玉米Rubisco活化酶基因ZmRCA1的序列变异分析

doi:10.3724/SP.J.1006.2011.00058

摘要/Abstract

摘要： Rubisco活化酶(RCA)是一种可激活光合作用关键酶Rubisco的伴侣蛋白，可通过对Rubisco的活性调节决定植物的碳同化效率。为了研究玉米ZmRCA1的多态性，本研究参考GenBank中编码玉米Rubisco活化酶的ZmRCA1基因组序列设计引物对玉米微核心种质的95份自交系的ZmRCA1进行测序，获得长约1 680 bp的基因组序列。多态分析表明，在1 680 bp的区间内共发现22个SNP和8个InDel，其中5个SNP和1个InDel变异产生氨基酸序列改变；频率在0.1以上的13个多态性位点共形成27种单倍型，利用6个多态性位点就可以分辨约90%的单倍型。ZmRCA1基因具有高度序列保守性，基因DNA序列相似性为97.9%,而氨基酸序列的相似性则达99.8%；中性检验表明ZmRCA1基因符合中性进化模型假设，没有发生纯化选择。

关键词: Rubisco活化酶(RCA), 单核苷酸多态性(SNP), InDel, 连锁不平衡(LD), 单倍型, 单倍型标签SNP

Abstract: Rubisco is a pivotal enzyme that initiates the first step of carbon fixation and photorespiration in plant photosynthesis. Rubisco accounts for up to half of the soluble protein in the leaves of plants. Nevertheless the catalytic rate of Rubisco iscomparatively low. Rubisco activity is regulated mainly by Rubisco activase, which serves as a molecular chaperone. By activating and regulating Rubisco, RCA potentially influences the efficiency of carbon assimilation in plants. Thereby, RCA has identified a possible target gene for improve production in crops breeding.To investigate polymorphism of ZmRCA1, sequened and analyzed the genomic sequences of ZmRCA1 from a minicore set of 95 maize inbreds lines. Totally 22 SNPs and 8 InDels were identified in a 1 680 bp sequence alignment. There were five SNPs and one InDel which generated amino acid sequence variation. A total of 27 haplotypes were identified with 13 polymorphic loci which the frequency was 0.1 or more. Approximately 90% haplotypes could be distinctly distinguished by six polymorphic loci. The ZmRCA1 gene was highly conserved, with the genic similarity of 97.9% and the amino acid sequence similarity of 99.8%. Neutrality tests showed that no purifying selection occurred in ZmRCA1.

Key words: Rubisco activase (RCA), SNP, InDel, LD, Haplotype, htSNP

谭贤杰, 宋燕春, 石云素, 程伟东, 吴子恺, 王天宇, 黎裕. 玉米Rubisco活化酶基因ZmRCA1的序列变异分析[J]. 作物学报, 2011, 37(01): 58-66.

TAN Xian-Jie, SONG Yan-Chun, SHI Yun-Su, CHENG Wei-Dong, WU Zi-Kai, WANG Tian-Yu, LI Yu. Analysis of Sequence Polymorphism of ZmRCA1 in Maize[J]. Acta Agron Sin, 2011, 37(01): 58-66.

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