欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2009, Vol. 35 ›› Issue (9): 1597-1605.doi: 10.3724/SP.J.1006.2009.01597

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用cDNA-AFLP技术分析小麦应答低磷胁迫的特异表达基因

谷俊涛2,鲍金香1,王效颖1,郭程瑾1,李小娟2,路文静2,肖凯1,*   

  1. 1河北农业大学农学院，河北保定071001；2河北农业大学生命科学学院，河北保定071001
  • 收稿日期:2009-01-07 修回日期:2009-03-15 出版日期:2009-09-12 网络出版日期:2009-07-03
  • 通讯作者: 肖凯, E-mail: xiaokai@hebau.edu.cn
  • 基金资助:

    本研究由国家重点基础研究发展计划（973计划）前期项目(2007CB116209)，河北省重点基础研究项目(08965525D)，河北省自然科学基金(C2007000476)资助。

Investigation Based on cdna-aflp Approach for Differential Expressed Genesis Responding to Deficient-Pi in Wheat

GU Jun-Tao2,BAO Jin-Xiang1,WANG Xiao-Ying1,GUO Cheng-Jin1,LI Xiao-Juan2,LU Wen-Jing2,XIAO Kai1*   

  1. 1 College of Agronomy, Agricultural University of Hebei, Baoding 071001, China, 2College of Life Science; Agricultural University of Hebei, Baoding 071001, China
  • Received:2009-01-07 Revised:2009-03-15 Published:2009-09-12 Published online:2009-07-03
  • Contact: XIAO Kai, E-mail: xiaokai@hebau.edu.cn

PDF

4144

被引次数

35

摘要：

以磷高效小麦品种石新828为材料，采用cDNA-AFLP技术，鉴定了短期(1~6 h)、中期(12~48 h)和长期(72~144 h)低磷胁迫根系特异上、下调表达基因的表达序列标签(EST)。共有非重复的上调ESTs 142个，下调ESTs 94个。胁迫下的前者分别含短、中和长期23、53和66个；后者分别含短、中和长期17、39和38个。对其功能比对发现，上调ESTs在功能上归属于信号转导、转录调控、代谢、逆境响应、发育、物质运输、脂类代谢和功能未知等类别，下调EST除上述类别外，还含有蛋白质合成和降解等类别。部分转录因子基因(如水稻OsPTF1和拟南芥ZAT10高度同源的转录因子基因)、促分裂原激酶基因MAPK1a、钙依赖蛋白激酶基因CPK1A和蛋白激酶基因(如serine/threonine kinase)、高亲和磷转运蛋白基因(PHT3和PT2)、过氧化物酶基因(如peroxidase 73)和谷胱甘肽-S-转移酶基因(glutathione S-transferase)，受到低磷胁迫的特异增强诱导，在改善小麦植株对低磷胁迫的适应能力中可能具有重要作用。研究表明，小麦对低磷胁迫的响应，在分子水平上存在着植株感受低磷胁迫信号和信号转导、进一步在生理生化方面对胁迫信号产生应答等复杂的过程。

关键词: 小麦, 低磷胁迫, cDNA-AFLP, 特异表达基因, 基因功能类别

Abstract:

To date, differential expression genes in response to deficient-Pi stress have been identified and well studied in Arabidopsis thaliana, and totally 612 up-regulated and 254 down-regulated genes with various functions were reported. However, there is no similar report in wheat (Triticum aestivum L.). In this study, seedlings of wheat cultivar Shixin 828 with high phosphorus use efficiency were treated with 20 µmol L-1 Pi for 1 to 144 h, and the differential expressed sequence tags (ESTs) with up-regulated and down-regulated patterns were investigated based on cDNA-AFLP approach after deficient-Pi treatment for short term (1–6 h), medium term (12–48 h), and long term (72–144 h). A total of 142 nonredundant ESTs with up-regulated pattern were identified, in which 23, 53, and 66 ESTs expressed in treatments of short, medium, and long term, respectively. Simultaneously, 94 nonredundant ESTs with down-regulated pattern were detected in treatments of short (17), medium (39), and long term (38). These ESTs were classified into several functional groups with BLAST in GenBank. Except for 44 function-unknown ESTs with the up-regulated pattern, the remained up-regulated ESTs conferred functions of signal transduction, transcription regulation, metabolism, stress response, development, transport, and lipid metabolism. Besides the above functions, protein synthesis and protein degradation were also observed in the down-regulated ESTs. Some genes of transcription factors (such as the transcription factor genes with high homologous to rice OsPTF1 and Arabidospsis ZAT10), mitogen activated protein kinase (MAPK1a), calcium-dependent protein kinase (CPK1A), and protein kinase (such as serine/threonine kinase), high-affinity phosphate transporter (PHT3 and PT2), peroxidase (such as peroxidase 73) and glutathione (glutathione S-transferase) were specifically up-regulated under deficient-Pi condition. This suggested that they might play important roles in promoting adaptation to deficient-Pi environment.

