欢迎访问作物学报,今天是

作物学报 ›› 2013, Vol. 39 ›› Issue (08): 1452-1461.doi: 10.3724/SP.J.1006.2013.01452

• 耕作栽培·生理生化 • 上一篇    下一篇

玉米果穗不同部位籽粒激素含量及其与胚乳发育和籽粒灌浆的关系

徐云姬,顾道健,张博博,张耗,王志琴,杨建昌*   

  1. 扬州大学农学院江苏省作物遗传生理重点实验室, 江苏扬州 225009
  • 收稿日期:2013-01-21 修回日期:2013-04-22 出版日期:2013-08-12 网络出版日期:2013-05-20
  • 通讯作者: 杨建昌, E-mail: jcyang@yzu.edu.cn, Tel: 0514-87979317
  • 基金资助:

    本研究由国家重点基础研究发展计划(973计划)项目(2012CB114306), 国家自然科学基金项目(31271641),中央级科研院所基本科研业务费专项基金项目(农业) (201103003, 201203079), 国家“十二五”科技支撑计划项目(2011BAD16B14)和江苏高校优势学科建设工程专项经费资助。

Hormone Contents in Kernels at Different Positions on an Ear and Their Relationship with Endosperm Development and Kernel Filling in Maize

XU Yun-Ji,GU Dao-Jian,ZHANG Bo-Bo,ZHANG Hao,WANG Zhi-Qin,YANG Jian-Chang*   

  1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Agriculture, Yangzhou University, Yangzhou 225009, China
  • Received:2013-01-21 Revised:2013-04-22 Published:2013-08-12 Published online:2013-05-20
  • Contact: 杨建昌, E-mail: jcyang@yzu.edu.cn, Tel: 0514-87979317

摘要:

以玉米品种登海11为材料, 分别进行大田和温室试验, 观察灌浆期果穗不同部位籽粒玉米素(Z)+玉米素核苷(ZR)、吲哚-3-乙酸(IAA)、脱落酸(ABA)和赤霉素(GA3)含量变化及其与胚乳发育和籽粒灌浆的关系。结果显示, 籽粒最大胚乳细胞数目、最大胚乳细胞增殖速率及平均速率、最大灌浆速率、平均灌浆速率和百粒重表现为果穗下部籽粒>中部籽粒>上部籽粒。在胚乳细胞活跃增殖期或活跃灌浆期, 籽粒Z+ZR、IAA和ABA含量以果穗下部籽粒最高, 中部籽粒其次, 上部籽粒最低。GA3含量则为果穗上部籽粒>中部籽粒>下部籽粒。两个试验的结果趋势一致。胚乳细胞增殖速率和籽粒灌浆速率与籽粒Z+ZR、IAA和ABA含量呈极显著正相关, 与籽粒GA3含量呈显著负相关。说明玉米果穗上部籽粒轻主要是由于这些籽粒的胚乳细胞增殖速率小, 导致其胚乳细胞数少, 这与其灌浆期较低的Z+ZR、IAA和ABA含量及较高的GA3含量有密切关系。

关键词: 玉米, 果穗不同部位籽粒, 激素, 胚乳细胞, 灌浆

Abstract:

Kernels at the upper position of a maize ear usually show poorer filling and lower weight in contrast to those at the basal position. The mechanism has not been understood. The experiments were conducted using a maize cultivar Denghai 11, grown in both field and greenhouse conditions, to determine number of endosperm cells, kernel filling rate, and contents of zeatin (Z) + zeatin riboside (ZR), indole-3-acetic acid (IAA), abscisic acid (ABA), and gibberellin-3 (GA3) in the kernels at different positions on an ear from silking to maturity. The results indicated that the maximum number of endosperm cells, maximum division rate of endosperm cells,mean endosperm cell division rate,maximumkernel filling rate, mean kernel filling rate and 100-kernel weight for kernels showed an order of basal position > middle position> upper position. During the active endosperm development and the active kernel filling period, contents of Z+ZR, IAA and ABA in kernels were the greatest at the basal position, the mediate at the middle position, and the least at the upper position on an ear. The two experiments exhibited similar results. The endosperm cell division rate and kernel filling rate were very significantly and positively correlated with contents of Z+ZR, IAA, and ABA in kernels, whereas significantly and negatively correlated with content of GA3. The results suggested that a lower kernel weight at the upper position on a maize ear is mainly due to a smaller division rate of endosperm cells, leading to less number of endosperm cells, which is closely associated with lower contents of Z+ZR, IAA, and ABA, as well as higher content of GA3 in the kernels during kernel filling.

