A (referred as to wild type ahFAD2A-wt andmutant ahFAD2A-m respectively) in peanut germplasm, and the G/A single nucleotide polymorphism (SNP) at 448 bp site of ahFAD2A was further confirmed by sequences comparison of 13 peanut varieties. Allelic expression variations of ahFAD2A alleles in peanut seeds were detected by Real-time PCR at different developmental stages. The results indicated that the expression level of mutant allele (ahFAD2A-m) from Yuhua 9416 was slightly higher than that of the wild type allele (ahFAD2A-wt) from Yuanza 9102 during the early to middle developmental stages (17–38 days). However, a rapid decrease in expression level was observed for the mutant allele as compared with its wild type at the late developmental stage (after 45 days). Further determination revealed that the ahFAD2A-m genotype showed higher oleic acid content than linoleic acid content with high O/L ratio (>1.0) starting at early seed developmental stages in Yuhua 9416 while the ahFAD2A-wtgenotype remained lower oleic acid content than linoleic acid content with steady O/L ratio (<1.0) until seed maturity stage in Yuanza 9102. This relationship between oleic acid accumulation and ahFAD2A allelic expression variation in peanut provide the fundamental information for genetic regulation and improvement of seed oleic acids content.

Key words: Arachis hypogaea L., Oleic acid content, Linoleic acid content, ahFAD2A, Allelic variation, Allelic expression variation

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