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作物学报 ›› 2014, Vol. 40 ›› Issue (02): 369-379.doi: 10.3724/SP.J.1006.2014.00369

• 研究简报 • 上一篇    

新疆自育陆地棉品种SSR遗传多样性分析

艾先涛1,2,梁亚军2,沙红2,王俊铎2,郑巨云2,吐尔逊江2,多力坤2,李雪源2,*,华金平3,*   

  1. 1新疆农业大学, 乌鲁木齐 830052；2新疆农业科学院经济作物研究所, 乌鲁木齐 830091；3中国农业大学植物遗传育种系 / 杂种优势研究与利用教育部重点实验室 / 作物遗传改良北京市重点实验室, 北京 100193
  • 收稿日期:2013-01-21 修回日期:2013-08-16 出版日期:2014-02-12 网络出版日期:2013-10-22
  • 通讯作者: 华金平, E-mail: jinping_hua@cau.edu.cn; 李雪源, E-mail: xjmh2338@163.com
  • 基金资助:

    本研究由新疆自然科学基金项目(2012211B47)和国家高技术研究发展计划(863计划)项目(2012AA101108)资助。

Genetic Diversity Analysis on Local Upland Cotton Cultivars in Xinjiang Based on SSR Markers

AI Xian-Tao1, 2,LIANG Ya-Jun2,SHA Hong2,WANG Jun-Duo2,ZHENG Ju-Yun2,Tu-Er-Xun-Jiang2,DUO Li-Kun2,LI Xue-Yuan2, *,HUA Jin-Ping3,*   

  1. 1Xinjiang Agricultural University , Urumqi 830052, China; 2 Economic Crop Research Institute, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China; 3 Department of Plant Genetics and Breeding / Key Laboratory of Crop Heterosis and Utilization of Ministry of Education / Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
  • Received:2013-01-21 Revised:2013-08-16 Published:2014-02-12 Published online:2013-10-22
  • Contact: 华金平, E-mail: jinping_hua@cau.edu.cn; 李雪源, E-mail: xjmh2338@163.com

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摘要：

利用SSR标记对94份新疆自育陆地棉品种的基因组进行分析，研究新疆陆地棉品种的遗传多样性。结果: 从分布于棉花全基因组的206对SSR标记中筛选出54对具有稳定多态性的引物, 共检测出153个多态性位点, 每对引物的等位变异为2~6个，平均为2.93个；基因型多样性(H′)变幅为0.0439–0.7149，平均为0.4491；引物多态信息含量(PIC)为0.0430–0.6640，平均为0.3831。表明SSR标记在品种间可以反映较丰富的遗传多样性信息。94份品种间成对遗传相似系数变幅为0.3846~0.9835, 71.9%的品种相似系数在0.601~0.800内，反映出新疆陆地棉品种间的遗传相似性相对较高。根据UPGMA 聚类分析，在阈值为0.63时，将94份品种划分为2个类群，说明新疆陆地棉品种间遗传关系相对简单，品种的遗传基础相对狭窄，品种遗传组分差异较小，总体上遗传多样性不够丰富；分子聚类结果与品种本身遗传系谱背景和演变趋势吻合度较高，符合品种的真实特性。研究证明，自育品种在分子水平上差异不大，需要努力拓宽品种选育的遗传基础。

关键词: 陆地棉, 遗传背景, SSR, 遗传多样性, 亲缘关系

Abstract:

Xinjiang, the largest prior cotton production region in China, has made remarkable genetic improvement in upland cotton (Gossypium hirsutum L.). There has been a substantial raise in yield potential, cotton-planting area and total production in Xinjiang since 1970s. The new upland cotton cultivars have contributed a lot to yield increase, in which Xinjiang-bred upland cotton varieties play an important role in cotton production in both Northern and Southern Xinjiang. However, seldom research on genetic basis of genetic diversity and pedigree relationship has been evaluated in upland cotton varieties in Xinjiang so far. This study aimed to reveal the genetic diversity of 94 upland cotton varieties in Xinjiang by genome analysis using SSR markers. The results indicated that SSR molecular markers could be employed in revealing the genetic diversity. One hundred and fifty-three polymorphic lociwere detected by amplified with 54 pairs of primers with allelic variation of 2-6 per primer pair and the average of 2.93. The genotypic diversity (H’) spanned from 0.0439 to 0.7149, with the average of 0.4491. The PIC value ranged from 0.0430 to 0.6640, and the average was 0.3831. The coefficients of genetic similarity among 94 tested cultivars varied from 0.3846 to 0.9835. And more than 71.9% of similarity coefficients fell into the interval of 0.601–0.800, indicating that the genetic similarity among the cultivars was high, whereas the genetic diversity might not be abundant. According to UPGMA cluster analysis, 94 tested cultivars were divided into two clusters when the threshold valve was given as 0.63, showing the relatively simple genetic relationship among the upland cotton varieties in Xinjiang, and relatively low genetic diversity and narrow genetic basis. The results of molecular clustering on genetic diversity of upland cotton coincided in large degree with the pedigree relationship. In conclusion, there is no much difference on molecular level among Xinjiang-bred upland cotton varieties. Materials with much wider genetic backgrounds are suggested to be used to diversify the genetic basis of cotton varieties in Xinjiang.

Key words: Upland cotton, Genetic background, SSR, Genetic diversity, Genetic relationship

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