Key words: Wheat(Triticum aestivum L.), Low-Pi stress, cDNA-AFLP approach, Expressed sequence tags(ESTs), Functional classification of genes

[1] 胡文静, 李东升, 裔新, 张春梅, 张勇. 小麦穗部性状和株高的QTL定位及育种标记开发和验证[J]. 作物学报, 2022, 48(6): 1346-1356.
[2] 郭星宇, 刘朋召, 王瑞, 王小利, 李军. 旱地冬小麦产量、氮肥利用率及土壤氮素平衡对降水年型与施氮量的响应[J]. 作物学报, 2022, 48(5): 1262-1272.
[3] 付美玉, 熊宏春, 周春云, 郭会君, 谢永盾, 赵林姝, 古佳玉, 赵世荣, 丁玉萍, 徐延浩, 刘录祥. 小麦矮秆突变体je0098的遗传分析与其矮秆基因定位[J]. 作物学报, 2022, 48(3): 580-589.
[4] 冯健超, 许倍铭, 江薛丽, 胡海洲, 马英, 王晨阳, 王永华, 马冬云. 小麦籽粒不同层次酚类物质与抗氧化活性差异及氮肥调控效应[J]. 作物学报, 2022, 48(3): 704-715.
[5] 刘运景, 郑飞娜, 张秀, 初金鹏, 于海涛, 代兴龙, 贺明荣. 宽幅播种对强筋小麦籽粒产量、品质和氮素吸收利用的影响[J]. 作物学报, 2022, 48(3): 716-725.
[6] 马红勃, 刘东涛, 冯国华, 王静, 朱雪成, 张会云, 刘静, 刘立伟, 易媛. 黄淮麦区Fhb1基因的育种应用[J]. 作物学报, 2022, 48(3): 747-758.
[7] 徐龙龙, 殷文, 胡发龙, 范虹, 樊志龙, 赵财, 于爱忠, 柴强. 水氮减量对地膜玉米免耕轮作小麦主要光合生理参数的影响[J]. 作物学报, 2022, 48(2): 437-447.
[8] 王洋洋, 贺利, 任德超, 段剑钊, 胡新, 刘万代, 郭天财, 王永华, 冯伟. 基于主成分-聚类分析的不同水分冬小麦晚霜冻害评价[J]. 作物学报, 2022, 48(2): 448-462.
[9] 陈新宜, 宋宇航, 张孟寒, 李小艳, 李华, 汪月霞, 齐学礼. 干旱对不同品种小麦幼苗的生理生化胁迫以及外源5-氨基乙酰丙酸的缓解作用[J]. 作物学报, 2022, 48(2): 478-487.
[10] 马博闻, 李庆, 蔡剑, 周琴, 黄梅, 戴廷波, 王笑, 姜东. 花前渍水锻炼调控花后小麦耐渍性的生理机制研究[J]. 作物学报, 2022, 48(1): 151-164.
[11] 孟颖, 邢蕾蕾, 曹晓红, 郭光艳, 柴建芳, 秘彩莉. 小麦Ta4CL1基因的克隆及其在促进转基因拟南芥生长和木质素沉积中的功能[J]. 作物学报, 2022, 48(1): 63-75.
[12] 韦一昊, 于美琴, 张晓娇, 王露露, 张志勇, 马新明, 李会强, 王小纯. 小麦谷氨酰胺合成酶基因可变剪接分析[J]. 作物学报, 2022, 48(1): 40-47.
[13] 李玲红, 张哲, 陈永明, 尤明山, 倪中福, 邢界文. 普通小麦颖壳蜡质缺失突变体glossy1的转录组分析[J]. 作物学报, 2022, 48(1): 48-62.
[14] 罗江陶, 郑建敏, 蒲宗君, 范超兰, 刘登才, 郝明. 四倍体小麦与六倍体小麦杂种的染色体遗传特性[J]. 作物学报, 2021, 47(8): 1427-1436.
[15] 王艳朋, 凌磊, 张文睿, 王丹, 郭长虹. 小麦B-box基因家族全基因组鉴定与表达分析[J]. 作物学报, 2021, 47(8): 1437-1449.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2