作物学报 ›› 2013, Vol. 39 ›› Issue (08): 1452-1461.doi: 10.3724/SP.J.1006.2013.01452
徐云姬,顾道健,张博博,张耗,王志琴,杨建昌*
XU Yun-Ji,GU Dao-Jian,ZHANG Bo-Bo,ZHANG Hao,WANG Zhi-Qin,YANG Jian-Chang*
摘要:
以玉米品种登海11为材料, 分别进行大田和温室试验, 观察灌浆期果穗不同部位籽粒玉米素(Z)+玉米素核苷(ZR)、吲哚-3-乙酸(IAA)、脱落酸(ABA)和赤霉素(GA3)含量变化及其与胚乳发育和籽粒灌浆的关系。结果显示, 籽粒最大胚乳细胞数目、最大胚乳细胞增殖速率及平均速率、最大灌浆速率、平均灌浆速率和百粒重表现为果穗下部籽粒>中部籽粒>上部籽粒。在胚乳细胞活跃增殖期或活跃灌浆期, 籽粒Z+ZR、IAA和ABA含量以果穗下部籽粒最高, 中部籽粒其次, 上部籽粒最低。GA3含量则为果穗上部籽粒>中部籽粒>下部籽粒。两个试验的结果趋势一致。胚乳细胞增殖速率和籽粒灌浆速率与籽粒Z+ZR、IAA和ABA含量呈极显著正相关, 与籽粒GA3含量呈显著负相关。说明玉米果穗上部籽粒轻主要是由于这些籽粒的胚乳细胞增殖速率小, 导致其胚乳细胞数少, 这与其灌浆期较低的Z+ZR、IAA和ABA含量及较高的GA3含量有密切关系。
[1]State Administration of Grain, P. R. China (国家粮食局). http://nongye.cntv.cn/20121017/102023.shtml[2]Dong M-H(董明辉), Zhao B-H(赵步洪), Wu X-Z(吴翔宙), Chen T(陈涛), Yang J-C(杨建昌). Difference in hormonal content and activities of key enzymes in the grains at different positions on a rice panicle during grain filling and their correlations with rice qualities. Sci Agric Sin (中国农业科学), 2008, 41(2): 370–380 (in Chinese with English abstract)[3]Ren Y-M(任亚梅), Liu X-H(刘兴华), Yuan C-L(袁春龙), Zheng J-M(郑建梅), Luo A-W(罗安伟). Study on the carbohydrate change at different parts of fresh unripe maize ears. J Food Sci Biotechnol (食品与生物技术学报), 2005, 24(5): 66–70 (in Chinese with English abstract)[4]Tang Q-F(唐祈福), Rong T-Z(荣廷昭). The relationship between endogenous hormone and barren ear tip of maize. J Nucl Agric Sci (核农学报), 2007, 21(4): 366–368 (in Chinese with English abstract)[5]Sikder H P, Gupta D K D. Physiology of grain in rice. Indian Agric, 1976, 20: 133–141[6]Wang Y. Effectiveness of supplied nitrogen at the primordial panicle stage on rice characteristics and yields. Int Rice Res Newslettt, 1981, 6: 23–24[7]Murty P S S, Murty K S. Spikelet sterility in relation to nitrogen and carbohydrate contents in rice. Indian J Plant Physiol, 1982, 25: 40–48[8]Mohapatra P K, Patel R, Sahu S K. Time of flowering affects grain quality and spikelet partitioning within the rice panicle. Aust J Plant Physiol, 1993, 20: 231–242[9]Kato T. Effect of spikelet removal on the grain filling of Akenohoshi, a rice cultivar with numerous spikelets in a panicle. J Agric Sci, 2004, 142: 177–181[10]Yang J, Zhang J, Wang Z, Liu K, Wang P. Post-anthesis development of inferior and superior spikelets in rice in relation to abscisic acid and ethylene. J Exp Bot, 2006, 57: 149–160[11]Nakamura Y, Yuki K, Park S Y. Carbohydrate metabolism in the developing endosperm of rice grains. Plant Cell Physiol, 1989, 30: 833–839[12]Nakamura Y, Yuki K. Changes in enzyme activities associated with carbohydrate metabolism during development of rice endosperm. Plant Sci, 1992, 82: 15–20[13]Yang J, Zhang J, Wang Z, Xu G, Zhu Q. Activities of key enzymes in sucrose-to-starch conversion in wheat grains subjected: to water deficit during grain filling. Plant Physiol, 2004, 