欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2009, Vol. 35 ›› Issue (2): 262-269.doi: 10.3724/SP.J.1006.2009.00262

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

中国滇蔗茅种质资源遗传多样性的AFLP分析

刘新龙1;蔡青2,*;毕艳1;陆鑫1;马丽1;应雄美1   

  1. 1云南省农业科学院甘蔗研究所，云南开远661600；2云南省农业科学院生物技术与种质资源研究所，云南昆明650223
  • 收稿日期:2008-06-16 修回日期:2008-09-04 出版日期:2009-02-12 网络出版日期:2008-12-11
  • 通讯作者: 蔡青
  • 基金资助:

    本研究由云南省重点基金项目(2006C0013Z)，国家财政部行业专项（nyhyzx07-019），科技部广西省部会商项目（2007BAD30B02）资助

Genetic Diversity Analysis for Germplasm of Erianthus rockii in China

LIU Xin-Long1,CAI Qing2,*,BI Yan1,LU Xin1,MA Li1,YING Xiong-Mei1   

  1. 1Sugarcane Research institute, Yunnan Academy Academy of Agricultural sciences,Kaiyuan 661600,China;2Biotechnology & Geneic Germplasm institute, Yunnan Academy of agricultural sciences, Kunming 650223,China
  • Received:2008-06-16 Revised:2008-09-04 Published:2009-02-12 Published online:2008-12-11
  • Contact: CAI Qing

PDF

1480

被引次数

44

摘要：

利用10对AFLP引物对来自国家甘蔗资源圃的41份滇蔗茅(Erianthus rockii)无性系进行扩增, 获得860个片段, 多态性条带629个, 多态性条带比率0.73, 特异片段54个。遗传相似性系数、UPGMA聚类和主效应分析表明, 在相似系数0.52处做切割线，毛轴野古草、斑茅和滇蔗茅无性系分为3个类群；在相似系数0.715处做切割线时, 又将41份滇蔗茅无性系划分为3个大类群, 云滇07/23独自形成A类群, 鉴于其叶鞘背毛，有待做进一步的分析；B类群3份无性系, 主要来自云南西南部高海拔地区；C类群37份无性系, 其中30份来自云南西南方向的保山、德宏地区, 其他地区7份；在相似系数0.738处做切割时, 将C类群37份无性系划分为4个亚类群, 亚类群的划分反映出明显的地域分布规律, 来自同一地区的无性系多聚为一类；在相似系数0.765处做切割可将C4亚类群划分为4个亚类群(C4-1, C4-2, C4-3, C4-4), 其中C4-3亚类群中云滇07/9/1与云滇99/4分子聚类最为相似, 可作为复份材料保存；C4-3亚类群在相似系数0.773处切割又可以分为3个分支类群, 以上分析反映出同一地区无性系之间具有丰富的遗传变异；主效应分析反映的属间、种间、无性系之间的亲缘关系与分子聚类分析结果一致；由此可见, 丰富的地理生态条件造就了滇蔗茅丰富的遗传多样性和明显的地域性分布规律。

关键词: 滇蔗茅, AFLP, 遗传多样性

Abstract:

Erianthus rockii is the most important wild resource in sugarcane germplasm, which mainly distributes in Yunnan Province. To well utilize the germplasm in breeding program, it is necessary to understand its genetic background. In this research, 41 clones from national nursery of sugarcane germ were amplified with 10 informative AFLP primers. In total, 860 bands were detected, 629 of which were polymorphic, 36 of which were specific bands, and rate of polymorphic bands was 0.73. The results of genetic similarity coefficients, UPGMA cluster, and principal component analysis showed that 41 sugarcane clones were divided into three groups at 0.715 of genetic similarity coefficient:Yundian 07/23 was only grouped into group A and should be further identified due to its back hair on the sheath different from others, group B included three sugarcane clones from high altitude area, group C included 37 sugarcane clones, containing of 30 from southwest of Yunnan, and 7 from other region. Groups C was divided into 4 sub-groups at 0.738 of genetic similarity coefficient, and the cluster relation was obviouslyreflected the geographic distribution of the clones.Sub-group C4 was divided into 4 sub-sub-groups at 0.765 of genetic similarity coefficient. Yundian07/9/1 and Yundian99/4 in sub-sub-group C4-3 were the most similar, indicating that two clones were duplicate. Sub-sub-group C4-3 included three branches according to 0.773 of genetic similarity coefficient. Principal component analysis for all clones indicated same result obtained from genetic similarity coefficients analysis. Therefore, complex geographical ecological environment is important factor to the genetic diversity and geographical distribution of E. rockii clones.

