欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2014, Vol. 40 ›› Issue (02): 369-379.doi: 10.3724/SP.J.1006.2014.00369

• 研究简报 • 上一篇    

新疆自育陆地棉品种SSR遗传多样性分析

艾先涛1,2,梁亚军2,沙红2,王俊铎2,郑巨云2,吐尔逊江2,多力坤2,李雪源2,*,华金平3,*   

  1. 1新疆农业大学, 乌鲁木齐 830052；2新疆农业科学院经济作物研究所, 乌鲁木齐 830091；3中国农业大学植物遗传育种系 / 杂种优势研究与利用教育部重点实验室 / 作物遗传改良北京市重点实验室, 北京 100193
  • 收稿日期:2013-01-21 修回日期:2013-08-16 出版日期:2014-02-12 网络出版日期:2013-10-22
  • 通讯作者: 华金平, E-mail: jinping_hua@cau.edu.cn; 李雪源, E-mail: xjmh2338@163.com
  • 基金资助:

    本研究由新疆自然科学基金项目(2012211B47)和国家高技术研究发展计划(863计划)项目(2012AA101108)资助。

Genetic Diversity Analysis on Local Upland Cotton Cultivars in Xinjiang Based on SSR Markers

AI Xian-Tao1, 2,LIANG Ya-Jun2,SHA Hong2,WANG Jun-Duo2,ZHENG Ju-Yun2,Tu-Er-Xun-Jiang2,DUO Li-Kun2,LI Xue-Yuan2, *,HUA Jin-Ping3,*   

  1. 1Xinjiang Agricultural University , Urumqi 830052, China; 2 Economic Crop Research Institute, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China; 3 Department of Plant Genetics and Breeding / Key Laboratory of Crop Heterosis and Utilization of Ministry of Education / Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
  • Received:2013-01-21 Revised:2013-08-16 Published:2014-02-12 Published online:2013-10-22
  • Contact: 华金平, E-mail: jinping_hua@cau.edu.cn; 李雪源, E-mail: xjmh2338@163.com

RichHTML

PDF

1379

被引次数

32

摘要：

利用SSR标记对94份新疆自育陆地棉品种的基因组进行分析，研究新疆陆地棉品种的遗传多样性。结果: 从分布于棉花全基因组的206对SSR标记中筛选出54对具有稳定多态性的引物, 共检测出153个多态性位点, 每对引物的等位变异为2~6个，平均为2.93个；基因型多样性(H′)变幅为0.0439–0.7149，平均为0.4491；引物多态信息含量(PIC)为0.0430–0.6640，平均为0.3831。表明SSR标记在品种间可以反映较丰富的遗传多样性信息。94份品种间成对遗传相似系数变幅为0.3846~0.9835, 71.9%的品种相似系数在0.601~0.800内，反映出新疆陆地棉品种间的遗传相似性相对较高。根据UPGMA 聚类分析，在阈值为0.63时，将94份品种划分为2个类群，说明新疆陆地棉品种间遗传关系相对简单，品种的遗传基础相对狭窄，品种遗传组分差异较小，总体上遗传多样性不够丰富；分子聚类结果与品种本身遗传系谱背景和演变趋势吻合度较高，符合品种的真实特性。研究证明，自育品种在分子水平上差异不大，需要努力拓宽品种选育的遗传基础。

关键词: 陆地棉, 遗传背景, SSR, 遗传多样性, 亲缘关系

Abstract:

Xinjiang, the largest prior cotton production region in China, has made remarkable genetic improvement in upland cotton (Gossypium hirsutum L.). There has been a substantial raise in yield potential, cotton-planting area and total production in Xinjiang since 1970s. The new upland cotton cultivars have contributed a lot to yield increase, in which Xinjiang-bred upland cotton varieties play an important role in cotton production in both Northern and Southern Xinjiang. However, seldom research on genetic basis of genetic diversity and pedigree relationship has been evaluated in upland cotton varieties in Xinjiang so far. This study aimed to reveal the genetic diversity of 94 upland cotton varieties in Xinjiang by genome analysis using SSR markers. The results indicated that SSR molecular markers could be employed in revealing the genetic diversity. One hundred and fifty-three polymorphic lociwere detected by amplified with 54 pairs of primers with allelic variation of 2-6 per primer pair and the average of 2.93. The genotypic diversity (H’) spanned from 0.0439 to 0.7149, with the average of 0.4491. The PIC value ranged from 0.0430 to 0.6640, and the average was 0.3831. The coefficients of genetic similarity among 94 tested cultivars varied from 0.3846 to 0.9835. And more than 71.9% of similarity coefficients fell into the interval of 0.601–0.800, indicating that the genetic similarity among the cultivars was high, whereas the genetic diversity might not be abundant. According to UPGMA cluster analysis, 94 tested cultivars were divided into two clusters when the threshold valve was given as 0.63, showing the relatively simple genetic relationship among the upland cotton varieties in Xinjiang, and relatively low genetic diversity and narrow genetic basis. The results of molecular clustering on genetic diversity of upland cotton coincided in large degree with the pedigree relationship. In conclusion, there is no much difference on molecular level among Xinjiang-bred upland cotton varieties. Materials with much wider genetic backgrounds are suggested to be used to diversify the genetic basis of cotton varieties in Xinjiang.

