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作物学报 ›› 2016, Vol. 42 ›› Issue (06): 932-941.doi: 10.3724/SP.J.1006.2016.00932

• 研究简报 • 上一篇    

籼粳稻基因组295个InDel标记的开发

初志战1,郭海滨2,曾栋昌1,刘耀光1,*   

  1. 1华南农业大学生命科学学院 / 亚热带农业生物资源保护与利用重点实验室,广东广州 510642;2华南农业大学公共基础课实验教学中心, 广东广州 510642
  • 收稿日期:2015-11-09 修回日期:2016-01-11 出版日期:2016-06-12 网络出版日期:2016-03-14
  • 通讯作者: 刘耀光, E-mail: ygliu@scau.edu.cn
  • 基金资助:

    本研究由亚热带农业生物资源保护与利用国家重点实验室开放课题(SKL-CUSAb-2013-04)和广东省自然科学基金-博士启动项目(2015A030310485)资助。

Development of 295 InDel Markers for Indica andJapanicaRice

CHU Zhi-Zhan1,GUO Hai-Bin2,ZENG Dong-Chang1,LIU Yao-Guang1,*?   

  1. 1 College of Life Sciences, South China Agricultural University/ State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangzhou 510642, China;2Center of Experimental Teaching for Common Basic Course, South China Agricultural University,Guangzhou 510642,China
  • Received:2015-11-09 Revised:2016-01-11 Published:2016-06-12 Published online:2016-03-14
  • Contact: 刘耀光, E-mail: ygliu@scau.edu.cn
  • Supported by:

    This study was supported by the Open Fund Project of State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources(SKL-CUSAb-2013-04)and the Natural Science Fund of Guangdong Province-the Launch Program for Doctor (2015A030310485).

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摘要：

插入/缺失(InDel)分子标记具有使用简单，结果清晰可靠的优点。本研究通过比对粳稻品种日本晴和籼稻品种93-11的基因组序列，在全基因组范围内设计了634对InDel候选标记，通过PCR检测比较2种粳稻(日本晴和台中65)和2种籼稻(93-11和黄华占)的多态性，发现295对标记在2种籼稻间及2种粳稻间均带型一致，而在籼、粳亚种间有多态性，因此这套295对标记可以在涉及籼粳亚种的基因定位和分子育种中应用。

关键词: InDel标记, 水稻, 基因定位

Abstract:

Insertion/Deletion (InDel) markers have advantages of simplicity and reliability for genotyping.We selected 634 candidate InDelmarkers distributing throughout the 12 chromosomes of riceby comparing genome sequences between the japonica cultivarNipponbare and the indica cultivar 93-11. PCR results of these candidate markers betweentwojaponica(Nipponbare and Taizhong 65) and twoindica（9311 and Huanghuazhan）cultivars revealed that295 InDelmarkersdisplayed common polymorphisms between the japonica and indicacultivars which is useful for gene mapping and molecular breeding involving inindica andjaponicasubspecies.

Key words: InDel marker, Rice, Gene mapping

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