Key words: Erianthus rockii, AFLP marker, Genetic diversity

[1] Editorial Board of the Flora of China (中国植物志编辑部委员会). Flora of China, Vol. 9 (中国植物志·第9卷). Beijing: Sci-ence Press, 2002. pp 48-49(in Chinese)
[2] Chen R-K(陈如凯). Theory and Practice in Modern Sugarcane Breeding (现代甘蔗育种的理论与实践). Beijing: China Agri-culture Press, 2003. pp 2-7(in Chinese)
[3] Wang L-P(王丽萍), Cai Q(蔡青), Lu X(陆鑫), Ma L(马丽), Liu X-L(刘新龙), Li W-F(李文凤), Xia H-M(夏红明). Study of wild species Erianthus rockii germplasm innovation and use. Sugar Crops China (中国糖料), 2008, (2): 8-11(in Chinese with Eng-lish abstract)
[4] Xiao F-H(肖凤回). Comparison between E. arundinaceus and some species under Saccharum and Erianthus on the plant mor-phology and isozyme. Sugarcane (甘蔗), 1994, 1(1): 22-27(in Chinese with English abstract)
[5] Cai Q(蔡青), Fan Y-H(范源洪), Aitken K, Piperidis G, Mcintyre C L, Jackson P. Assessment of the phylogenetic relationships within the “Saccharum complex” using AFLP markers. Acta Agron Sin (作物学报), 2005, 31(5): 551-559(in Chinese with English abstract)
[6] Cai Q, Aitken K, Fan Y H, Piperidis G, Jackson P, Mcintyre C L. A preliminary assessment of the genetic relationship between Erian-thus rockii and the ‘‘Saccharum complex’’ using micro-satellite (SSR) and AFLP markers. Plant Sci, 2005, 169: 976-984
[7] Guo X-M(郭雄明), Xue X(薛霞), Chen H(陈华). Progress in re-search on amplified fragment length polymorphism (AFLP). China J Comparative Med (中国比较医学杂志), 2006, 16(6): 369-372(in Chinese with English abstract)
[8] Doyle J J, Doyle J I. Isolation of plant DNA from fresh tissue. Focus, 1990, 12: 149-151
[9] Vos P, Hogers R, Bleeker M, Reijans M, Van Der Lee T, Hornes M. AFLP: A new concept for DNA fingerprinting. Nucl Acids Res, 1995, 23: 4407-4414
[10] Powell W, Morgante M, Andre C. The comparison RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed, 1996, 2: 225-238
[11] Nei M, Kumar S. Molecular Evolution and Phylogenetics. New York: Oxford University Press, 2000. pp 87-88
[12] Xu A-X(徐爱遐), Ma C-Z(马朝芝), Xiao E-S(肖恩时), Quan J-C(权景春), Ma C-Z(马长珍), Tian G-W(田广文), Tu J-X(涂金星), Fu T-D(傅廷栋), Zhang G-S(张改生). Genetic diversity of Brassica juncea from western China. Acta Agron Sin (作物学报), 2008, 34(5): 754-763(in Chinese with English abstract)
[13] Yan L(闫龙), Guan J-P(关建平), Zong X-X(宗绪晓). Genetic diversity analysis of pigeonpea germplasm resources by AFLP. Acta Agron Sin (作物学报), 2007, 33(5): 790-798(in Chinese with English abstract)
[14] Liu Y(刘勇), Sun Z-H(孙中海), Liu D-C(刘德春), Wu B(吴波), Tao J-J(陶建军). Assessment of the genetic diversity of pummelo germplasms using AFLP and SSR markers. Sci Agric Sin (中国农业科学), 2005, 38(11): 2308-2315(in Chinese with English ab-stract)