135:1621–1629[14]Yang J, Zhang J, Wang Z, Zhu Q, Liu L. Activities of enzymes involved in source-to-starch metabolism in rice grains subjected to water stress during filling. Field Crops Res, 2003, 81: 69–81[15]Ishimaru T, Hirose T, Matsuda T, Goto A, Takahashi K, Sasaki H, Terao T, Ishii R, Ohsugi R, Yamagishi T. Expression patterns of genes encoding carbohydrate-metabolizing enzymes and their relationship to grain filling in rice (Oryza sativa L.): comparison of caryopses located at different positions in a panicle. Plant Cell Physiol, 2005, 46: 620–628[16]Huang S-M(黄升谋), Zou Y-B(邹应斌), Liu C-L(刘春林). Setting physiology of the superior and inferior grains of hybrid rice Liangyoupeijiu. Acta Agron Sin (作物学报), 2005, 31(1): 102–107 (in Chinese with English abstract)[17]Li M-Y(李木英), Pan X-H(潘晓华). The anatomic characters of panicle and its relations to filled grain in two-line hybrid rice. Acta Agric Univ Jiangxiensis (江西农业大学学报), 2000, 22(2): 147–151 (in Chinese with English abstract)[18]Deng Q-Y(邓启云), Ma G-H(马国辉). A preliminary study on the characters of bundle and its relation to the grain plumpness in intersubspecific hybrid rice. J Hubei Agric Coll (湖北农学院学报), 1992, 2(1): 1–5 (in Chinese with English abstract)[19]Xiao D-X(肖德兴), Pan X-H(潘晓华), Shi Q-H(石庆华). A preliminary study on the vascular bundle characters and its relation to the filled-grain rate in two lines hybrid rice (F1). Acta Agric Univ Jiangxiensis (江西农业大学学报), 1993, 15(S2): 50–55 (in Chinese with English abstract)[20]Wang X-Y(王晓燕), Gao R-Q(高荣岐), Dong S-T(董树亭). Study progress in development of endosperm in maize. Chin Agric Sci Bull (中国农学通报), 2005, 21(6): 107–109 (in Chinese with English abstract)[21]Yang J, Peng S, Visperas R M, Sanico A L, Zhu Q, Gu S. Grain filling pattern and cytokinin content in the grains and roots of rice plants. Plant Growth Regul, 2000, 30: 261–270[22]Jin D M, Wang W J, Lan S Y. Dynamic status of endogenous IAA, ABA and GA levels in superior and inferior spikelets of heavy panicle hybrid rice during grain filling. J Plant Physiol Mol Biol, 2002, 28: 215–220[23]Wang Y-F(王艳芳), Cui Z-H(崔震海), Ruan Y-Y (阮燕晔), Ma X-L(马兴林), Guan Y-X(关义新), Zhang L-J(张立军). Changes in endogenous hormone of IAA, GA, ZR and ABA in kernels during grain-filling stage in different types of spring maize (Zea mays L.). Plant Physiol Commun (植物生理学通讯), 2006, 42(2): 225–228 (in Chinese with English abstract)[24]Zhang H, Tan G, Yang L, Yang J, Zhang J, Zhao B. Hormones in the grains and roots in relation to post-anthesis development of inferior and superior spikelets in japonica/indica hybrid rice. Plant Physiol Bioch, 2009, 47: 195–204[25]Yang J-C(杨建昌), Qiu M(仇明), Wang Z-Q(王志琴), Liu L-J(刘立军), Zhu Q-S(朱庆森). Relationship between the cell proliferation and cytokinin contents in rice endosperm during its development. Acta Agron Sin (作物学报), 2004, 30(1): 11–17 (in Chinese with English abstract)[26]Duan J(段俊), Tian C-E(田长恩), Liang C-Y(梁承邺), Huang Y-W(黄毓文), Liu H-X(刘鸿先). Dynamic changes of endogenous plant hormones in rice grains in different parts of panicle at grain-filling stage. Acta Bot Sin (植物学报), 1999, 41(1): 75–79 (in Chinese with English abstract)[27]Bai X-F(柏新付), Cai Y-P(蔡永萍), Nie F(聂凡). Relationship between abscisic acid and grain filling of rice and wheat. Plant Physiol Commun (植物生理学通讯), 1989, (3): 40–41 (in Chinese with English abstract)[28]Dai Z-M(戴忠民). Effects of 6-BA and ABA on endosperm cell propagation and starch accumulation in grains of winter wheat. J Triticeae Crops (麦类作物学报), 2008, 28(3): 484–489 (in Chinese with English abstract)[29]Yang W-B(杨卫兵), Wang Z-L(王振林), Yin Y-P(尹燕枰), Li W-Y(李文阳), Li Y(李勇), Chen X-G(陈晓光), Wang P(王平), Chen E-Y(陈二影), Guo J-X(郭俊祥), Cai T(蔡铁), Ni Y-L(倪英丽). Effects of spraying exogenous ABA or GA on the endogenous hormones concentration and filling of wheat grains. Sci Agric Sin (中国农业科学), 2011, 44(13): 2673–2682 (in Chinese with English abstract)[30]Diao J-L(刁家连), He Z-P(何钟佩), Hu X-J(胡晓军), Hao L-Y(郝连友), Bi J-H(毕建华), Wang Z-H(王振华). Study of the correlation between endogenous hormones and development of the summer corn ear and kernel. J Chin Agric Univ (中国农业大学学报), 1997, 2(3): 36 (in Chinese with English abstract)[31]Jiang X-L(姜晓莉), Miao Y-L(苗艳玲), Yang F-T(杨粉团), Li G(李刚). Formation of grain sink of maize (Zea mays L.) and its hormonal regulation. J Jilin Agric Sci (吉林农业科学), 2009, 34(2): 14–16 (in Chinese with English abstract)[32]Zhang Z-J(张祖建), Zhu Q-S(朱庆森), Wang Z-Q(王志琴). A method of counting endosperm cells in rice grain. J Jiangsu Agric Coll (江苏农学院学报), 1996, 17(2): 7–11 (in Chinese with English abstract)[33]Zhang Z-J(张祖建), Wang Z-Q(王志琴), Zhu Q-S(朱庆森). Proliferation of endosperm cell and its relation to the growth of grain in rice. Acta Agron Sin (作物学报), 1998, 24(3): 257–264 (in Chinese with English abstract)[34]Wang X-Y(王晓燕), Dong S-T(董树亭), Gao R-Q(高荣岐), Zhang H-Y(张海艳). Endosperm cell proliferating and its relation to grain weight in different types of maize. Acta Agric Boteali Sin (华北农学报), 2006, 21(2): 23–26 (in Chinese with English abstract)[35]Zhu Q-S(朱庆森), Cao X-Z(曹显祖), Luo Y-Q(骆亦其). Growth analysis on the process of grain filling in rice. Acta Agron Sin (作物学报), 1988, 14(3): 182–193 (in Chinese with English abstract)[36]Richards F J. A flexible growth function for empirical use. J Exp Bot, 1959, 10: 290–300[37]Chen Y-P(陈远平), Yang W-Y(杨文钰). Determination of GA3, IAA, ABA and ZT in dormant buds of Allium ovalifolium by HPLC. J Sichuan Agric Univ (四川农业大学学报), 2005, 23(4): 498–500 (in Chinese with English abstract)[38]Zhang L(张龙), Yang H-Q(杨洪强), Li Q(李强), Sun X-L(孙晓莉), Qiao H-T(乔海涛). Effects of auxin and cytokinin on PAs content and NO generation of Malus hupehensis Rehd. in vitro. Acta Hortic Sin (园艺学报), 