Key words: Upland cotton, Genetic background, SSR, Genetic diversity, Genetic relationship

[1]周盛汉. 中国棉花品种系谱图. 成都: 四川科学技术出版社, 2000



Zhou S H. China Cotton Varieties Pedigree Diagram. Sichuan Publishing House of Science & Technology, 2000 (in Chinese)



[2]董玉琛, 刘旭. 中国作物野生近缘植物及其保护. 见: 中国科学院生物多样性委员会等编. 生物多样性与人类未来. 北京: 中国林业出版社, 1998, pp 24–29



Dong Y C, Liu X. Wild relative crops and their protection in China. In: Biodiversity Committee, CAS, etc. eds. Biological Diversity and the Future of Human Beings, Beijing: Chinese Forestry Press 1998, pp 24–29 (in Chinese)



[3]朱四元, 陈金湘, 刘爱玉, 李瑞莲, 严跃文, 唐海明. 利用SSR标记对不同类型抗虫棉品种的遗传多样性分析. 湖南农业大学学报, 2006, 32: 469–472



Zhu S Y, Chen J X, Liu A Y, Li R L, Yan Y W, Tang H M. Genetic diversity analysis and identification of insect-resistant cottons based upon SSR markers. J Hunan Agric Univ (Nat Sci), 2006, 32: 469–472 (in Chinese with English abstract)



[4]武耀廷, 张天真, 殷剑美. 利用分子标记和形态学性状检测的陆地棉栽培品种遗传多样性. 遗传学报, 2001, 28: 1040–1050



Wu Y T, Zhang T Z, Yin J M. Genetic diversity detected by DNA markers and phenotypes in upland cotton. Acta Genet Sin, 2001, 28: 1040–1050 (in Chinese with English abstract)



[5]李武, 倪薇, 林忠旭, 张献龙. 海岛棉遗传多样性的SRAP标记分析. 作物学报, 2008, 34: 893–898



Li W, Ni W, Lin Z X, Zhang X L. Genetic diversity analysis of sea-island cotton cultivars using SRAP markers. Acta Agron Sin, 2008, 34(5): 893–898 (in Chinese with English abstract)



[6]郭旺珍,周兆华,张天真. RAPD鉴定棉花抗(耐)黄萎病品种(系)的遗传变异研究. 江苏农业学报,1999, 15(1): 1–6



Guo W Z, Zhou Z H, Zhang T Z. A study on cotton genetic variation of Verticillium wilt resistance based on RAPDs. J Jiangsu Agric, 1999, 15(1): 1–6 (in Chinese with English abstract)



[7]凌磊, 李廷春, 李正鹏. 利用SRAP标记分析彩色棉与白色棉的遗传差异. 中国农学通报, 2009, 25(16): 32–38



Ling L, Li T C, Li Z P. Genetic difference analysis between colored cotton and white cotton by SRAP markers. Chin Agric Sci Bull, 2009, 25(16): 32–38 (in Chinese with English abstract)



[8]卫泽, 孙学振, 宋宪亮. 国内外57份棉花种质资源的遗传多样性研究. 山东农业科学, 2010, 6: 13–18



Wei Z, Sun X Z, Song X L. Genetic diversity of 57 cotton germplasm resources from China and overseas. Shandong Agric Sci, 2010, (6): 13–18 (in Chinese with English abstract)



[9]徐秋华, 张献龙, 聂以春. 长江、黄河两棉区陆地棉品种遗传多样性比较研究. 遗传学报, 2001, 8: 683–690



Xu Q H, Zhang X L, Nie Y C. Genetic diversity evaluation of cultivars (L.) from the Chang Jiang River Valley and the Huanghe Valley by RAPD. Acta Genet Sin, 2001, 28: 683–690 (in Chinese with English abstract)



[10]刘文欣, 孔繁玲, 郭志丽, 张群远, 彭慧茹, 付小琼, 杨付新. 建国以来我国棉花品种遗传基础的分子标记分析. 遗传学报, 2003, 30: 560–570



Liu W X, Kong F L, Guo Z L, Zhang Q Y, Peng H R, Fu X Q, Yang F X. Analysis about genetic basis of cotton cultivars in China since 1949 with molecular markers. Acta Genet Sin, 2003, 30: 560–570 (in Chinese with English abstract)



