作物学报 ›› 2013, Vol. 39 ›› Issue (06): 1096-1104.doi: 10.3724/SP.J.1006.2013.01096
杨东清,王振林*,尹燕枰,倪英丽,杨卫兵,蔡铁,彭佃亮,徐彩龙,崔正勇,刘铁宁,徐海成
YANG Dong-Qing,WANG Zhen-Lin*,YIN Yan-Ping,NI Ying-Li,YANG Wei-Bing,CAI Tie,PENG Dian-Liang,XU Cai-Long,CUI Zheng-Yong,LIU Tie-Ning,XU Hai-Cheng
摘要:
为深入认识植物激素在小麦抗氧化和调控衰老中的作用机制,以持绿品种汶农6号和非持绿品种济麦20为材料,在盛花期后喷施脱落酸(ABA)和6-苄基腺嘌呤(6-BA),考察外源激素对旗叶衰老过程中生理生化指标动态变化以及籽粒产量的影响,并探讨了激素与衰老的关系。结果表明,汶农6号旗叶的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性、叶绿素含量及籽粒千粒重和产量均大于济麦20,且丙二醛(MDA)含量低于济麦20,表明持绿型品种抗氧化能力强,衰老进程较慢,进而获得较高产量。外源ABA和6-BA处理显著提高花后7~28 d旗叶叶绿素含量,提高花后21~35 d可溶性蛋白含量,显著降低花后28~35 d旗叶MDA含量。外源ABA降低两品种旗叶玉米素(ZR)含量,但提高生长素(IAA)和赤霉素(GA)含量,降低了其在花后28~35 d的ABA含量。6-BA处理提高旗叶ZR含量及花后7~14 d IAA含量,降低济麦20花后21~35 d旗叶ABA含量。外源ABA显著提高汶农6号花后7~21 d旗叶SOD活性,喷施ABA对汶农6号旗叶POD和过氧化氢酶(CAT)活性影响没有显著性影响,但显著提高了济麦20花后7~28 d POD活性,喷施6-BA提高了两品种7~28 d SOD、POD和CAT活性。总之,ABA和6-BA处理改变了旗叶内源激素水平,提高抗氧化酶活性,降低MDA含量,延缓旗叶衰老,从而提高了籽粒产量。
| [1]Yan C-S(阎成士), Li D-Q(李德全), Zhang J-H(张建华). Plant leaf senescence and oxidative stress. Chin Bull Bot (植物学通报), 1999, 16(4): 398–404 (in Chinese with English abstract)[2]Li H(李红), Tang M-F(唐明凤), Yu F(喻凤), Luo P-G(罗培高). Determination the influence of removing flag leaf during different stages afer heading on wheat yield. Acta Agric Boreali-Sin (华北农学报), 2010, 25(12): 93–97 (in Chinese with English abstract)[3]Duan L-S(段留生), Han B-W(韩碧文), He Z-P(何钟佩). The effects of correlation between leaf and other organs on leaf senescence. Chin Bull Bot (植物学通报), 1998, 15(1): 43–49 (in Chinese with English abstract)[4]Xu Z-H(许智宏), Li J-Y(李家洋). Plant hormones research in China: past, present and future. Chin Bull Bot (植物学通报), 2006, 23(5): 433–442 (in Chinese with English abstract)[5]Guo W-Q(郭文琦), Chen B-L(陈兵林), Liu R-X(刘瑞显), Zhou Z-G(周治国). Effect of nitrogen application rate on cotton leaf antioxidant enzyme activities and endogenous hormone contents under short-term waterlogging at flowering and boll-forming stage. Chin J Appl Ecol(应用生态学报), 2010, 21(1): 53–60 (in Chinese with English abstract)[6]Wu Y-R(吴耀荣), Xie Q(谢旗). ABA and plant stress response. Chin Bull Bot (植物学通报), 2006, 23(5): 511–518 (in Chinese with English abstract)[7]Aroca R, Vernieri P, Irigoyen J J, Sanchez M, Tognoni F, Pardossi A. Involvement of abscisic acid in leaf and root of maize (Zea mays L.) in avoiding chilling-induced water stress. Plant Sci, 2003, 165: 671–679[8]Zhu Z-H(朱中华), Duan L-S(段留生), Feng X-M(冯雪梅), Han B-W(韩碧文), He Z-P(何钟佩). Systemmatical analysis of hormones regulation on wheat leaves senescence. Acta Agron Sin (作物学报), 1998, 24(2): 176–181 (in Chinese with English abstract)[9]Hu X L, Jiang M Y, Zhang A Y, Lu J. Abscisic acid-induced apoplastic H2O2 accumulation up-regulates the activities of chloroplastic and cytosolic antioxidant enzymes in maize leaves. Planta, 2005, 223: 57–68[10]Huang F-L(黄凤莲), Dai L-Y(戴良英), Luo K(罗宽). Mechanism studies on pharmaceutical induction for