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作物学报 ›› 2010, Vol. 36 ›› Issue (09): 1498-1505.doi: 10.3724/SP.J.1006.2010.01498

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆脂肪酸含量的QTL分析

苗兴芬1,2,朱命喜1,徐文平2,丁俊杰4,于凤瑶5,于永梅2,杜升伟1,刘春燕3,陈庆山1,3,*,胡国华1,3,*   

  1. 1东北农业大学农学院，黑龙江哈尔滨150030；2黑龙江农业职业技术学院，黑龙江佳木斯154007；3黑龙江农垦科研育种中心，黑龙江哈尔滨150090；4黑龙江省农科院合江分院，黑龙江佳木斯154007；5黑龙江省红兴隆科研所，黑龙江友谊155811
  • 收稿日期:2009-11-04 修回日期:2010-04-20 出版日期:2010-09-12 网络出版日期:2010-06-11
  • 通讯作者: 陈庆山,E-mail:qshchen@sohu.com;胡国华,E-mail:hugh757@vip.163.com
  • 基金资助:
    本研究由国家转基因生物新品种培育重大专项“大豆导入系构建及有利隐蔽基因挖掘”(2009ZX08009-013B)，国家转基因专项重点项目“多抗、高蛋白、高油转基因大豆种质创制”(2009ZX08004-009B)和黑龙江省教育厅项目(115350033)资助。

QTL Analysis of Fatty acids Contents in Soybean

MIAO Xing-Fen1,2,ZHU Ming-Xi1,XU Wen-Ping2,DING Jun-Jie4,YU Feng-Yao5,YU Yong-Mei2,DU Sheng-Wei1,Chun-Yan3,CHEN Qing-Shan1,3,*,HU Guo-Hua1,3,*   

  1. 1 College of Agriculture, Northeast Agricultural University, Harbin 150030, China; 2 Heilongjiang Agriculture College of Vocational Technology, Jiamusi 154007, China; 3 Land Reclamation Research & Breeding Centre of Heilongjiang, Harbin 150090, China; 4 Jiamusi Branch, Heilongjiang Academy of Agricultural Sciences, Jiamusi 154007, China; 5 Hongxinglong Research Institute of General Bureau of Land Reclamation Heilongjian, Youyi 155811, China
  • Received:2009-11-04 Revised:2010-04-20 Published:2010-09-12 Published online:2010-06-11
  • Contact: CHEN Qing-Shan,E-mail:qshchen@sohu.com;胡国华,E-mail:hugh757@vip.163.com

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被引次数

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摘要： 利用Charleston(♀)×东农594(♂)的F14和F15代永久自交系群体154个单株后代，在两年3点条件下用气相色谱法测得其籽粒5种脂肪酸的含量，利用Win QTL Cartographer 2.5复合区间作图法(CIM)进行QTL分析。结果共检测到47个相关的QTL，分布在13个连锁群上。多年多点同时检测到的QTL共有13个，其中控制软脂酸性状的2个，包括qPal-C2-2和qPal-A1-1；控制硬脂酸性状的４个，包括qSt-B1-1、qSt-B1-2、qSt-D1a-1和qSt-C2-1；控制油酸性状3个，包括qOle-B2-1、qOle-G-1和qOle-H-1；控制亚油酸性状的有2个，包括qLin-C2-1和qLin-H-1；控制亚麻酸性状的4个，包括qLino-B1-1、qLino-C2-1、qLino-D1b-1和qLino-J-1。这些QTL的一致性较高，为特异脂肪酸含量标记辅助育种奠定了基础。大豆脂肪酸含量的主效QTL数量不多, 效应大的不多, 可能还受许多未能检测出来的微效基因控制。

关键词: 大豆, 脂肪酸, QTL分析

Abstract: Soybean, Glycine max (L.) Merr., is one of the main plant oil sources. Most researches have focused on the improvement of oil content. However, improving fatty acid composition is the main aspect of soybean breeding now. This study aimed at mapping QTLs conferring fatty acids contents in soybean on the genetic map which had been constructed in our lab to provide a reference of soybean oil quality breeding. Five kinds of fatty acid contents of 154 F14 and F15 lines devisedqSt-B1-1, qSt-B1-2, qSt-D1a-1 and qSt-C2-1;three for related with oleic acid content, i.e. qOle-B2-1, qOle-G-1, and qOle-H-1; two for related with linoleic acid content, i.e. qLin-C2-1 and qLin-H-1, and four for linolenic acid content, i.e. qLino-B1-1,qLino-C2-1, qLino-D1b-1, and qLino-J-1. These stable QTLs pave a way for specific fatty acid contents breeding by marker-assisted selection. The number of main-effect QTLs with large effect conferring the contents of fatty acids appeared not as many as expected, it may also subject to a number of the micro-effect genes which failed to detect. from a cross of Charleston (♀) × Dongnong 594 (♂) were measured by GC at three locations in two years. The QTLs of fatty acids contents were analyzed with method of composite interval mapping (CIM) by Win QTL Cartographer 2.5. In total, 47 related QTLs distributed on the 13 linkage groups were obtained. Seventeen QTLs were detected in different years or different sites, including two for palmitic acid content, i.e. qPal-C2-2 and qPal-A1-1; four for stearic acid content, i.e.

Key words: Soybean, Fatty acids contents, QTL analysis

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