绿豆基因组SSR引物在豇豆属作物中的通用性

doi:10.3724/SP.J.1006.2012.00223

摘要/Abstract

摘要： 分子标记的种间通用性可降低其开发成本，提高利用效率，也有助于促进遗传研究较薄弱物种的分子遗传学研究。本文选取绿豆、小豆、豇豆及饭豆材料各3份, 分析1 205对新开发的绿豆基因组SSR引物在这些材料中的扩增效果，结果显示绿豆基因组SSR引物在豇豆、小豆和饭豆中的通用性比率分别为50.0%、73.3%和81.6%；多态性比率分别为4.1%、1.7%和1.5%；在4个种间均通用的引物469对。这些通用性SSR引物将有助于这4种食用豆类在多样性评价、连锁图谱的构建、基因定位及比较基因组学等方面的研究。

关键词: 豇豆属, SSR, 通用性

Abstract: Cowpea (Vigna unguiculata L. Walp.), mungbean (V. radiata L. Wilczek), rice bean [V. umbellate (Thunb.) Ohwi & Ohashi] and adzuki bean (V. angularis Willd. Ohwi and Ohashi) are major food legumes in China. At present, SSR markers for genetic analysis of these legumes are much limited. Transferability analysis of primers has the vital significance to reduce their development cost and improve their development efficiency. In this study, 1 205 SSRs primers were tested for their transferability and polymorphism by PCR amplification with the genomic DNA of four Vigna species, cowpea, adzuki bean, mungbean and rice bean. The results indicated that the transferability rate of mungbean genomic-SSR in cowpea, adzuki bean and rice bean was 50.0%, 73.3%, and 81.6%, and the ratio of polymorphism SSR primers in these crops was 4.1%, 1.7%, and 1.5%, whereas 32.0% in mungbean. A total of 469 mungbean genomic-SSR primers were detected to be highly transferable among different species of Vigna. The transferability of mungbean genomic-SSR was higher in adzuki bean and rice bean than in cowpea. These transferable markers are useful for further genetic and breeding studies in these species.

Key words: Genus Vigna, SSR, Transferability

钟敏, 程须珍, 王丽侠, 王素华, 王小宝. 绿豆基因组SSR引物在豇豆属作物中的通用性[J]. 作物学报, 2012, 38(02): 223-230.

ZHONG Min, CHENG Xu-Zhen, WANG Li-Xia, WANG Su-Hua, WANG Xiao-Bao. Transferability of Mungbean Genomic-SSR Markers in Other Vigna Species[J]. Acta Agron Sin, 2012, 38(02): 223-230.

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链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2012.00223

https://zwxb.chinacrops.org/CN/Y2012/V38/I02/223

参考文献

[1]Zheng Z-J(郑卓杰), Wang S-M(王述民), Zong X-X(宗绪晓). Chinese Legumes (中国食用豆类学). Beijing: Chinese Agriculture Press, 1997. pp 3–6 (in Chinese)

[2]Cheng X-Z(程须珍), Wang S-H(王素华). The Development and Technology Application in Chinese Mungbean Industry (中国绿豆产业发展及科技应用). Beijing: Chinese Science and Technology Agriculture Press, 2002. pp 3–8 (in Chinese)

[3]Han O K, Kaga A, Isemura T, Wang X W, Tomooka N, Vaughan D A. A genetic linkage map for adzuki bean [Vigna angularis(Willd.) Ohwi and Ohashi]. Theor Appl Genet, 2005, 111: 1278–1287

[4]Zhao D(赵丹), Cheng X-Z(程须珍), Wang L-X(王丽侠), Wang S-H(王素华), Ma Y-L(马燕玲). Integration of mungbean (Vigna radiata) genetic linkage map. Acta Agron Sin (作物学报), 2010, 36(6): 932–939 (in Chinese with English abstract)

[5]Wang L-X(王丽侠), Cheng X-Z(程须珍), Wang S-H(王素华), Liang H(梁辉), Zhao D(赵丹), Xu L(徐宁). Genetic diversity of adzuki bean germplasm resources revealed by SSR Markers. Acta Agron Sin (作物学报), 2009, 35(10): 1858–1865 (in Chinese with English abstract)

[6]Fazio G, Chung S M, Staub J E. Comparative analysis of response to phenotypic and marker-assisted selection for multiple lateral branching in cucumber ( Cucumis sativus L.). Theor Appl Genet, 2003, 107: 875–883

[7]Lim G A, Jewell E G, Li X, Erwin T A, Love C, Batley J, Spangenberg G, Edwards D. A comparative map viewer integrating genetic maps for Brassica and Brabidopsis. Plant Biol, 2007, 7: 40–45

[8]Sharma R K, Gupta P, Sharma V, Sood A, Mohapatra T, Ahuja P S. Evaluation of rice and sugarcane SSR markers for phylogenetic and genetic diversity analyses in bamboo. Genome, 2008, 51: 91–103

[9]Wang X-G(王小国), Liang H-Y(梁红艳), Zhang W(张薇). General use analysis in pooideae of SSR marking coming from the wheat gene set. Acta Agric Boreali-Sin (华北农学报), 2007, 22(4):155–157 (in Chinese with English abstract)