Key words: Maize, Kernels at different positions on an ear, Hormone, Endosperm cell, Kernel filling

[1]State Administration of Grain, P. R. China (国家粮食局). http://nongye.cntv.cn/20121017/102023.shtml



[2]Dong M-H(董明辉), Zhao B-H(赵步洪), Wu X-Z(吴翔宙), Chen T(陈涛), Yang J-C(杨建昌). Difference in hormonal content and activities of key enzymes in the grains at different positions on a rice panicle during grain filling and their correlations with rice qualities. Sci Agric Sin (中国农业科学), 2008, 41(2): 370–380 (in Chinese with English abstract)



[3]Ren Y-M(任亚梅), Liu X-H(刘兴华), Yuan C-L(袁春龙), Zheng J-M(郑建梅), Luo A-W(罗安伟). Study on the carbohydrate change at different parts of fresh unripe maize ears. J Food Sci Biotechnol (食品与生物技术学报), 2005, 24(5): 66–70 (in Chinese with English abstract)



[4]Tang Q-F(唐祈福), Rong T-Z(荣廷昭). The relationship between endogenous hormone and barren ear tip of maize. J Nucl Agric Sci (核农学报), 2007, 21(4): 366–368 (in Chinese with English abstract)



[5]Sikder H P, Gupta D K D. Physiology of grain in rice. Indian Agric, 1976, 20: 133–141



[6]Wang Y. Effectiveness of supplied nitrogen at the primordial panicle stage on rice characteristics and yields. Int Rice Res Newslettt, 1981, 6: 23–24



[7]Murty P S S, Murty K S. Spikelet sterility in relation to nitrogen and carbohydrate contents in rice. Indian J Plant Physiol, 1982, 25: 40–48



[8]Mohapatra P K, Patel R, Sahu S K. Time of flowering affects grain quality and spikelet partitioning within the rice panicle. Aust J Plant Physiol, 1993, 20: 231–242



[9]Kato T. Effect of spikelet removal on the grain filling of Akenohoshi, a rice cultivar with numerous spikelets in a panicle. J Agric Sci, 2004, 142: 177–181



[10]Yang J, Zhang J, Wang Z, Liu K, Wang P. Post-anthesis development of inferior and superior spikelets in rice in relation to abscisic acid and ethylene. J Exp Bot, 2006, 57: 149–160



[11]Nakamura Y, Yuki K, Park S Y. Carbohydrate metabolism in the developing endosperm of rice grains. Plant Cell Physiol, 1989, 30: 833–839



[12]Nakamura Y, Yuki K. Changes in enzyme activities associated with carbohydrate metabolism during development of rice endosperm. Plant Sci, 1992, 82: 15–20



[13]Yang J, Zhang J, Wang Z, Xu G, Zhu Q. Activities of key enzymes in sucrose-to-starch conversion in wheat grains subjected: to water deficit during grain filling. Plant Physiol, 2004, 135:1621–1629



[14]Yang J, Zhang J, Wang Z, Zhu Q, Liu L. Activities of enzymes involved in source-to-starch metabolism in rice grains subjected to water stress during filling. Field Crops Res, 2003, 81: 69–81



