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作物学报 ›› 2010, Vol. 36 ›› Issue (07): 1209-1215.doi: 10.3724/SP.J.1006.2010.01209

• 研究简报 • 上一篇    下一篇

基因型和环境条件对小麦花药培养效果的影响

韩晓峰1,2,陶丽莉2,殷桂香2,刘晓蕾2,3,杜丽璞2,魏亦勤4,晏月明1,*,叶兴国2,*   

  1. 1 首都师范大学生命科学学院，北京 100048；2 中国农业科学院作物科学研究所，北京 100081；3 北方民族大学生命科学与生物工程学院，宁夏银川 750021；4宁夏农林科学院农作物科学研究所，宁夏永宁 750105
  • 收稿日期:2010-01-04 修回日期:2010-03-05 出版日期:2010-07-12 网络出版日期:2010-04-28
  • 通讯作者: 晏月明, E-mail: yanym2004@163.com, Tel: 010-68902777; 叶兴国, E-mail: yexg@mail.caas.net.cn, Tel: 010-82109765
  • 基金资助:

    本研究由国家自然科学基金项目(30971776)和宁夏农业厅重点合作项目资助.

Effect of Genotype and Growing Environment on Anther Culture in Wheat

HAN Xiao-Feng1,2,DAO Li-Li2,YIN Gui-Xiang2,LIU Xiao-Lei2,3,DU Li-Pu2,WEI Yi-Qin4,YAN Yue-Ming1,*,YE Xing-Guo2,*   

  1. 1 College of Life Sciences, Capital Normal University, Beijing 100048, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081；3 College of Life Sciences and Biology Engineering, North University for Ethnics, Yinchuan 750021, China；4 Crop Research Institute, Ningxia Academy of Agricultural and Forestry Sciences, Yongning 750105, China
  • Received:2010-01-04 Revised:2010-03-05 Published:2010-07-12 Published online:2010-04-28
  • Contact: YAN Yue-Ming,E-mail:yanym2004@163.com, Tel: 010-68902777;YE Xing-Guo,E-mail: yexg@mail.caas.net.cn, Tel: 010-82109765

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1638

被引次数

36

摘要：

为进一步提高小麦花培育种效率，明确花药培养力的遗传控制基础，以11个小麦品种及其组配的20个F1杂种为材料，探讨了基因型、培养基和环境条件对愈伤组织诱导率的影响。在W14D、W14gD、W14GD培养基上，Alondra、Verry、石4185、新春9号和百农3217的花药易被诱导产生愈伤组织，诱导率为25.3%~51.9%，其中石4185是目前公认的花培育种优良亲本，新春9号为新发现的优良花培基因型。以宁春4号配制的部分F1杂种的愈伤组织诱导率较高，大多数组合高于10.0%，表明宁春4号与供试品种间具有较高的花药培养配合力。小麦花培育种技术要求亲本之一具有较高的花药愈伤组织诱导率或较高的花药培养配合力。小麦花药培养力的遗传控制复杂，表现为数量性状遗传，亲本花药培养力很高，其F1组合花药培养力不一定很高，这与双亲配合力有关。小麦花药培养中，供体植株生长和愈伤组织诱导的适宜条件为较长的营养生长期、适宜的前期(分蘖期)温度和较高的中期(拔节后期)温度。在添加低浓度生长素和葡萄糖的液体培养基中发现小麦花药直接成苗现象，2,4-D诱导花药直接成苗效果优于Dicamba。随着年度间气候升高的影响，相同基因型花药愈伤组织诱导率呈现增加趋势。

关键词: 小麦, 花药培养, 基因型, 环境条件, 愈伤组织诱导, 花药直接成苗

Abstract:

Wheat anther culture is affected greatly by many factors, such as wheat genotype, culture medium, and the temperature during the growing period of donors. For further improving the regeneration efficiency from wheat anthers and understanding the genetic control of wheat anther culture ability, some wheat genotypes including Verry, Xinchun 9, Chinese Spring, and Ningchun 4, and their F1 hybrids by crossing each other were used for anther cultures in different years, and the callus induction frequencies were evaluated. The results indicated that haploid calli were easily induced from the anthers of Alondra, Verry, Shi 4185, Xinchun 9, and Bainong 3217. The frequency of callus production ranged from 25.3% to 51.9%. Shi 4185 and Xinchun 9 showed higher potentials for wheat haploid breeding because of their good agronomic traits and high anther culture response. Some F1environment conditions for the donor plants of the anthers were long vegetative growing period, moderate temperature during the tillering period, and high temperature during the hybrids derived from Ningchun 4 had high callus production rates, indicating that Ningchun 4 had high combining ability with some crossing parents in anther culture. However, the F1 hybrids from the parents with high anther culture response were not certain to perform high callus frequency, which was more than 10.0% in majority of the crosses. This suggested that the genetic control of wheat anther culture appeared to be complicated and performed as a quantitative trait. The optimal late jointingperiod. Haploid plantlets could be induced directly from wheat anthers in the liquid media with low concentrations of auxin and glucose. It was also found that 2,4-D was better than dicamba in liquid media for callus induction. Besides, the frequency of callus induced from wheat anthers showed increase trend with the promotion of temperature during the growing period of wheat donor plants, which was caused by climate warming.

Key words: Wheat, Anther culture, Genotype, Environment conditions, Callus induction, Plantlets induction directly from anthers


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