[11]宋国立, 崔荣霞, 王坤波, 郭立平, 黎绍惠, 王春英, 张香娣. 改良CTAB法快速提取棉花DNA. 棉花学报, 1998, 10(5): 273–275



Song G L, Cui R X, Wang K B, Guo L P, Li S H, Wang C Y, Zhang X D. The improved CTAB law rapid extraction of genomic DNA from cotton. Acta Gossypii Sin, 1998, 10: 273–275 (in Chinese with English abstract)



[12]Xiao J, Wu K, Fang D D, Stelly D M, Yu J, Cantrell R G. New SSR markers for Use in cotton (Gossypium spp.) improvement. J Cotton Sci, 2009, 13: 75–157



[13]艾先涛, 李雪源, 秦文斌, 孙国清, 吐尔逊江, 莫明. 新疆陆地棉育种遗传组分拓展研究. 分子植物育种, 2005, 3: 575–578



Ai X T, Li X Y, Qin W B, Sun G Q, Tu E X J, Mo M. The extend study on genetic composition of upland cotton breeding in Xinjiang. Mol Plant Breed, 2005, 3: 575–578 (in Chinese with English abstract)



[14]贺道华, 邢宏宜, 李婷婷, 汤益, 曾舟. 92份棉花资源遗传多样性的SSR分析. 西北植物学报, 2010, 30: 1557–1564



He D H, Xing H Y, Li T T, Tang Y, Zeng Z. Genetic diversity of 92 cotton accessions evaluated with SSR markers. Northwest Agric Univ, 2010, 30: 1557–1564 (in Chinese with English abstract)



[15]高伟, 刘方, 黎绍惠, 王春英, 张香娣, 王玉红, 王坤波. 利用SSR分析四倍体棉种多态性.作物学报, 2010, 36: 1902–1909



Gao W, Liu F, Li S H, Wang C Y, Zhang X D, Wang Y H, Wang K B. Genetic diversity of allotetraploid cotton based on SSR markers. Acta Agron Sin, 2010, 36: 1902−1909 (in Chinese with English abstract)



[16]别墅, 孔繁玲, 周有耀, 张光梅, 张群远, 王孝纲. 中国三大主产棉区棉花品种遗传多样性的RAPD及其与农艺性状关系的研究. 中国农业科学, 2001, 34: 597–603



Bie S, Kong F L, Zhou Y Y, Zhang G M, Zhang Q Y, Wang X G. Genetic diversity analysis representative elite cotton varieties in three main cotton regions in China by RAPD and its relation with agronomic characteristics. Sci Agric Sin, 2001, 34: 597–603 (in Chinese with English abstract)



[17]陈光. 我国陆地棉基础种质及其衍生品种的遗传多样性. 中国农业科学院研究生院硕士学位论文, 2005



Chen G. Genetic Diversity of Basic Germplasm and Their Offsprings of Upland Cotton in China. MS Thesis of Graduate School of Chinese Academy of Agricultural Sciences, 2005 (in Chinese with English abstract)



[18]杜雄明, 刘国强. 论我国棉花育种的基础种质. 植物遗传资源学报. 2004, 5: 69–74



Du X M, Liu G Q. Basic Germplasm for Cotton Breeding in China. J Plant Genet Resour, 2004, 5: 69–74 (in Chinese with English abstract)



[19]薛艳, 张新宇, 沙红, 李雪源, 孙杰, 李保成. 新疆早熟棉品种SSR指纹图谱构建与品种鉴别. 棉花学报, 2010, 22: 360–366



Xue Y, Zhang X Y, Sha H, Li X Y, Sun J, Li B C. Construction of Fingerprinting Map Based on SSR and Identification of Cultivars for Earliness Cultivars in Upland Cotton in Xinjiang. Acta Gossypii Sin, 2010, 22: 360–366 (in Chinese with English abstract)



[20]黄滋康, 季道藩. 中国棉花遗传育种学. 济南: 山东科学技术出版社, 2003



Hang Z K, Ji D F. China Cotton Genetics and Breeding. Jinan: Shandong Science & Technology Press, 2003



[21]孙杰, 褚贵新, 张文辉, 金文虹. 新疆特早熟棉区棉花品种主要性状演变趋势研究. 中国棉花, 1999, 26(7): 14–16



Sun J, Zhe G X, Zhang W H, Jin W H. The main character trend of especially early maturing varieties of cotton in Xinjiang. China’s Cotton, 1999, 26(7): 14–16 (in Chinese with English abstract)



[22]黄滋康. 中国棉花品种及其系谱. 北京: 中国农业出版社, 2007



Hang Z K. China's cotton varieties and its pedigree. Beijing: China Agricultural Press, 2007