[15]Ishimaru T, Hirose T, Matsuda T, Goto A, Takahashi K, Sasaki H, Terao T, Ishii R, Ohsugi R, Yamagishi T. Expression patterns of genes encoding carbohydrate-metabolizing enzymes and their relationship to grain filling in rice (Oryza sativa L.): comparison of caryopses located at different positions in a panicle. Plant Cell Physiol, 2005, 46: 620–628



[16]Huang S-M(黄升谋), Zou Y-B(邹应斌), Liu C-L(刘春林). Setting physiology of the superior and inferior grains of hybrid rice Liangyoupeijiu. Acta Agron Sin (作物学报), 2005, 31(1): 102–107 (in Chinese with English abstract)



[17]Li M-Y(李木英), Pan X-H(潘晓华). The anatomic characters of panicle and its relations to filled grain in two-line hybrid rice. Acta Agric Univ Jiangxiensis (江西农业大学学报), 2000, 22(2): 147–151 (in Chinese with English abstract)



[18]Deng Q-Y(邓启云), Ma G-H(马国辉). A preliminary study on the characters of bundle and its relation to the grain plumpness in intersubspecific hybrid rice. J Hubei Agric Coll (湖北农学院学报), 1992, 2(1): 1–5 (in Chinese with English abstract)



[19]Xiao D-X(肖德兴), Pan X-H(潘晓华), Shi Q-H(石庆华). A preliminary study on the vascular bundle characters and its relation to the filled-grain rate in two lines hybrid rice (F1). Acta Agric Univ Jiangxiensis (江西农业大学学报), 1993, 15(S2): 50–55 (in Chinese with English abstract)



[20]Wang X-Y(王晓燕), Gao R-Q(高荣岐), Dong S-T(董树亭). Study progress in development of endosperm in maize. Chin Agric Sci Bull (中国农学通报), 2005, 21(6): 107–109 (in Chinese with English abstract)



[21]Yang J, Peng S, Visperas R M, Sanico A L, Zhu Q, Gu S. Grain filling pattern and cytokinin content in the grains and roots of rice plants. Plant Growth Regul, 2000, 30: 261–270



[22]Jin D M, Wang W J, Lan S Y. Dynamic status of endogenous IAA, ABA and GA levels in superior and inferior spikelets of heavy panicle hybrid rice during grain filling. J Plant Physiol Mol Biol, 2002, 28: 215–220



[23]Wang Y-F(王艳芳), Cui Z-H(崔震海), Ruan Y-Y (阮燕晔), Ma X-L(马兴林), Guan Y-X(关义新), Zhang L-J(张立军). Changes in endogenous hormone of IAA, GA, ZR and ABA in kernels during grain-filling stage in different types of spring maize (Zea mays L.). Plant Physiol Commun (植物生理学通讯), 2006, 42(2): 225–228 (in Chinese with English abstract)



[24]Zhang H, Tan G, Yang L, Yang J, Zhang J, Zhao B. Hormones in the grains and roots in relation to post-anthesis development of inferior and superior spikelets in japonica/indica hybrid rice. Plant Physiol Bioch, 2009, 47: 195–204



[25]Yang J-C(杨建昌), Qiu M(仇明), Wang Z-Q(王志琴), Liu L-J(刘立军), Zhu Q-S(朱庆森). Relationship between the cell proliferation and cytokinin contents in rice endosperm during its development. Acta Agron Sin (作物学报), 2004, 30(1): 11–17 (in Chinese with English abstract)



[26]Duan J(段俊), Tian C-E(田长恩), Liang C-Y(梁承邺), Huang Y-W(黄毓文), Liu H-X(刘鸿先). Dynamic changes of endogenous plant hormones in rice grains in different parts of panicle at grain-filling stage. Acta Bot Sin (植物学报), 1999, 41(1): 75–79 (in Chinese with English abstract)



