玉米自交系B73全基因组NBS类型抗病基因分析

doi:10.3724/SP.J.1006.2009.00566

摘要/Abstract

摘要：

核苷酸结合位点(NBS)类型抗病基因是植物抗病基因中最大的一个类别，也是近年来植物抗病分子育种研究的一大热点。本研究对玉米自交系B73全基因组中含有NBS结构的候选抗病基因进行了基因总数、类型、系统进化关系等分析。在B73全基因组中含有165个NBS结构的基因，远远少于水稻中的同类基因，按照N-端结构和亮氨酸富集区(LRR)结构, 将其分为153个标准结构和12个非标准结构NBS基因。其中，标准结构基因又分为CC-NBS-LRR、CC-NBS、NBS、NBS-NBS、 NBS-LRR、NBS-NBS-LRR、NBS-X、X-NBS等8个类型。系统进化树分析表明，NBS类型抗病基因存在明显的两大分支，与水稻的星状发散型分布有很大差异。通过基因家族分析，还发现了玉米NBS类型基因的复制现象，但发生复制的基因比例低于水稻，可能是造成玉米NBS抗病基因数目较少的原因之一。

关键词: 玉米, 生物信息学, 抗病基因, 核苷酸结合位点(NBS), 系统进化树

Abstract:

Nucleotide-binding site (NBS) disease resistance gene is a largest category in plant disease resistance genes, which is a focus in recent studies on molecular breeding of plant disease resistance. Using maize (Zea mays L.) inbred line B73, the complete set of disease resistance candidate genes that encode NBS was identified in the genome. The putative NBS genes were characterized with respect to structural diversity, phylogenetic relationships and so on. One hundred and sixty-five NBS-coding sequences were identified into two types: nonregular (12) and regular NBS genes (153). The amount of NBS genesis much smaller in maize than in japonica rice (Oryza sativa L.). The 153 regular NBS genes were categorized into eight classes, including CC-NBS-LRR, CC-NBS, NBS, NBS-NBS, NBS-LRR, NBS-NBS-LRR, NBS-X, and X-NBS, according to N-terminal motif and leucine-rich repeat (LRR) domains motif. The 165 NBS genes showed two remarkable branches in the phylogenetic tree, differing from the radiation structure in japonica rice. Gene duplication event was observed based on gene family analysis of the NBS disease-resistance genes in maize; however, the ratio of gene duplication was smaller than that in rice. This might be one of the reasons for less NBS disease-resistance genes in maize than in rice.

Key words: Zea mays L., Bioinformatics, Disease resistance gene, Nucleotide binding sity, Phylogenetic tree

汪结明;江海洋;赵阳;项艳;朱苏文;程备久. 玉米自交系B73全基因组NBS类型抗病基因分析[J]. 作物学报, 2009, 35(3): 566-570.

WANG Jie-Ming;JIANG Hai-Yang;ZHAO Yang;XIANG Yan;ZHU Su-Wen;CHENG Bei-Jiu. Genome-Wide Analysis of NBS-Encoding disease Resistance Genes in Maize Inbred Line B73[J]. Acta Agron Sin, 2009, 35(3): 566-570.

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链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2009.00566

