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作物学报 ›› 2012, Vol. 38 ›› Issue (02): 275-284.doi: 10.3724/SP.J.1006.2012.00275

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

玉米籽粒中花色苷和黑色素含量的QTL分析

覃鸿妮，晏萌，王召辉，郭莹，王辉，孙海燕，刘志斋，蔡一林*   

  1. 西南大学玉米研究所 / 农业部生物技术与作物品质改良重点实验室，重庆400716
  • 收稿日期:2011-04-14 修回日期:2011-09-10 出版日期:2012-02-12 网络出版日期:2011-11-07
  • 通讯作者: 蔡一林, E-mail: caiyilin1789@yahoo.com.cn, Tel: 023-68250447
  • 基金资助:

    本研究由重庆市重大科技专项(CSTC2010AA1022)，中央高校基本科研业务费专项(XDJK2009C122)，国家大学生创新性实验计划项目(091063510)和重庆市主要农作物种质资源共享平台建设项目(CSTC2010AA1033)资助。

QTL Mapping for Anthocyanin and Melanin Contents in Maize Kernel

QIN Hong-Ni,YAN Meng,WANG Zhao-Hui,GUO Ying,WANG Hui,SUN Hai-Yan,LIU Zhi-Zhai,CAI Yi-Lin*   

  1. Maize Research Institute, Southwest University / Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Chongqing 400716, China
  • Received:2011-04-14 Revised:2011-09-10 Published:2012-02-12 Published online:2011-11-07
  • Contact: 蔡一林, E-mail: caiyilin1789@yahoo.com.cn, Tel: 023-68250447

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被引次数

19

摘要： 花色苷和黑色素是黑玉米籽粒中重要而有益的化学成分，深入开展其QTL定位研究，对于色素相关基因的克隆与转化和分子标记辅助育种，具有重要的理论意义和应用价值。本文利用一个黑玉米自交系SDM为共同父本，分别与白玉米自交系木6和黄玉米自交系Mo17杂交，构建2个相关F2:3群体(分别缩写为WD和YD)，对玉米籽粒中花色苷含量(ACK)和黑色素含量(MCK)进行QTL分析。结果表明，黑玉米SDM籽粒中的花色苷和黑色素含量均极显著高于木6和Mo17，2种色素含量间呈极显著正相关。2个群体中共检测到17个色素相关的QTL，其中与花色苷含量相关的QTL在2个群体中各4个，分布在第4、第6、第7和第10染色体上，与黑色素含量相关的QTL在WD和YD群体中分别为4个和5个，分布在第1、第2、第6、第7和第10染色体上。2个群体检测到QTL的数量、分布和对表型的贡献率均高度一致，而且解释花色苷含量和黑色素含量变异大的QTL在2个群体中均有成簇分布的现象，主要表现在bin 6.04处的标记区间umc1796~mmc2006内和bin 10.04处的标记区间umc2043~bnlg1028内，分别解释表型变异的12.7%~21.3%和8.6%~21.3%。它们可能是一因多效的同一QTL或者是在该区段内紧密连锁的不同QTL。上位性分析表明，2个群体中检测到的上位性QTL的数量、位置和对表型的贡献率差别均较大，WD群体的上位性效应明显大于YD群体，说明上位性效应对遗传背景更加敏感，需要进一步深入研究贡献率大的上位性QTL及其利用。

关键词: 玉米籽粒, 花色苷, 黑色素, QTL分析

Abstract: Anthocyanin and melanin are important and beneficial chemical components in black maize kernel. It is important to comprehensively study the QTL mapping for anthocyanin and melanin, cloning and transfer of pigmentation-related genes and marker assistant breeding. In this paper, two related F2:3 populations derived from crosses of Mu6×SDM (WD) and Mo17×SDM (YD) were used to identify QTLs for anthocyanin and melanin contents in maize kernels. The results showed that the phenotype and QTL identification of two populations were highly consistent. The anthocynian and melanin contents of SDM were significantly higher than those of Mu6 and Mo17. The positive correlation between the two pigment contents in kernel was highly significant. A total of seventeen QTLs were identified, including eight QTLs for ACK and nine QTLs for MCK. For ACK, four QTLs were both detected in two populations, which were distributed on chromosomes 4, 6, 7, and 10. For MCK, four and five QTLs were identified in WD and YD, respectively, which were distributed on chromosomes 1, 2, 6, 7, and 10. The number, distribution and effects of QTLs in two populations were highly consistent, and the major QTLs of ACK and MCK in two populations were both clustered, which were detected in the interval umc1796–mmc2006 (bin 6.04) andumc2043–bnlg1028(bins 10.04) and explained 12.7%–21.3% and 8.6%–21.3% of phenotype variation, respectively. These QTLs which were stable in different genetic backgrounds might be closely linked to each other, or a same pleiotropic QTL which could be used in fine mapping and cloning of pigmentation-related genes in maize. The result of epistasic analysis indicated that the number, position and effects of epistatic QTLs between two populations were much different, and epistasic effects were more obvious for WD than for YD. Some epistatic QTL pairs explaining more than 10% variation of phenotype play an important role in pigment production and should be further studied.

Key words: Maize kernel, Anthocyanin, Melanin, QTL mapping

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