resistance to cold of rice seedlings. Acta Agron Sin (作物学报), 2000, 26(1): 92–97 (in Chinese with English abstract)[11]Travaglia C, Reinoso H, Cohen A, Luna C, Tommasino E, Castillo C, Bottini R. Exogenous ABA increases yield in field-grown wheat with moderate water restriction. J Plant Growth Regul, 2010, 29: 366–374[12]Lin D-B(林定波), Liu Z-Q(刘祖棋). Effect of cold acclimation and ABA on membrane stability and synthesis of membrane protein in citrus. J Nanjing Agric Univ (南京农业大学学报), 1994, 17(1): 1–5 (in Chinese with English abstract)[13]Jiang H(姜华), Wan J(万佳), Gao X-L(高晓玲), Chen J(陈静), Wang X-D(王旭东), Xu Z-J(徐正军). Purification and analysis of abscisic acid-specifically-inducible protein from rice callus. Chin J Rice Sci (中国水稻科学), 2006, 20(6): 583–588 (in Chinese with English abstract)[14]Zhang H-N(张海娜), Li C-D(李存东), Xiao K(肖凯). Regulation effects of exogenous 6-BA on photosynthesis and leaf senescence in cotton. Cott Sci (棉花学报), 2007, 19(6): 467–471 (in Chinese with English abstract)[15]Liu X, Huang B. Cytokinin effects on creeping bentgrass response to heat stress: II. Leaf senescence and antioxidant metabolism. Crop Sci, 2002, 42:466–473[16]Shao R-X(邵瑞鑫), Li J(李建), Xin L-F(信龙飞), Zhao Y(赵宇), Yang Q-H(杨青华). Effects of kinetin and succinic acid seed dressing on the ultrastructure of chloroplasts and chlorophyll fluorescence parameters in aging process of maize. Plant Nutr Fert Sci (植物营养与肥料学报), 2012, 18(6): 1362–1369 (in Chinese with English abstract)[17]Wang Y-R(王彦荣), Hua Z-T(华泽田), Chen W-F(陈温福), Dai G-J(代贵金), Hao X-B(郝宪彬), Wang Y(王岩), Zhang Z-X(张忠旭), Sui G-M(隋国民). Relation between root and leaf senescence and their effects on grain-filling in japonica rice. Acta Agron Sin (作物学报), 2003, 29(6): 892–898 (in Chinese with English abstract)[18]Thomas H, Howarth C J. Five ways to stay green. J Exp Bot, 2000, 51: 329–337[19]Liu Y(刘洋), Shi H-Q(石慧清), Gong Y-H(龚月桦). Effects of sulfur and nitrogen fertilizer combination on nitrogen transfer and leaf senescence in stay-green wheat. Acta Acta Bot Boreali-Occident Sin (西北植物学报), 2012, 32(6): 1206–1213 (in Chinese with English abstract)[20]Luo P-G(罗培高), Ren Z-L(任正隆), Wu X-H(吴先华), Zhang H-Y(张怀渝), Zhang H-Q(张怀琼), Feng J(冯娟). The structure and physiological and biochemical mechanism of delaying senescence of wheat. Chin Sci Bull (科学通报), 2006, 51(18): 2154–2460 (in Chinese)[21]Zhao S-J(赵世杰), Shi G-A(史国安), Dong X-C(董新纯). Experimental Guide for Plant Physiology (植物生理实验指导). Beijing: China Agricultural Science and Technology Press, 1998. pp 55–60 (in Chinese)[22]Read S M, Northeote D H. Minimization of variation in the response to different protein of the coomassic blue G dye-binding assay for protein. Anal Biochem, 1981, 116: 53–64[23]Zhao S-J(赵世杰), Xu C-C(许长城), Zou Q(邹琦), Meng Q-W(孟庆伟). The improvement of MDA measurement in plant. Plant Physiol Commun (植物生理学通讯), 1994, 30(3): 207–210 (in Chinese)[24]Yang W-B(杨卫兵), Wang