[27]Bai X-F(柏新付), Cai Y-P(蔡永萍), Nie F(聂凡). Relationship between abscisic acid and grain filling of rice and wheat. Plant Physiol Commun (植物生理学通讯), 1989, (3): 40–41 (in Chinese with English abstract)



[28]Dai Z-M(戴忠民). Effects of 6-BA and ABA on endosperm cell propagation and starch accumulation in grains of winter wheat. J Triticeae Crops (麦类作物学报), 2008, 28(3): 484–489 (in Chinese with English abstract)



[29]Yang W-B(杨卫兵), Wang Z-L(王振林), Yin Y-P(尹燕枰), Li W-Y(李文阳), Li Y(李勇), Chen X-G(陈晓光), Wang P(王平), Chen E-Y(陈二影), Guo J-X(郭俊祥), Cai T(蔡铁), Ni Y-L(倪英丽). Effects of spraying exogenous ABA or GA on the endogenous hormones concentration and filling of wheat grains. Sci Agric Sin (中国农业科学), 2011, 44(13): 2673–2682 (in Chinese with English abstract)



[30]Diao J-L(刁家连), He Z-P(何钟佩), Hu X-J(胡晓军), Hao L-Y(郝连友), Bi J-H(毕建华), Wang Z-H(王振华). Study of the correlation between endogenous hormones and development of the summer corn ear and kernel. J Chin Agric Univ (中国农业大学学报), 1997, 2(3): 36 (in Chinese with English abstract)



[31]Jiang X-L(姜晓莉), Miao Y-L(苗艳玲), Yang F-T(杨粉团), Li G(李刚). Formation of grain sink of maize (Zea mays L.) and its hormonal regulation. J Jilin Agric Sci (吉林农业科学), 2009, 34(2): 14–16 (in Chinese with English abstract)



[32]Zhang Z-J(张祖建), Zhu Q-S(朱庆森), Wang Z-Q(王志琴). A method of counting endosperm cells in rice grain. J Jiangsu Agric Coll (江苏农学院学报), 1996, 17(2): 7–11 (in Chinese with English abstract)



[33]Zhang Z-J(张祖建), Wang Z-Q(王志琴), Zhu Q-S(朱庆森). Proliferation of endosperm cell and its relation to the growth of grain in rice. Acta Agron Sin (作物学报), 1998, 24(3): 257–264 (in Chinese with English abstract)



[34]Wang X-Y(王晓燕), Dong S-T(董树亭), Gao R-Q(高荣岐), Zhang H-Y(张海艳). Endosperm cell proliferating and its relation to grain weight in different types of maize. Acta Agric Boteali Sin (华北农学报), 2006, 21(2): 23–26 (in Chinese with English abstract)



[35]Zhu Q-S(朱庆森), Cao X-Z(曹显祖), Luo Y-Q(骆亦其). Growth analysis on the process of grain filling in rice. Acta Agron Sin (作物学报), 1988, 14(3): 182–193 (in Chinese with English abstract)



[36]Richards F J. A flexible growth function for empirical use. J Exp Bot, 1959, 10: 290–300



[37]Chen Y-P(陈远平), Yang W-Y(杨文钰). Determination of GA3, IAA, ABA and ZT in dormant buds of Allium ovalifolium by HPLC. J Sichuan Agric Univ (四川农业大学学报), 2005, 23(4): 498–500 (in Chinese with English abstract)



[38]Zhang L(张龙), Yang H-Q(杨洪强), Li Q(李强), Sun X-L(孙晓莉), Qiao H-T(乔海涛). Effects of auxin and cytokinin on PAs content and NO generation of Malus hupehensis Rehd. in vitro. Acta Hortic Sin (园艺学报), 2009, 36(7): 953–958 (in Chinese with English abstract)



[39]Singh G, Gurung S B. Hormonal role in the problem of sterility in Oryza sativa. Plant Physiol Bioch, 1982, 9: 22–32



