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作物学报 ›› 2013, Vol. 39 ›› Issue (08): 1462-1468.doi: 10.3724/SP.J.1006.2013.01462

• 耕作栽培·生理生化 • 上一篇    下一篇

剪根与水分胁迫对小麦单根和细胞导水率及TaPIP基因表达的影响

王卫锋1,3,杨晓青1,3,张岁岐1,2,*,山仑1,2   

  1. 1中国科学院水利部水土保持研究所 / 黄土高原土壤侵蚀与旱地农业国家重点实验室,陕西杨凌 712100; 2西北农林科技大学,陕西杨凌 712100;3中国科学院研究生院,北京 100049
  • 收稿日期:2012-12-19 修回日期:2013-03-11 出版日期:2013-08-12 网络出版日期:2013-04-23
  • 通讯作者: 张岁岐, E-mail: sqzhang@ms.iswc.ac.cn, Tel: 029-87010897
  • 基金资助:

    本研究由国家自然科学基金项目(30971714), 国家重点基础研究发展计划(973计划)项目(2009CB1186604)和教育部高等学校学科创新引智计划(B12007)项目资助。

Effects of Root Excision and Water Stress on Root Hydraulics and TaPIPs Expression in Wheat Seedlings

WANG Wei-Feng1,3,YANG Xiao-Qing1,3,ZHANG Sui-Qi1,2,*,SHAN Lun1,2   

  1. 1 State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau / Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, 712100, China; 2 Northwest A&F University, Yangling 712100, China; 3 Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2012-12-19 Revised:2013-03-11 Published:2013-08-12 Published online:2013-04-23
  • Contact: 张岁岐, E-mail: sqzhang@ms.iswc.ac.cn, Tel: 029-87010897

摘要:

剪去小麦部分根系能瞬间打破其水分平衡,研究根系导水特性对剪根的响应有助于解释静水压对作物根系吸水的调节机制。通过对苗期小麦(Triticum aestivum)剪根与水分胁迫处理,用压力探针技术测定单根和细胞两种尺度上的根导水特性变化,以及根中TaPIP1;2TaPIP2;5的转录调节变化。结果显示,剪根处理或水分胁迫处理使叶片蒸腾速率和气孔导度均显著低于对照,而单根导水率和细胞导水率均与对照无显著差异。剪根处理的叶片蒸腾速率、气孔导度、叶水势、单根导水率和细胞导水率均显著高于水分胁迫处理,而剪根且水分胁迫处理的各参数均显著低于其他处理。各处理的单根导水率与细胞导水率显著正相关。各处理根中TaPIP1;2TaPIP2;5相对mRNA含量的变化规律与单根和细胞导水率的变化规律相似。剪根处理显著上调了TaPIP1;2TaPIP2;5转录,水分胁迫处理显著下调了其转录,但TaPIP1;2TaPIP2;5在剪根且水分胁迫处理中的转录水平最低。这些结果表明,小麦的根导水特性在单根尺度和细胞尺度上具有一致性;剪根短期内能够增加小麦幼苗的水分敏感性。推测TaPIP1;2TaPIP2;5参与了静水压对小麦根导水特性的调节过程。

关键词: 小麦, 剪根, 水分胁迫, 单根导水率, 细胞导水率, TaPIPs

Abstract:

Root excision can break water balance between the water uptake by roots and the transpriation in shoots and induce hydraulic responses of roots, which is helpful to explain how hydrostatic gradient regulates root hydraulic traits. To explore the regulation mechanisms of root hydraulics to the broken whole plant water balance by root excision and water stress, we investigated the changes of individual root (Lproot) and cortex cell (Lpcell) hydraulic conductivities of wheat (Triticum aestivum, cv. Changwu 134) after root excision and water stress with root and cell pressure probes. The transcription levels of TaPIP1;2 and TaPIP2;5 were also measured with Quantitative Real-time PCR. Root excision or water stress treatment significantly reduced the leaf transpiration rate and stomatal conductance of wheat seedlings, but the Lproot or Lpcell of the remained roots had no significant changes. The transpiration rate, stomatal conductance, leaf water potential, Lproot, and Lpcell of root-excised plants were significantly higher than those of plants under water stress. However, these parameters of plants with both root excision and water stress were significantly lower than those with a single treatment or the control. Irrespectively of root excision or water stress, Lpcell had a significantly positive correlation with Lproot (R2 = 0.97, P < 0.05), which suggested the accordance in root water uptake ability at cell level and individual root level. Root excision significantly up-regulated the relative mRNA contents of TaPIP1;2 and TaPIP2;5 in wheat roots, but water stress had an effect of down-regulation. The relative mRNA contents of TaPIP1;2 and TaPIP2;5 of root excised and water stressed seedlings were the lowest in the four treatments. These results may suggest that root excision could reduce the tolerance of wheat seedling to the following water stress; cortex cell hydraulic conductivity could be accordant with individual root hydraulic conductivity during wheat root hydraulics regulation; and TaPIP1;2 and TaPIP2;5 may be involved in regulating root hydraulics of wheat.

Key words: Wheat, Root excision, Water stress, Individual root hydraulic conductivity, Cell hydraulic conductivity, TaPIPs

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