Z-L(王振林), Yin Y-P(尹燕枰), Li W-Y(李文阳), Li Y(李勇), Chen X-G(陈晓光), Wang P(王平), Chen E-Y(陈二影), Guo J-X(郭俊祥), Cai T(蔡铁), Ni Y-L(倪英丽). Effects of spraying exogenous ABA or GA on the endogenous hormones concentration and filling of wheat grains. Sci Agric Sin (中国农业科学), 2011, 44(13): 2673–2682 (in Chinese with English abstract)[25]Zou Q(邹琦). Experimental of Plant Physiology (植物生理学实验指导). Beijing: China Agriculture Press, 2000 (in Chinese)[26]Xue H(薛晖), Jia L(贾丽), Gong Y-H(龚月桦), Liu Y-Z(刘赢洲), Wu Y-H(吴养会). Study on the stay-green capacity and leaf senescence of winter wheat. Acta Bot Boreali-Occident Sin (西北植物学报), 2010, 30(2): 336–343 (in Chinese with English abstract)[27]Liu L-T(刘连涛), Li C-D(李存东), Sun H-C(孙红春), Zhang Y-J(张永江), Bai Z-Y(白志英), Feng L-X(冯丽肖). Effects of nitrogen on cotton senescence and the corresponding physiological mechanisms. Sci Agric Sin (中国农业科学), 2009, 42(5): 1575–1581 (in Chinese with English abstract)[28]Wang Y-X(汪月霞), Suo B(索标), Zhao P-F(赵鹏飞), Qu X-F(取小菲), Yuan L-G(袁利刚), Zhao X-J(赵雪娟), Zhao H-J(赵会杰). Effect of abscisic acid treatment on psbA gene expression in two wheat cultivars during grain filling stage under drought stress. Acta Agron Sin (作物学报), 2011, 37(8): 1372–1377 (in Chinese with English abstract)[29]Sun Z-Y(孙振元), Duan L-S(段留生), Han B-W(韩碧文), Liu S-L(刘淑兰). Stereological changes of chloroplast ulteastructure and the effects of 6-benzylaminopurine during wheat leaf senescence. Acta Agron Sin (作物学报), 1998, 24(6): 818–820 (in Chinese with English abstract)[30]Wang Y-F(王月福), Yu Z-W(于振文), Li S-X(李尚霞), Yu S-L(余松烈). Effect of nitrogen nutrition on protein and nucleic acid metabolism during senescence of flag leaf. Plant Nutr Fert Sci (植物营养与肥料学报), 2003, 9(2): 178–183 (in Chinese with English abstract)[31]Yu H(鱼欢), Feng B-L(冯佰利), Zhang Y(张英), Liu P-T(刘鹏涛), He Y-Y(何永艳), Dai H-P(代惠萍), Li S-X(李生秀). Leaf aging and reactive oxygen species metabolism of winter wheat in different cultivation modes. Acta Agron Sin (作物学报), 2007, 33(10): 1729–1732 (in Chinese with English abstract)[32]Zhang W-W(张微微), Huang X-L(黄晓林), Zhao X(赵霞), Chen S-B(陈胜兵), Rong L(荣利), Chen X(陈旭), Tang Y-Z(汤一卒). Research progress on enzymatic antioxidant system response to stress in cotton. Chin Agric Sci Bull (中国农学通报), 2009, 25(19): 108–112 (in Chinese with English abstract)[33]Wang H(王虹), Jiang Y-P(姜玉萍), Shi K(师恺), Zhou Y-H(周艳虹), Yu J-Q(喻景权). Effects of light quality on leaf senescence and activities of antioxidant enzymes in cucumber plants. Sci Agric Sin (中国农业科学), 2010, 43(3): 529–534 (in Chinese with English abstract)[34]Zhu D(朱丹), Jiang M-Y(蒋明义), Tan M-P(谭明普). The mechanism of ABA-induced apoplastic H2O2 accumulation in maize leaves. J Plant Physiol Mole Biol (植物生理与分子生物学学报), 2006, 32(5): 519–526 (in Chinese with English abstract)[35]Xu S-C(许树成), Ding H-D(丁海东), Lu R(鲁锐), Shi B(石犇), Ma F-F(马芳芳). Study on effects of ABA in antioxidant defense of plant cells. J