[40]Wang Z(王忠). Plant Physiology (植物生理学), 2nd edn. Beijing: China Agriculture Press, 2008. pp 335–336 (in Chinese)



[41]Yang J, Zhang J, Wang Z, Xu G, Zhu Q. Activities of key enzymes in sucrose-to-starch conversion in wheat grains subjected to water deficit during grain filling. Plant Physiol, 2004, 135: 1621–1629



[42]Zhou R(周睿), Yang H-Q(杨洪强), Shu H-R(束怀瑞). Regulation of plant sink strength by abscisic acid. Plant Physiol Commun (植物生理学通讯), 1996, 32(3): 223–228 (in Chinese with English abstract)



[43]Van Hengel A J, Barber C, Roberts K. The expression patterns of arabinogalactan-protein AtAGP30 and GLABRA2 reveal a role for abscisic acid in the early stages of root epidermal patterning. Plant J, 2004, 39: 70–83



[44]Brenner M L, Cheikh N. The role of hormones in photosynthate partitioning and seed filling. In: Davies P J ed. Plant Hormones. The Netherlands: Kluwer Academic Publishers, 1995. pp 649–670



[45]Schussler J R, Brenner M L, Brun W A. Relationship of endogenous abscisic acid to sucrose level and seed growth rate of soybeans. Plant Physiol, 1991, 96: 1308–1313



[46]Rook F, Corke F, Card R, Munz G, Smith C, Bevan M W. Impaired sucrose-induction mutants reveal the modulation of sugar-induced starch biosynthetic gene expression by abscisic acid signaling. Plant J, 2001, 26: 421–433



[47]Li X-J(李秀菊), Zhi M-X(职明星), Wei X-Y(卫秀英). Effect of BA and GA on grain weight at different position of winter wheat. Acta Agron Sin (作物学报), 2001, 27(6): 1017–1020 (in Chinese with English abstract)



[48]Chu X-Y(禇孝莹), Wei S(魏湜), Li S-S(李双双), Meng F-M(孟繁美), Fu C(付驰), Sun J(孙继), Gu W-R(顾万荣), Li J(李晶). Effect of exogenous GA3 on grain filling characteristics and yield of triticale. J Triticeae Crops (麦类作物学报), 2012, 32(6): 1156–1160 (in Chinese with English abstract)



[49]Yang J, Zhang J, Wang Z, Zhu Q, Wang W. Hormonal changes in the grains of rice subjected to water stress during grain filling. Plant Physiol, 2001, 127: 315–323



[50]Pharis R P. Gibberellins and reproductive development in seed plants. Annu Rev Plant Physiol, 1985, 36: 517–568



[51]Tan Z-Z(覃章铮), Tang X-H(唐锡华). Studies on the developmental biology of embryogenesis in higherplants: VI. Dynamic changes in some of themacromolecules during embryogenesis of Oryza sativa L. subsp. japonica. Acta Phytophysiol Sin (植物生理学报), 1982, 8(3): 295–305 (in Chinese with English abstract)



[52]Wang J-H(王纪华), Wang S-A(王树安), Zhao D-M(赵冬梅), Ni J-C(倪军昌). Study on regulation of development of maize kernels: III. The approach to chemical regulation under conditions of tissue culture. Acta Agron Sin (作物学报), 1996, 22(2): 208–213 (in Chinese with English abstract)

