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作物学报 ›› 2012, Vol. 38 ›› Issue (04): 596-605.doi: 10.3724/SP.J.1006.2012.00596

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

国家芝麻区域试验新品种(系)的DNA指纹分析

刘红艳，吴坤，杨敏敏，左阳，赵应忠*   

  1. 中国农业科学院油料作物研究所 / 农业部油料作物生物学与遗传育种重点实验室，湖北武汉 430062
  • 收稿日期:2011-08-17 修回日期:2011-12-19 出版日期:2012-04-12 网络出版日期:2012-02-13
  • 通讯作者: 赵应忠, E-mail: yingzhzh@public.wh.hb.cn
  • 基金资助:

    本研究由国家自然科学基金项目(31101180)，中国农业科学院油料作物研究所所长基金项目(1610172011007)，国家芝麻产业技术体系项目(CARS-15)和国家科技支撑计划项目(2009BADA8B04)资助。

DNA Fingerprinting of Sesame (Sesamum indicum L.) Varieties (Lines) from Recent National Regional Trials in China

LIU Hong-Yan,WU Kun,YANG Min-Min,ZUO Yang,ZHAO Ying-Zhong*   

  1. Key Laboratory of Oil Crops Biology and genetic breeding of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Science, Wuhan 430062, China
  • Received:2011-08-17 Revised:2011-12-19 Published:2012-04-12 Published online:2012-02-13
  • Contact: 赵应忠, E-mail: yingzhzh@public.wh.hb.cn

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1499

被引次数

23

摘要： 以我国近年参加国家芝麻区域试验的43个品种(系)为材料，利用12对SSR核心引物开展DNA指纹分析，初步构建参试品种的DNA指纹图谱，并根据指纹图谱分析了参试品种的特异性和一致性。聚类分析结果表明，12对引物检测到30个标记，可将43个品种完全区分开，来源于同一育种单位的品种遗传基础相近，而不同来源的品种其遗传背景相差较大；以遗传相似系数0.90为划分标准，95%的供试品种具备特异性；以引物位点的一致性为指标，80%的品种具有一致性；分配试验中，86%的单株都能正确地分配回到原来的品种，这些品种的一致性较好，个别品种的单株正确分配率较低，品种一致性差。表明大部分参加国家区试的品种都具有很好的特异性和一致性。

关键词: 芝麻品种, SSR, 特异性, 一致性

Abstract: DNA fingerprinting is an important technique for plant variety identification and protection. In this study, 43 sesame (Sesamum indicum L.) varieties (lines) from recent National Regional Trail were amplificated with 12 pairs of SSR primers, and a total of 30 polymorphic bands were obtained. These molecular markers were further used for variety (lines) distinction and uniformity analysis. Clustering analysis showed that 43 varieties (lines) were completely differed from each other with 30 markers. Varieties (lines) from the same breeding institute tended to share a higher genetic similarity, while varieties (lines) with different origins seemed to be more distinct. With a genetic similarity coefficient threshold of 0.90, 90% of varieties (lines) were distinctive, and 80% of tested varieties (lines) were classified into high uniformity group in consistence with marker loci uniformity, Furthermore, in assignment test, 86% of derivative individuals were correctly corresponding to their original varieties (lines), it indicated that most varieties (lines) have a good uniformity. Overall, the results showed that most of the tested sesame varieties (lines) have good distinction and uniformity, and SSR marker is suitable for variety identification and protection.

Key words: Sesame variety, SSR, Distinctness, Uniformity

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