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作物学报 ›› 2014, Vol. 40 ›› Issue (04): 644-649.doi: 10.3724/SP.J.1006.2014.00644
李燕群1,**,高家旭1,**,肖云华1,李秀兰2,蒲翔1,孙昌辉1,王平荣1,邓晓建1,*
LI Yan-Qun1,**,GAO Jia-Xu1,**,XIAO Yun-Hua1,LI Xiu-Lan2,PU Xiang1,SUN Chang-Hui1,WANG Ping-Rong1,DENG Xiao-Jian1,*
摘要：
通过化学诱变获得遗传稳定的水稻黄绿叶突变体ygl80。与野生型亲本10079相比，ygl80突变体在苗期和孕穗期叶片叶绿素分别下降76.64%和54.59%，类胡萝卜素含量分别下降53.85%和41.18%，成熟期株高、每株有效穗数、每穗着粒数、穗长和千粒重分别减少14.8%、16.5%、21.3%、9.1%和7.4%。遗传分析表明，ygl80的突变性状由1对隐性核基因控制。利用(ygl80/浙辐802) F2作为定位群体, 将突变基因定位在第5染色体长臂InDel标记C2和C3之间，遗传距离分别为0.24 cM 和0.39 cM，两标记之间的物理距离约为90 kb，此区间内包含11个预测基因。基因组序列分析发现，ygl80突变体在编码叶绿素合酶的YGL1(LOC_Os05g28200)基因编码区第5027碱基处(位于第14外显子)，碱基C突变为碱基T，使编码蛋白序列第348位的脯氨酸(Pro)突变成亮氨酸(Leu)。该基因是已报道的水稻ygl1黄绿叶突变基因的等位基因。ygl80突变体在整个生育期都表现为黄绿叶，而ygl1突变体在苗期叶片黄化，中期慢慢转绿，后期叶色以及总叶绿素和类胡萝卜素的含量接近野生型，这可能是YGL1基因编码的叶绿素合酶蛋白的氨基酸不同突变位点造成的。
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