[15] Zhuang N-S(庄南生), Zheng C-M(郑成木), Huang D-Y(黄东益), Tang Y-Q(唐燕琼), Gao H-Q(高和琼). AFLP analysis for sugar-cane Germplasms. Acta Agron Sin (作物学报), 2005, 31(4): 444-450(in Chinese with English abstract)
[16] Aitken K S, Li J C, Jackson P, Piperidis G, Mcintyre C L. AFLP analysis of genetic diversity within Saccharum officinarum and comparison with sugarcane cultivars. Aust J Agric Res, 2006, 57: 1167-1184
[17] Selvi A, Nair N V, Noyer J L, Singh N K, Balasundaram N, Bansal K C, Koundal K R, Mohapatra T. AFLP analysis of the phenetic organization and genetic diversity in the sugarcane com-plex, Saccharum and Erianthus. Genet Resour Crop Evol, 2006, 53: 831-842
[18] Liu X-L(刘新龙), Cai Q(蔡青), Wang L-P(王丽萍), Ma L(马丽), Lu X(陆鑫), Ying X-M(应雄美), Mao J(毛钧). The advance of sugarcane germplasm resource in genetic and crossing diversity utilization. Sugar Crops China (中国糖料), 2007, (1): 46-49(in Chinese with English abstract)
[1] 肖颖妮, 于永涛, 谢利华, 祁喜涛, 李春艳, 文天祥, 李高科, 胡建广. 基于SNP标记揭示中国鲜食玉米品种的遗传多样性[J]. 作物学报, 2022, 48(6): 1301-1311.
[2] 王琰琰, 王俊, 刘国祥, 钟秋, 张华述, 骆铮珍, 陈志华, 戴培刚, 佟英, 李媛, 蒋勋, 张兴伟, 杨爱国. 基于SSR标记的雪茄烟种质资源指纹图谱库的构建及遗传多样性分析[J]. 作物学报, 2021, 47(7): 1259-1274.
[3] 刘少荣, 杨扬, 田红丽, 易红梅, 王璐, 康定明, 范亚明, 任洁, 江彬, 葛建镕, 成广雷, 王凤格. 基于农艺及品质性状与SSR标记的青贮玉米品种遗传多样性分析[J]. 作物学报, 2021, 47(12): 2362-2370.
[4] 孙倩, 邹枚伶, 张辰笈, 江思容, Eder Jorge de Oliveira, 张圣奎, 夏志强, 王文泉, 李有志. 基于SNP和InDel标记的巴西木薯遗传多样性与群体遗传结构分析[J]. 作物学报, 2021, 47(1): 42-49.
[5] 赵孟良,王丽慧,任延靖,孙雪梅,侯志强,杨世鹏,李莉,钟启文. 257份菊芋种质资源表型性状的遗传多样性[J]. 作物学报, 2020, 46(5): 712-724.
[6] 张红岩,杨涛,刘荣,晋芳,张力科,于海天,胡锦国,杨峰,王栋,何玉华,宗绪晓. 利用EST-SSR标记评价羽扇豆属(Lupinus L.)遗传多样性[J]. 作物学报, 2020, 46(3): 330-340.
[7] 刘易科,朱展望,陈泠,邹娟,佟汉文,朱光,何伟杰,张宇庆,高春保. 基于SNP标记揭示我国小麦品种(系)的遗传多样性[J]. 作物学报, 2020, 46(02): 307-314.
[8] 叶卫军,陈圣男,杨勇,张丽亚,田东丰,张磊,周斌. 绿豆SSR标记的开发及遗传多样性分析[J]. 作物学报, 2019, 45(8): 1176-1188.
[9] 吴迷,汪念,沈超,黄聪,温天旺,林忠旭. 基于重测序的陆地棉InDel标记开发与评价[J]. 作物学报, 2019, 45(2): 196-203.
[10] 卢媛,艾为大,韩晴,王义发,李宏杨,瞿玉玑,施标,沈雪芳. 糯玉米自交系SSR标记遗传多样性及群体遗传结构分析[J]. 作物学报, 2019, 45(2): 214-224.
[11] 薛延桃,陆平,史梦莎,孙昊月,刘敏轩,王瑞云. 新疆、甘肃黍稷资源的遗传多样性与群体遗传结构研究[J]. 作物学报, 2019, 45(10): 1511-1521.
[12] 刘洪,徐振江,饶得花,鲁清,李少雄,刘海燕,陈小平,梁炫强,洪彦彬. 基于形态学性状和SSR标记的花生品种遗传多样性分析和特异性鉴定[J]. 作物学报, 2019, 45(1): 26-36.
[13] 白冬梅,薛云云,赵姣姣,黄莉,田跃霞,权宝全,姜慧芳. 山西花生地方品种芽期耐寒性鉴定及SSR遗传多样性[J]. 作物学报, 2018, 44(10): 1459-1467.
[14] 魏中艳, 李慧慧, 李骏, YasirA.Gamar, 马岩松, 邱丽娟. 应用SNP精准鉴定大豆种质及构建可扫描身份证[J]. 作物学报, 2018, 44(03): 315-323.
[15] 余斌,杨宏羽,王丽,刘玉汇,白江平,王蒂,张俊莲. 引进马铃薯种质资源在干旱半干旱区的表型性状遗传多样性分析及综合评价[J]. 作物学报, 2018, 44(01): 63-74.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2