[10]Li N(李楠), Zhang H-Y(张海英), Chen N-L(陈年来), Wang Y-J(王永健), Xu Y(许勇), Gong G-Y(宫国义), Guo S-G(郭绍贵). Comparison of versatility and polymorphism of SSRs in three cucurbitaceae. Acta Agric Boreali-Sin (华北农学报), 2008, 23(4): 110–114 (in Chinese with English abstract)

[11]Kumar S V, Tan S G, Quah S C, Yusoff K. Isolation and characterization of seven tetranucleotide microsatellite loci in mungbean(Vigna radiate). Mol Ecol Notes, 2002, 2: 293–295

[12]Kumar S V, Tan S G, Quah S C, Yusoff K. Isolation of microsatellite markers in mungbean, Vigna radiata. Mol Ecol Notes, 2002, 2: 96–98

[13]Miyagi M, Humphry M, Ma Z Y, Lambrides C J, Bateson M, Liu C J. Construction of bacterial artificial chromosome libraries and their application in developing PCR-based markers closely linked to a major locus conditioning bruchid resistance in mungbean. Theor Appl Genet, 2004, 110: 151–156

[14]Gwag J G, Chung J W, Chung H K, Lee J H, Ma K H, Dixit A,Park Y J, Cho E G, Kim T S, Lee S H. Characterization of new microsatellite markers in mungbean (Vigna radiata L). Mol Ecol Notes, 2006, 6: 1132–1134

[15]Xu Y-H(徐雁鸿), Guan J-P(关建平), Zong X-X(宗绪晓). Genetic diversity analysis of cowpea germplasm resources by SSR. Acta Agron Sin (作物学报), 2007, 33(7): 1206–1209 (in Chinese with English abstract)

[16]Xu N(徐宁), Cheng X-Z(程须珍), Wang S-H(王素华), Wang L-X(王丽侠), Zhao D(赵丹). Advances in research on germplasm resources of adzuki bean (Vigna angularis). J Plant Genet Res (植物遗传资源学报), 2008, 9(3): 392–396 (in Chinese with English abstract)

[17]Gupta S K, Gopalakrishna T. Devolopment of unigene-derived SSR markers in cowpea (Vigna unguiculate) and their transferability to other Vigna species. Genome, 2010, 53: 508–523

[18]Kandpal R P, Kandpal G, Weissman S M. Construction of libraries enriched for sequence repeats and jumping clones, and hybridization selection for region-specific markers. Proc Natl Acad Sci USA, 1994, 91: 88–92

[19]Zane L, Bargelloni L, Patarnello T. Strategies for mierosatellite isolation: a review. Mol Ecol, 2002, 11: 1–16

[20]Chen H-Q(陈怀琼), Sui-C(隋春), Wei J-H(魏建和). Summary of strategies for developing SSR primer. Mol Plant Breed (分子植物育种), 2009, 7(4): 845–85l (in Chinese with English abstract)

[21]Doyle J J, Doyle J L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull, 1987, 19: 11–15

[22]Di Gaspero G, Peterlunger E, Testolin R, Ewards K J, Cipriani G. Conservation of microsatellite loci within the genus Vitis. Theor Appl Genet, 2000, 101: 301–308

[23]Rossetto M, Mcnally J, Henry R J. Evaluating the potential of SSR flanking regions for examining taxonomic relationships in Vitaceae. Theor Appl Genet, 2002, 104: 61–66

[24]Wen M-F(文明富), Chen X(陈新), Wang H-Y(王海燕), Lu C(卢诚), Wang W-Q(王文泉). Transferability analysis of cassava EST-SSR and genomic-SSR markers in jatropha and rubber tree. Acta Agron Sin (作物学报), 2011, 37(1): 74–78 (in Chinese with English abstract)

[25]Chen H M, Li L Z, Wei X Y, Li S S, Lei T D, Hu H Z, Wang H G, Zhang X S. Development, chromosome location and genetic mapping of EST-SSR markers in wheat. Chin Sci Bull, 2005, 50: 2328–2336

[26]Li L Z, Wang J J, Guo Y, Jiang F S, Xu Y F, Wang Y Y, Pan H T, Han G Z, Li R J, Li S S. Development of SSR markers from ESTs of gramineous species and their chromosome location on wheat. Prog Nat Sci, 2008, 18: 1485–1490

[27]Wang L-X(王丽侠), Cheng X-Z(程须珍), Wang S-H(王素华), Liu C-Y(刘长友), Liang H(梁辉). Transferability of SSR from adzuki bean to mungbean. Acta Agron Sin (作物学报), 2009, 35(5): 816–820 (in Chinese with English abstract)

[28]Liu C-Y(刘长友), Cheng X-Z(程须珍), Wang S-H(王素华), Wang L-X(王丽侠), Sun L(孙蕾), Mei L(梅丽), Xu N(徐宁). The screening of SSR and STS markers for genetic diversity analysis of mungbean. J Plant Genet Res (植物遗传资源学报), 2007, 8(3): 298–302 (in Chinese with English abstract)

[29]Humphry M E, Konduri V, Lambrides C J, Magner T, McIntyre C L, Aitken E A B, Liu C J. Development of a mungbean [(Vigna radiate L.) Wilczek] RFLP linkage map and its comparison with lablab (Lablab purpureus) reveal a high level of colinearity between the two genomes. Theor Appl Genet, 2002, 105: 160–166

[30]Boutin S R, Young N D, Olsen T C, Yu Z H, Shoemaker R C, Vallejos C E. Genome conservation among three legume genera detected with DNA markers. Genome, 1995, 38: 928–937

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