[1] 刘恩波, 陈静, 李红星, 于宁宁, 任佰朝, 赵斌, 刘鹏, 张吉旺. 遮阴改变源-库平衡和调节碳水化合物代谢进而抑制夏玉米幼穗发育[J]. 作物学报, 2026, 52(6): 1891-1901.
[2] 梁进宇, 尹嘉德, 王红丽, 张国平, 侯慧芝, 董博, 马明生. 基于无人机高光谱和机器学习的旱地饲用玉米叶片氮含量估测[J]. 作物学报, 2026, 52(6): 1788-1801.
[3] 金昱何, 王雪菲, 徐张一娃, 缪怡宁, 蒋云杰, 伊莹, 缪德麟, 朱静仪, 钟一帆, 陈铭亨, 方芳, 刘鹏. 外源激素对低温胁迫下大豆叶片叶绿素荧光参数及抗氧化酶系统的影响[J]. 作物学报, 2026, 52(6): 1817-1829.
[4] 孙淑凤, 许振南, 黄嘉鑫, 翁建峰, 李新海. 玉米MAPK家族全基因组鉴定及其对拟轮枝镰孢菌感染的响应[J]. 作物学报, 2026, 52(5): 1291-1308.
[5] 张宁宁, 滕雨菲, 任娜娜, 魏兴卓, 闫书豪, 樊可心, 王永宏, 陈文康, 张兴华, 朱万超, 徐淑兔, 薛吉全. 201份玉米自交系抗旱表型评价及可塑性分析[J]. 作物学报, 2026, 52(5): 1309-1325.
[6] 杨扬, 常诗惠, 田红丽, 易红梅, 王璐, 任洁, 范亚明, 刘亚维, 王凤格, 赵久然. 不同生态区国审玉米品种的遗传多样性分析[J]. 作物学报, 2026, 52(5): 1352-1364.
[7] 蔡兆琴, 何观咏, 何文, 阮丽霞, 梁振华, 李永珍, 李恒锐, 陈会鲜. 木薯分枝发育过程的动态转录组分析与关键基因发掘[J]. 作物学报, 2026, 52(5): 1430-1441.
[8] 杨欣雨, 崔文涛, 迪力尼格尔·阿力木, 汪凯翔, 吴鹏昊, 任姣姣. 玉米穗上叶片数全基因组关联分析和全基因组选择[J]. 作物学报, 2026, 52(5): 1573-1590.
[9] 韩亚鑫, 何冠华, 张小琼, 张登峰, 李永祥, 刘旭洋, 王天宇, 黎裕, 邹华文, 李春辉. 基于RNA-Seq和BSA-Seq联合分析挖掘玉米侧根密度基因资源[J]. 作物学报, 2026, 52(5): 1341-1352.
[10] 张振, 冯连杰, 石玉, 于振文, 张永丽. 节水补灌下不同穗型小麦产量形成差异研究[J]. 作物学报, 2026, 52(5): 1522-1535.
[11] 刘昕萌, 任昊, 张继波, 张吉旺, 赵斌, 任佰朝, 刘鹏, 王洪章. 茉莉酸甲酯(MeJA)缓解高温影响玉米雌穗分化的生理机制[J]. 作物学报, 2026, 52(5): 1561-1572.
[12] 张鸿蓉, 王菲儿, 李盼, 仇海龙, 朱静, 赵连豪, 南运有, 何蔚, 樊志龙, 胡发龙, 柴强, 殷文. 减量20%灌水与25%有机肥替代化肥提高青贮玉米产量的光合特性[J]. 作物学报, 2026, 52(5): 1487-1500.
[13] 尚云秋, 赵竹, 陈欢, 丁永刚, 乔玉强, 李玮, 张向前, 曹承富, 杜世州. 长期定位耕作方式对雨养小麦籽粒灌浆和产量形成的影响[J]. 作物学报, 2026, 52(4): 1236-1250.
[14] 蔡宏玮, 于爱忠, 姜科强, 王鹏飞, 王玉珑, 霍建喆, 庞小能, 尹波, 尚永盼. 干旱灌区有机肥替代部分化肥促进甜玉米产量提升的关键机制[J]. 作物学报, 2026, 52(4): 1166-1180.
[15] 田红丽, 杨扬, 范亚明, 易红梅, 郭丹丹, 王凤格, 赵久然. 适于玉米品种鉴定的一套三等位变异SNP新型标记组合[J]. 作物学报, 2026, 52(4): 993-1005.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!