China Agric Univ (中国农业大学学报), 2008, 13(2): 11–19 (in Chinese with English abstract)[36]Hironori K, Shigeto M, Megumi T, Takehiro M, Kunisuke T. Differential gene expressions of rice superoxide dismutase isoforms to oxidative and environmental stresses. Free Radical Res, 1999, 31: 219–255[37]Xu S C, Ding H D, Su F X, Zhang A Y, Jiang M Y. Involvement of protein phosphorylation in water stress-induced antioxidant defense in maize leaves. J Integr Plant Biol, 2009, 51: 654–662[38]Zhang H-N(张海娜), Li C-D(李存东), Xiao K(肖凯). The physiological mechanism of plant growth and senescence characteristics in wheat regulated by exogenous cytokinin. Acta Agric Boreali-Sin (华北农学报), 2007, 22(5): 1–4 (in Chinese with English abstract)[39]Wang S-G(王三根). Roles of cytokinin on stress-resistance and delaying senescence in plants. Chin Bull Bot (植物学通报), 2000, 17(2): 121–126 (in Chinese with English abstract)[40]He P(何萍), Jin J-Y(金继云). Relationships among hormone changes, transmembrane Ca2+ flux and lipid peroxidation during leaf senescence in spring maize. Acta Bot Sin (植物学报), 1999, 41(11): 1221–1225 (in Chinese with English abstract)[41]Zheng S-S(郑莎莎), Sun C-F(孙传范), Sun H-C(孙红春), Liu L-T(刘连涛), Zhao J-F(赵金峰), Li C-D(李存东). Effects of different exogenous hormones on physiological characteristics of main stem leaves at flower and boll stage in cotton. Sci Agric Sin (中国农业科学), 2009, 42(12): 4383–4389 (in Chinese with English abstract)[42]Xiao K(肖凯), Zhang R-X(张荣铣), Qian W-P(钱维朴). The physiological mechanism of senescence and photosynthetic function decline of flag leaf in wheat regulated by nitrogen nutrition. Plant Nutr Fert Sci (植物营养与肥料学报), 1998, 4(4): 371–378 (in Chinese with English abstract)[43]Bao F(包方), Hu Y-X(胡玉欣), Li J-Y(李家洋). Identification of auxin responsive genes in arabidopsls by cDNA array. Chin Sci Bull (科学通报), 2001, 46(23): 1988–1992 (in Chinese)[44]Wang D(王丹), Fan S-L(范术丽), Song M Z(宋美珍), Pang Z-Y(庞朝友), Yu S-X(喻树迅). The effect of gibberellm on leaf senescenle-associated genes. China Cott (中国棉花), 2011, 38(3): 11–13 (in Chinese with English abstract)[45]Huang S(黄森), Zhang J-S(张继澍), Zhang Y-M(张院民). Effect of GA3 treatment on ethylene biosynthesis in post-harvested persimmon fruits. Chin Agric Sci Bull (中国农学通报), 2006, 22(2): 88–90 (in Chinese with English abstract)[46]Wang Z(王忠). Plant Physiology (植物生理学). Beijing: China Agriculture Press, 2006. pp 424–425 (in Chinese)[47]Wang X-Y(王晓云), Zou Q(邹琦). Advances in studies on relationship between polyamines and plant senescence. Chin Bull Bot (植物学通报), 2002, 19(1): 11–20 (in Chinese with English abstract)[48]Zheng D-F(郑殿峰), Zhao L-M(赵黎明), Feng N-J(冯乃杰). Effects of plant growth regulators (PGRs) on endogenous hormone contents and activities of protective enzymes in soybean leaves. Acta Agron Sin (作物学报), 2008, 34(7): 1233–1239 (in Chinese with English abstract) |
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