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作物学报 ›› 2013, Vol. 39 ›› Issue (08): 1452-1461.doi: 10.3724/SP.J.1006.2013.01452

• 耕作栽培·生理生化 • 上一篇    下一篇

玉米果穗不同部位籽粒激素含量及其与胚乳发育和籽粒灌浆的关系

徐云姬,顾道健,张博博,张耗,王志琴,杨建昌*   

  1. 扬州大学农学院江苏省作物遗传生理重点实验室, 江苏扬州 225009
  • 收稿日期:2013-01-21 修回日期:2013-04-22 出版日期:2013-08-12 网络出版日期:2013-05-20
  • 通讯作者: 杨建昌, E-mail: jcyang@yzu.edu.cn, Tel: 0514-87979317
  • 基金资助:

    本研究由国家重点基础研究发展计划(973计划)项目(2012CB114306), 国家自然科学基金项目(31271641),中央级科研院所基本科研业务费专项基金项目(农业) (201103003, 201203079), 国家“十二五”科技支撑计划项目(2011BAD16B14)和江苏高校优势学科建设工程专项经费资助。

Hormone Contents in Kernels at Different Positions on an Ear and Their Relationship with Endosperm Development and Kernel Filling in Maize

XU Yun-Ji,GU Dao-Jian,ZHANG Bo-Bo,ZHANG Hao,WANG Zhi-Qin,YANG Jian-Chang*   

  1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Agriculture, Yangzhou University, Yangzhou 225009, China
  • Received:2013-01-21 Revised:2013-04-22 Published:2013-08-12 Published online:2013-05-20
  • Contact: 杨建昌, E-mail: jcyang@yzu.edu.cn, Tel: 0514-87979317

摘要:

以玉米品种登海11为材料, 分别进行大田和温室试验, 观察灌浆期果穗不同部位籽粒玉米素(Z)+玉米素核苷(ZR)、吲哚-3-乙酸(IAA)脱落酸(ABA)和赤霉素(GA3)含量变化及其与胚乳发育和籽粒灌浆的关系。结果显示, 籽粒最大胚乳细胞数目、最大胚乳细胞增殖速率及平均速率、最大灌浆速率、平均灌浆速率和百粒重表现为果穗下部籽粒>中部籽粒>上部籽粒。在胚乳细胞活跃增殖期或活跃灌浆期, 籽粒Z+ZRIAAABA含量以果穗下部籽粒最高, 中部籽粒其次, 上部籽粒最低。GA3含量则为果穗上部籽粒>中部籽粒>下部籽粒。两个试验的结果趋势一致。胚乳细胞增殖速率和籽粒灌浆速率与籽粒Z+ZRIAAABA含量呈极显著正相关, 与籽粒GA3含量呈显著负相关。说明玉米果穗上部籽粒轻主要是由于这些籽粒的胚乳细胞增殖速率小, 导致其胚乳细胞数少, 这与其灌浆期较低的Z+ZRIAAABA含量及较高的GA3含量有密切关系。

关键词: 玉米, 果穗不同部位籽粒, 激素, 胚乳细胞, 灌浆

Abstract:

Kernels at the upper position of a maize ear usually show poorer filling and lower weight in contrast to those at the basal position. The mechanism has not been understood. The experiments were conducted using a maize cultivar Denghai 11, grown in both field and greenhouse conditions, to determine number of endosperm cells, kernel filling rate, and contents of zeatin (Z) + zeatin riboside (ZR), indole-3-acetic acid (IAA), abscisic acid (ABA), and gibberellin-3 (GA3) in the kernels at different positions on an ear from silking to maturity. The results indicated that the maximum number of endosperm cells, maximum division rate of endosperm cells,mean endosperm cell division rate,maximumkernel filling rate, mean kernel filling rate and 100-kernel weight for kernels showed an order of basal position > middle position> upper position. During the active endosperm development and the active kernel filling period, contents of Z+ZR, IAA and ABA in kernels were the greatest at the basal position, the mediate at the middle position, and the least at the upper position on an ear. The two experiments exhibited similar results. The endosperm cell division rate and kernel filling rate were very significantly and positively correlated with contents of Z+ZR, IAA, and ABA in kernels, whereas significantly and negatively correlated with content of GA3. The results suggested that a lower kernel weight at the upper position on a maize ear is mainly due to a smaller division rate of endosperm cells, leading to less number of endosperm cells, which is closely associated with lower contents of Z+ZR, IAA, and ABA, as well as higher content of GA3 in the kernels during kernel filling.

Key words: Maize, Kernels at different positions on an ear, Hormone, Endosperm cell, Kernel filling

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