2009, 36(7): 953–958 (in Chinese with English abstract)[39]Singh G, Gurung S B. Hormonal role in the problem of sterility in Oryza sativa. Plant Physiol Bioch, 1982, 9: 22–32[40]Wang Z(王忠). Plant Physiology (植物生理学), 2nd edn. Beijing: China Agriculture Press, 2008. pp 335–336 (in Chinese)[41]Yang J, Zhang J, Wang Z, Xu G, Zhu Q. Activities of key enzymes in sucrose-to-starch conversion in wheat grains subjected to water deficit during grain filling. Plant Physiol, 2004, 135: 1621–1629[42]Zhou R(周睿), Yang H-Q(杨洪强), Shu H-R(束怀瑞). Regulation of plant sink strength by abscisic acid. Plant Physiol Commun (植物生理学通讯), 1996, 32(3): 223–228 (in Chinese with English abstract)[43]Van Hengel A J, Barber C, Roberts K. The expression patterns of arabinogalactan-protein AtAGP30 and GLABRA2 reveal a role for abscisic acid in the early stages of root epidermal patterning. Plant J, 2004, 39: 70–83[44]Brenner M L, Cheikh N. The role of hormones in photosynthate partitioning and seed filling. In: Davies P J ed. Plant Hormones. The Netherlands: Kluwer Academic Publishers, 1995. pp 649–670[45]Schussler J R, Brenner M L, Brun W A. Relationship of endogenous abscisic acid to sucrose level and seed growth rate of soybeans. Plant Physiol, 1991, 96: 1308–1313[46]Rook F, Corke F, Card R, Munz G, Smith C, Bevan M W. Impaired sucrose-induction mutants reveal the modulation of sugar-induced starch biosynthetic gene expression by abscisic acid signaling. Plant J, 2001, 26: 421–433[47]Li X-J(李秀菊), Zhi M-X(职明星), Wei X-Y(卫秀英). Effect of BA and GA on grain weight at different position of winter wheat. Acta Agron Sin (作物学报), 2001, 27(6): 1017–1020 (in Chinese with English abstract)[48]Chu X-Y(禇孝莹), Wei S(魏湜), Li S-S(李双双), Meng F-M(孟繁美), Fu C(付驰), Sun J(孙继), Gu W-R(顾万荣), Li J(李晶). Effect of exogenous GA3 on grain filling characteristics and yield of triticale. J Triticeae Crops (麦类作物学报), 2012, 32(6): 1156–1160 (in Chinese with English abstract)[49]Yang J, Zhang J, Wang Z, Zhu Q, Wang W. Hormonal changes in the grains of rice subjected to water stress during grain filling. Plant Physiol, 2001, 127: 315–323[50]Pharis R P. Gibberellins and reproductive development in seed plants. Annu Rev Plant Physiol, 1985, 36: 517–568[51]Tan Z-Z(覃章铮), Tang X-H(唐锡华). Studies on the developmental biology of embryogenesis in higherplants: VI. Dynamic changes in some of themacromolecules during embryogenesis of Oryza sativa L. subsp. japonica. Acta Phytophysiol Sin (植物生理学报), 1982, 8(3): 295–305 (in Chinese with English abstract)[52]Wang J-H(王纪华), Wang S-A(王树安), Zhao D-M(赵冬梅), Ni J-C(倪军昌). Study on regulation of development of maize kernels: III. The approach to chemical regulation under conditions of tissue culture. Acta Agron Sin (作物学报), 1996, 22(2): 208–213 (in Chinese with English abstract) |
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