[23]Yu F F, Huaxia Y F, Lu W J, Wu C G, Cao X C, Guo X Q. GhWRKY15, a member of the WRKY transcription factor family identified from cotton (Gossypium hirsutum L.), is involved in disease resistance and plant development, BMC Plant Biol, 2012, 12: 144



[24]Zhang Y, Wang X F, Li Z K, Zhang G Y. Assessing genetic diversity of cotton cultivars using genomic and newly developed expressed sequence tag-derived microsatellite markers. Genet Mol Res, 2011, 10: 1462–1470
[1] 彭佳泺, 李颖, 李丹丹, 杨军宁, 郭学峰, 张文姣, 俞晓雪, 周亚荣, 王振玉, 王彩香, 马雄风, 宿俊吉. 陆地棉I类LBD家族成员鉴定及GhLBD6调控开花期的功能和单倍型分析[J]. 作物学报, 2026, 52(6): 1682-1697.
[2] 张颖星, 宋裕祯, 王跃, 曹越, 曹晓宁, 王瑞云. EMS诱导糜子优异性状突变体的筛选及表型分析[J]. 作物学报, 2026, 52(5): 1388-1400.
[3] 杨扬, 常诗惠, 田红丽, 易红梅, 王璐, 任洁, 范亚明, 刘亚维, 王凤格, 赵久然. 不同生态区国审玉米品种的遗传多样性分析[J]. 作物学报, 2026, 52(5): 1352-1364.
[4] 徐建霞, 丁延庆, 曹宁, 程斌, 高旭, 李文贞, 王若若, 王磊, 张立异. 397份高粱种质资源在贵州表型多样性分析及综合评价[J]. 作物学报, 2026, 52(4): 1073-1087.
[5] 田春艳, 陆鑫, 吴才文, 徐超华, 刘家勇, 边芯, 桃联安. 基于荧光SSR的甘蔗创新种质遗传多样性分析及育种潜力评估[J]. 作物学报, 2026, 52(4): 1057-1072.
[6] 侯洁, 付朵朵, 武海峰, 郝宇琼, 郑兴卫, 武棒棒, 周凯, 李晓华, 郑军, 赵佳佳. 山西省小麦地方品种的染色体多样性及遗传效应分析[J]. 作物学报, 2026, 52(3): 746-763.
[7] 梅飘, 刘丁丁, 叶圆圆, 张晨禹, 丁诗琦, 李亚奇, 王培鑫, 梅菊芬, 马春雷. 基于茶树液相功能芯片的白化茶树资源遗传多样性分析[J]. 作物学报, 2025, 51(9): 2358-2370.
[8] 李宜谦, 徐守振, 刘萍, 马麒, 谢斌, 陈红. 基于40K SNP芯片的陆地棉产量构成因素全基因组关联分析及单铃重位点挖掘[J]. 作物学报, 2025, 51(8): 2128-2138.
[9] 薛晓菲, 戴云静, 李熙林, 丁艳艳, 王翔, 雷长英, 韩焕勇, 贺道华. 陆地棉杜松烯合酶基因GhCDN10的特征及其在棉酚合成中功能分析[J]. 作物学报, 2025, 51(8): 2060-2076.
[10] 王天译, 杨绣娟, 赵佳佳, 郝宇琼, 郑兴卫, 武棒棒, 李晓华, 郝水源, 郑军. 山西小麦醇溶蛋白多样性及其对面粉品质效应研究[J]. 作物学报, 2025, 51(7): 1784-1800.
[11] 郭淑慧, 潘转霞, 赵战胜, 杨六六, 皇甫张龙, 郭宝生, 胡晓丽, 录亚丹, 丁霄, 吴翠翠, 兰刚, 吕贝贝, 谭逢平, 李朋波. 陆地棉D11染色体一个纤维长度主效位点的遗传解析[J]. 作物学报, 2025, 51(2): 383-394.
[12] 王浩辰, 王克晶, 韩娟, 李向华. 东南沿海短绒野大豆两种代表性生境自然种群的空间遗传结构特征:种群内取样策略研究[J]. 作物学报, 2025, 51(11): 2875-2885.
[13] 赵海红, 李梦媛, 刘锦婧, 王园园, 杜磊, 王娟, 董承光, 李成奇. 利用3VmrMLM方法检测陆地棉株高QTN及QTN-环境互作(QEI)[J]. 作物学报, 2025, 51(10): 2619-2631.
[14] 郑栋, 周仙莉, 滕长才, 侯万伟, 张红岩, 刘玉皎. 基于SSR标记的青海蚕豆品种亲缘关系分析与指纹图谱构建[J]. 作物学报, 2025, 51(1): 79-90.
[15] 匡博文, 韦妳, 刘金典, 陈美燕, 毛兴洁, 段维兴, 杨细平. 基于甘蔗及其近缘属参考基因组开发SSR标记及数据库[J]. 作物学报, 2025, 51(1): 103-116.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2