https://zwxb.chinacrops.org/CN/Y2009/V35/I3/566

参考文献

[1]Duan M-X(段民孝), Zhao J-R(赵久然), Wang Y-D(王元东). Study progress in starch of corn Kerne. J Maize Sci (玉米科学), 2002, 10(1): 29–32 (in Chinese with English abstract)
[2]Richly E, Kurth J, Leister D. Mode of amplification and reorganization of resistance genes during recent Arabidopsis thaliana evolution. Mol Biol Evol, 2002, 19: 76–84
[3]Staskawicz B J, Ausubel F M, Baker B J, Ellis J G, Jones J D. Molecular genetics of plant disease resistance. Science, 1995, 268: 661–667
[4]Holub E. Arms race is an ancient history in Arabidopsis, the wildflower. Nat Rev Genet, 2001, 2: 516–527
[5]Meyers B C, Kaushik S, Nandety R S. Evolving disease resistance genes. Curr Opin Plant Biol, 2005, 8: 129–134
[6]Dangl L J, McDowell J M. Two modes of pathogen recognition by plants. Proc Natl Acad Sci USA, 2006, 103: 8575–8576
[7]Noutoshi Y, Ito T, Seki M, Nakashita H, Yoshida S, Marco Y, Shirasu K, Shinozaki K. A single amino acid insertion in the WRKY domain of the Arabidopsis TIR-NBS-LRR-WRKY-type disease resistance protein SLH1 (sensitive to low humidity 1) causes activation of defense responses and hypersensitive cell death. Plant J, 2005, 43: 873–888
[8]Montesinos E, Bonaterra A, Badosa E, Francés J, Alemany J, Llorente I, Moragrega C. Plant-microbe interactions and the new biotechnological methods of plant disease control. Int Microbiol, 2002, 5: 169–175
[9]Lupas A, Van Dyke M, Stock J. Predicting coled coils from protein sequences. Science, 1991, 252: 1162–1164
[10]Xu J H, Messing J.Organization of the prolamin gene family provides insight into the evolution of the maize genome and gene duplications in grass species. Proc Natl Acad Sci USA, 2008, 23:105–138
[11]Tian D, Traw M B, Chen J Q, Kreitman M, Bergelson J. Fitness cost of R-gene mediated resistance in Arabidopsis thaliana. Nature, 2003, 423: 74–77
[12]Chen G, Pan D, Zhou Y, Lin S, Ke X. Diversity and evolutionary relationship of nucleotide binding site-encoding disease-resistance gene analogues in sweet potato (Ipomoea batatas Lam.). J Biosci, 2007, 32: 713–721
[13]Xu Q, Wen X, Deng X. Isolation of TIR and non-TIR NBS-LRR resistance gene analogues and identification of molecular markers linked to a powdery mildew resistance locus in chestnut rose (Rosa roxburghii Tratt). Theor Appl Genet, 2005, 111: 819–830
[14]Plocik A, Layden J, Kesseli R. Comparative analysis of NBS domain sequences of NBS-LRR disease resistance genes from sunflower, lettuce, and chicory. Mol Phylogenet Evol, 2004, 31: 153–163
[15]Zhou T, Wang Y, Chen J Q, Araki H, Jing Z, Jiang K, Shen J, Tian D C. Genome-wide identification of NBS genes in japonica rice reveals significant expansion of divergent non-TIR NBS-LRR genes. Mol Genet Genomics, 2004, 271: 402–415
[16]Bai J, Pennill L A, Ning J, Lee S W, Ramalingam J, Webb C A, Zhao B, Sun Q, Nelson J C, Leach J E, Hulbert S H. Diversity in nucleotide binding site-leucine-rich repeat genes in cereals. Genome Res, 2002, 12: 1871–1884
[17]Peng G-Z(彭贵子), Chen L-L(陈玲玲), Tian D-C(田大成). Progress in the study of gene duplication. Hereditas (遗传), 2006, 28(7): 886–892 (in Chinese with English abstract)
[18]Meyers B C, Morgante M, Michelmore R W. TIR-X and TIR-NBS proteins: Two new families related to disease resistance TIR-NBS-LRR proteins encoded in Arabidopsis and other plant genomes. Plant J, 2002, 32: 77–92
[19]Meyers B C, Kozik A, Griego A, Kuang H, Michelmore R W. Genome-wide analysis of NBS-LRR-Encoding genes in Arabidopsis. Plant Cell, 2003, 15: 809–834
[20]Noir S, Combes M C, Anthony F, Lashermes P. Origin, diversity and evolution of NBS-type disease-resistance gene homologues in coffee trees (Coffea L.). Mol Genet Genomic, 2001, 265: 654–662
[21]López C E, Zuluaga A P, Cooke R, Delseny M, Tohme J, Verdier V. Isolation of resistance gene candidates (RGCs) and characterization of an RGC cluster in cassava. Mol Genet Genomic, 2003, 269: 658–671
[22]Tan X, Meyers B C, Kozik A, West M A, Morgante M, St Clair D A, Bent A F, Michelmore R W. Global expression analysis of nucleotide binding site-leucine rich repeat-encoding and related genes in Arabidopsis. BMC Plant Biol, 2007, 23: 56–76
[23]Mondragón-Palomino M, Meyers B C, Michelmore R W, Gaut B S. Patterns of positive selection in the complete NBS-LRR gene family of Arabidopsis thaliana. Genome Res, 2002, 12: 1305–1315
[24]Yaish M W, Sáenz de Miera L E, Pérez de la Vega M. Isolation of a family of resistance gene analogue sequences of the nucleotide binding site (NBS) type from Lens species. Genome, 2004, 47: 650–659
[25]Yang S H, Zhang X H, Yue J X, Tian D C, Chen J Q. Recent duplications dominate NBS-encoding gene expansion in two woody species. Mol Genet Genomics, 2008, 280: 187–198
[26]Kohler A, Rinaldi C, Duplessis S, Baucher M, Geelen D, Duchaussoy F, Meyers BC, Boerjan W, Martin F. Genome-wide identification of NBS resistance genes in Populus trichocarpa. Plant Mol Biol, 2008, 66: 619–636
[27]Pan Q, Wendel J, Fluhr R. Divergent evolution of plant NBS-LRR resistance gene homologues in dicot and cereal genomes. J Mol Evol, 2000, 50: 203–213
[28]Shirano Y, Kachroo P, Shah J, Klessig D F. A gain-of-function mutation in an Arabidopsis Toll Interleukin receptor-nucleotide binding site-leucine-rich repeat type R gene triggers defense responses and results in enhanced disease resistance. Plant Cell, 2002, 14: 3149–3162

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