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作物学报 ›› 2012, Vol. 38 ›› Issue (01): 168-173.doi: 10.3724/SP.J.1006.2012.00168

• 研究简报 • 上一篇    下一篇

水稻叶尖早衰突变体lad的形态、生理分析与基因定位

杜青,方立魁,桑贤春,凌英华,李云峰,杨正林,何光华,赵芳明*   

  1. 西南大学水稻研究所 / 转基因植物与安全控制重庆市市级重点实验室, 重庆400716
  • 收稿日期:2011-05-19 修回日期:2011-09-18 出版日期:2012-01-12 网络出版日期:2011-11-07
  • 通讯作者: 赵芳明, E-mail: zhaofangming2004@163.com
  • 基金资助:

    本研究由国家自然科学基金项目(31071072)和重庆市重大攻关项目(CSTC, 2010AA1013)资助。

Analysis of Phenotype and Physiology together with Mapping of a Leaf Apex Dead Gene (lad) in Rice (Oryza sativa L.)

DU Qing,FANG Li-Kui,SANG Xian-Chun,LING Ying-Hua,LI Yun-Feng,YANG Zheng-Lin,HE Guang-Hua,ZHAO Fang-Ming*   

  1. Rice Research Institute, Southwest University / Chongqing Key Laboratory of Application and Safety of Genetically Modified Crops, Chongqing 400716, China?
  • Received:2011-05-19 Revised:2011-09-18 Published:2012-01-12 Published online:2011-11-07
  • Contact: 赵芳明, E-mail: zhaofangming2004@163.com

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1182

被引次数

17

摘要： 叶片作为植物的主要光合作用场所，研究其早衰机制对提高作物的经济产量具有非常重要的意义。本研究报道了一个来自EMS诱变优良恢复系缙恢10号的新水稻叶尖早衰突变体lad (leaf apex dead)，其叶尖在第5片叶抽出前呈正常状态，当第5叶完全抽出之后前5叶的叶尖变黄并最终枯死；随后的叶子在完全抽出后，叶尖也逐渐变黄并枯死。对该突变体的生理生化分析发现，其叶绿素含量、可溶性蛋白含量明显下降，SOD酶活性异常。其株高、叶长、粒数等也都显著降低。经遗传分析，该突变性状受一对隐性单基因控制，利用分子标记将该基因定位于第11染色体SSR标记SWU11-19和SWU11-5之间，遗传距离为13 cM，并且与SSR标记SWU11-25和SWU11-27共分离。本研究结果为该基因的进一步克隆和功能研究奠定了良好基础。

关键词: 水稻, 叶尖早衰死亡, 基因定位, 表型分析, 生理分析

Abstract: Leaf plays an important role in photosynthesis. Studying the leaf premature senescence is important for increasing the economic yield of crop. A new mutant of rice leaf apex dead reported in the paper derived from a restorer line Jinhui10 treated by EMS. In the mutant, the leaf apexes appeared normal before the 5th leaf unfolded. However, the leaf apexes of all leaves except the 5th leaf exhibited yellow and gradually died when the 5th leaf fully expanded; and afterwards the apexes of new fully expanded leaves became yellow and dead. The physiological and biochemical analysis showed that the lad mutant contained lower contents of chlorophyll and soluble protein compared with the wild type control, and the activity of SOD was abnormal. Again, the plant height, leaf length, grain number and seed setting rate of the lad mutant were significantly lower than those of the control. Thepremature senescence mutant was controlled by a single recessive nuclear gene. And the LAD gene was mapped between the SSR markers SWU11-19 and SWU11-5 on the 11th chromosome, with 13 cM of the genetic distance. Furthermore, the LAD gene was co-segregated with the markers SWU11-25 and SWU11-27. The results laid a favorable foundation for further map-based cloning and functional analysis of the LAD gene.

Key words: Rice, Leaf apex dead, Gene mapping, Phenotypic analysis, Physiologic analysis

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